Various whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with TDP43 antibody [GT225] (GTX630196) diluted at 1:1000. The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody.

TARDBP antibody [GT225] validation by siRNA knock-down.
Upperpanel: TARDBP antibody [GT225] GTX630196
Lower panel: GAPDH antibody (GTX100118)
A. 30 μg 293T whole cell lysate/extract
B. 30 μg whole cell lysate/extract of TARDBP shRNA-transfected 293T cells
10 % SDS-PAGE
TARDBP antibody [GT225] (GTX630196) dilution: 1:3000
GAPDH antibody (GTX100118) dilution: 1:10000

TARDBP antibody [GT225] detects TARDBP protein by western blot analysis.
A. 30 μg BCL-1 whole cell lysate/extract
B. 30 μg Raw264.7 whole cell lysate/extract
10 % SDS-PAGE
TARDBP antibody [GT225] (GTX630196) dilution: 1:1000

TDP43 antibody [GT225] detects TDP43 protein at nucleus in mouse brain by immunohistochemical analysis.
Sample: Paraffin-embedded mouse brain.
TDP43 antibody [GT225] (GTX630196) diluted at 1:200.

Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

TARDBP antibody [GT225] detects TARDBP protein by western blot analysis.
A. 30 μg PC-12 whole cell lysate/extract
B. 30 μg Rat2 whole cell lysate/extract
10 % SDS-PAGE
TARDBP antibody [GT225] (GTX630196) dilution: 1:1000

TDP43 antibody [GT225] detects TDP43 protein at nucleus in rat brain by immunohistochemical analysis.
Sample: Paraffin-embedded rat brain.
TDP43 antibody [GT225] (GTX630196) diluted at 1:200.

Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Various whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with TARDBP antibody [GT225] (GTX630196) diluted at 1:500.

Wild-type (WT) and TARDBP knockout (KO) HAP1 cell extracts (50 Âμg) were separated by gradient gel% SDS-PAGE, and the membrane was blotted with TDP43 antibody [GT225] (GTX630196) diluted at 1:500. The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody.
Data provided by YCharOS Inc., an open science company with the mission of characterizing commercially available antibody reagents for all human proteins using knockout technology.