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Synaptophysin抗体 [GT2589]

Comparison
Anti-Synaptophysin antibody [GT2589] used in Western Blot (WB). GTX633972
Anti-Synaptophysin antibody [GT2589] used in IHC (Paraffin sections) (IHC-P). GTX633972
Anti-Synaptophysin antibody [GT2589] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX633972
Anti-Synaptophysin antibody [GT2589] used in IHC (Paraffin sections) (IHC-P). GTX633972
Anti-Synaptophysin antibody [GT2589] used in Western Blot (WB). GTX633972
Anti-Synaptophysin antibody [GT2589] used in IHC (Paraffin sections) (IHC-P). GTX633972
Anti-Synaptophysin antibody [GT2589] used in Western Blot (WB). GTX633972
Anti-Synaptophysin antibody [GT2589] used in Western Blot (WB). GTX633972

目录号 GTX633972

1

宿主

 Mouse

克隆

 Monoclonal

克隆编号

 GT2589

同种型

 IgG2b

实验应用

 WB, ICC/IF, IHC-P

种属反应

 Human, Mouse, Rat
产品包装
100 μl (¥4000),
25 μl (¥1700)
查看所有 Synaptophysin 的产品

产品应用

应用说明

*最佳稀释倍数与浓度应由研究人员确认
实验应用 稀释倍数
WB 1:5000-1:20000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
WB: 免疫印迹
ICC/IF: 细胞染色
IHC-P: 石蜡切片
IHC-Fr: 冰冻切片

预期分子量

34 kDa. ( Note )

阳性对照

rat brain

预测种属反应

Bovine, Rhesus Monkey(>80% identity)

产品属性

形式

Liquid

存储溶液

PBS

存放说明

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

浓度

1 mg/ml (Please refer to the vial label for the specific concentration.)

免疫原种属

Human

免疫原

Recombinant protein encompassing a sequence within the C-terminus region of human Synaptophysin. The exact sequence is proprietary.

纯化方式

Affinity purified by Protein A.

偶联

Unconjugated

RRID

AB_2885145

注意事项

仅供实验室使用。不适用于人类或动物的任何临床，治疗或诊断用途。不适合动物或人类食用。

相关讯息

别名

synaptophysin , MRX96 , MRXSYP

细胞定位

Cytoplasmic vesicle , secretory vesicle , synaptic vesicle membrane; Multi-pass membrane protein , Cell junction , synapse , synaptosome

功能与背景

Synaptophysin (p38) is an integral membrane protein of small synaptic vesicles in brain and endocrine cells.[supplied by OMIM]

数据库

研究领域

產品图片

Anti-Synaptophysin antibody [GT2589] used in Western Blot (WB). GTX633972

GTX633972 WB Image

Comparison

Various tissue extracts (50 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with Synaptophysin antibody [GT2589] (GTX633972) diluted at 1:10000. The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody.

Anti-Synaptophysin antibody [GT2589] used in IHC (Paraffin sections) (IHC-P). GTX633972

GTX633972 IHC-P Image

Synaptophysin antibody [GT2589] detects Synaptophysin protein at cytoplasm by immunohistochemical analysis.
Sample: Paraffin-embedded mouse hippocampus.
Synaptophysin stained by Synaptophysin antibody [GT2589] (GTX633972) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-Synaptophysin antibody [GT2589] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX633972

GTX633972 ICC/IF Image

Synaptophysin antibody [GT2589] detects Synaptophysin protein by immunofluorescent analysis.
Sample: DIV9 rat E18 primary hippocampal neuron cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: Synaptophysin stained by Synaptophysin antibody [GT2589] (GTX633972) diluted at 1:500.
Blue: Fluoroshield with DAPI (GTX30920).

Anti-Synaptophysin antibody [GT2589] used in IHC (Paraffin sections) (IHC-P). GTX633972

GTX633972 IHC-P Image

Synaptophysin antibody [GT2589] detects Synaptophysin protein at cell membrane in mouse brain by immunohistochemical analysis.
Sample: Paraffin-embedded mouse brain.
Synaptophysin antibody [GT2589] (GTX633972) diluted at 1:200.

Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-Synaptophysin antibody [GT2589] used in Western Blot (WB). GTX633972

GTX633972 WB Image

Various tissue extracts (50 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with Synaptophysin antibody [GT2589] (GTX633972) diluted at 1:10000. The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody.

Anti-Synaptophysin antibody [GT2589] used in IHC (Paraffin sections) (IHC-P). GTX633972

GTX633972 IHC-P Image

Synaptophysin antibody [GT2589] detects Synaptophysin protein at cell membrane in rat brain by immunohistochemical analysis.
Sample: Paraffin-embedded rat brain.
Synaptophysin antibody [GT2589] (GTX633972) diluted at 1:200.

Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-Synaptophysin antibody [GT2589] used in Western Blot (WB). GTX633972

GTX633972 WB Image

Rat tissue extract (50 μg) was separated by 12% SDS-PAGE, and the membrane was blotted with Synaptophysin antibody [GT2589] (GTX633972) diluted at 1:10000.

Anti-Synaptophysin antibody [GT2589] used in Western Blot (WB). GTX633972

GTX633972 WB Image

Human tissue extract (30 μg) was separated by 12% SDS-PAGE, and the membrane was blotted with Synaptophysin antibody [GT2589] (GTX633972) diluted at 1:1000. The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody.

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产品评论

Synaptophysin antibody [GT2589] 目录号 GTX633972

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实验应用
Western Blot(WB)
( Average 5 based on 1 users reviews)
Anti-Synaptophysin antibody [GT2589] used in Western Blot (WB). GTX633972
Date : Greg Jacobson submitted on 09-Jun-2019
Score :
Application Tested : WB
Sample Species : Ms
Sample : Mouse brain lysate
Amount used : 1 uL in 5mL
Blocking : 5% milk in TBST, 22°C, 1Hr
Primary Antibody : 1:5000, 4°C, 12Hr
条带说明 : Lane 1: 10ug extracted protein mouse brain (0.4%NP-40 lysis) 37oC Control. Lane 2: 10ug extracted protein mouse brain (0.4%NP-40 lysis) 37oC + drug. Lane 3: 10ug extracted protein mouse brain (0.4%NP-40 lysis) 43oC Control. Lane 4: 10ug extracted protein mouse brain (0.4%NP-40 lysis) 43oC + drug.
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SDS
PBS.pdf
产品包装 报价 (¥)
¥ 4000
¥ 1700
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