产品应用
应用说明
*最佳稀释倍数与浓度应由研究人员确认
实验应用 |
稀释倍数 |
1:200-1:1000 |
1:200-1:1000 |
1:200-1:1000 |
1:200-1:400 |
1:10000 |
WB: 免疫印迹
ICC/IF: 细胞染色
IHC-P: 石蜡切片
IHC-Fr: 冰冻切片
预期分子量
产品属性
形式
Liquid
存储溶液
PBS
存放说明
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
浓度
Batch dependent (Please refer to the vial label for the specific concentration.)
免疫原种属
Human
免疫原
A recombinant fragment of human SDC1 (AA: 28-171) expressed in E. coli.
纯化方式
Purified by antigen-affinity chromatography
偶联
Unconjugated
注意事项
仅供实验室使用。不适用于人类或动物的任何临床,治疗或诊断用途。不适合动物或人类食用。
相关讯息
别名
syndecan 1 , CD138 , SDC , SYND1 , syndecan
细胞定位
Membrane,Secreted
功能与背景
The protein encoded by this gene is a transmembrane (type I) heparan sulfate proteoglycan and is a member of the syndecan proteoglycan family. The syndecans mediate cell binding, cell signaling, and cytoskeletal organization and syndecan receptors are required for internalization of the HIV-1 tat protein. The syndecan-1 protein functions as an integral membrane protein and participates in cell proliferation, cell migration and cell-matrix interactions via its receptor for extracellular matrix proteins. Altered syndecan-1 expression has been detected in several different tumor types. While several transcript variants may exist for this gene, the full-length natures of only two have been described to date. These two represent the major variants of this gene and encode the same protein. [provided by RefSeq, Jul 2008]
数据库
研究领域
產品图片
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GTX00451 ELISA Image
ELISA analysis of antigen using GTX00451 Syndecan-1 / CD138 antibody [1A3H4]. Black : Control antigen 100ng Purple : Antigen 10ng Blue : Antigen 50ng Red : Antigen 100ng
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GTX00451 WB Image
WB analysis of various samples using GTX00451 Syndecan-1 / CD138 antibody [1A3H4].
Lane 1 : MCF-7 cell lysate
Lane 2 : HeLa cell lysate
Lane 3 : HepG2 cell lysate
Lane 4 : T47D cell lysate
Lane 5 : SW620 cell lysate
Lane 6 : Jurkat cell lysate
Lane 7 : NIH/3T3 cell lysate
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GTX00451 ICC/IF Image
ICC/IF analysis of HepG2 cells using GTX00451 Syndecan-1 / CD138 antibody [1A3H4]. Green : Primary antibody Blue: DRAQ5 fluorescent DNA dye
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GTX00451 WB Image
WB analysis of various samples using GTX00451 Syndecan-1 / CD138 antibody [1A3H4].
Lane 1 : HEK293 cell lysate
Land 2 : SDC1 (AA: 28-171)-hIgGFc transfected HEK293 cell lysate
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GTX00451 FACS Image
FACS analysis of HepG2 cells using GTX00451 Syndecan-1 / CD138 antibody [1A3H4]. Green : Primary antibody Red : Negative control (without primary antibody)
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GTX00451 IHC-P Image
IHC-P analysis of ovarian cancer tissues using GTX00451 Syndecan-1 / CD138 antibody [1A3H4].
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GTX00451 IHC-P Image
IHC-P analysis of rectum cancer tissues using GTX00451 Syndecan-1 / CD138 antibody [1A3H4].
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文献引用
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产品评论
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