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TDP43抗体 [GT225]

KO/KD
Anti-TDP43 antibody [GT225] used in Western Blot (WB). GTX630196
Anti-TDP43 antibody [GT225] used in Western Blot (WB). GTX630196
Anti-TDP43 antibody [GT225] used in IHC (Paraffin sections) (IHC-P). GTX630196
Anti-TDP43 antibody [GT225] used in Western Blot (WB). GTX630196
Anti-TDP43 antibody [GT225] used in IHC (Paraffin sections) (IHC-P). GTX630196
Anti-TDP43 antibody [GT225] used in Western Blot (WB). GTX630196
Anti-TDP43 antibody [GT225] used in Western Blot (WB). GTX630196
Anti-TDP43 antibody [GT225] used in Western Blot (WB). GTX630196

目录号 GTX630196

文献引用 1
1

宿主

 Mouse

克隆

 Monoclonal

克隆编号

 GT225

同种型

 IgG2b

实验应用

 WB, IHC-P

种属反应

 Human, Mouse, Rat
产品包装
100 μl (¥4000),
25 μl (¥1700)
查看所有 TDP43 的产品

产品应用

应用说明

*最佳稀释倍数与浓度应由研究人员确认
实验应用 稀释倍数
WB 1:500-1:3000
IHC-P 1:100-1:1000
WB: 免疫印迹
ICC/IF: 细胞染色
IHC-P: 石蜡切片
IHC-Fr: 冰冻切片

预期分子量

45 kDa. ( Note )

阳性对照

293T , A431 , HeLa , BCL-1 , Raw264.7 , PC-12 , Rat2 , U87-MG , SK-N-SH , IMR32 , SK-N-AS

预测种属反应

Bovine, Chicken, Xenopus tropicalis(>80% identity)

产品属性

形式

Liquid

存储溶液

PBS, 20% Glycerol

存放说明

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

浓度

1 mg/ml (Please refer to the vial label for the specific concentration.)

免疫原种属

Human

免疫原

Recombinant protein encompassing a sequence within the center region of human TDP43. The exact sequence is proprietary.

纯化方式

Affinity purified by Protein G.

偶联

Unconjugated

RRID

AB_2888198

注意事项

仅供实验室使用。不适用于人类或动物的任何临床，治疗或诊断用途。不适合动物或人类食用。

相关讯息

别名

TAR DNA binding protein , ALS10 , TDP-43

细胞定位

Nucleus

功能与背景

HIV-1, the causative agent of acquired immunodeficiency syndrome (AIDS), contains an RNA genome that produces a chromosomally integrated DNA during the replicative cycle. Activation of HIV-1 gene expression by the transactivator Tat is dependent on an RNA regulatory element (TAR) located downstream of the transcription initiation site. The protein encoded by this gene is a transcriptional repressor that binds to chromosomally integrated TAR DNA and represses HIV-1 transcription. In addition, this protein regulates alternate splicing of the CFTR gene. A similar pseudogene is present on chromosome 20. [provided by RefSeq]

数据库

研究领域

產品图片

Anti-TDP43 antibody [GT225] used in Western Blot (WB). GTX630196

GTX630196 WB Image

KO/KD

Wild-type (WT) and TARDBP knockout (KO) HAP1 cell extracts (50 Âμg) were separated by gradient gel% SDS-PAGE, and the membrane was blotted with TDP43 antibody [GT225] (GTX630196) diluted at 1:500. The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody.
Data provided by YCharOS Inc., an open science company with the mission of characterizing commercially available antibody reagents for all human proteins using knockout technology.

Anti-TDP43 antibody [GT225] used in Western Blot (WB). GTX630196

GTX630196 WB Image

Various whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with TARDBP antibody [GT225] (GTX630196) diluted at 1:500.

Anti-TDP43 antibody [GT225] used in IHC (Paraffin sections) (IHC-P). GTX630196

GTX630196 IHC-P Image

TDP43 antibody [GT225] detects TDP43 protein at nucleus in rat brain by immunohistochemical analysis.
Sample: Paraffin-embedded rat brain.
TDP43 antibody [GT225] (GTX630196) diluted at 1:200.

Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-TDP43 antibody [GT225] used in Western Blot (WB). GTX630196

GTX630196 WB Image

TARDBP antibody [GT225] detects TARDBP protein by western blot analysis.
A. 30 μg PC-12 whole cell lysate/extract
B. 30 μg Rat2 whole cell lysate/extract
10 % SDS-PAGE
TARDBP antibody [GT225] (GTX630196) dilution: 1:1000

Anti-TDP43 antibody [GT225] used in IHC (Paraffin sections) (IHC-P). GTX630196

GTX630196 IHC-P Image

TDP43 antibody [GT225] detects TDP43 protein at nucleus in mouse brain by immunohistochemical analysis.
Sample: Paraffin-embedded mouse brain.
TDP43 antibody [GT225] (GTX630196) diluted at 1:200.

Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-TDP43 antibody [GT225] used in Western Blot (WB). GTX630196

GTX630196 WB Image

TARDBP antibody [GT225] detects TARDBP protein by western blot analysis.
A. 30 μg BCL-1 whole cell lysate/extract
B. 30 μg Raw264.7 whole cell lysate/extract
10 % SDS-PAGE
TARDBP antibody [GT225] (GTX630196) dilution: 1:1000

Anti-TDP43 antibody [GT225] used in Western Blot (WB). GTX630196

GTX630196 WB Image

KO/KD

TARDBP antibody [GT225] validation by siRNA knock-down.
Upperpanel: TARDBP antibody [GT225] GTX630196
Lower panel: GAPDH antibody (GTX100118)
A. 30 μg 293T whole cell lysate/extract
B. 30 μg whole cell lysate/extract of TARDBP shRNA-transfected 293T cells
10 % SDS-PAGE
TARDBP antibody [GT225] (GTX630196) dilution: 1:3000
GAPDH antibody (GTX100118) dilution: 1:10000

Anti-TDP43 antibody [GT225] used in Western Blot (WB). GTX630196

GTX630196 WB Image

Various whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with TDP43 antibody [GT225] (GTX630196) diluted at 1:1000. The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody.

文献引用

Application Reference

Worrall D et al. F1000Res 2023; 12 : 277 The identification of high-performing antibodies for TDP-43 for use in Western Blot, immunoprecipitation and immunofluorescence.
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产品评论

TDP43 antibody [GT225] 目录号 GTX630196

Rating
( Average 5 based on 1 users reviews)
实验应用
Western Blot(WB)
( Average 5 based on 1 users reviews)
Anti-TDP43 antibody [GT225] used in Western Blot (WB). GTX630196
Date : Anonymous submitted on 26-Jul-2022
Score :
Application Tested : WB
Sample Species : Hu
Sample : HAP1 WT and TARDBP KO cells
Amount used : 50μg
Blocking : 5% milk, 1 hr
Primary Antibody : 1:500 in 5% BSA and TBST, 4°C, > 12Hr
Figure Note : Data provided by YCharOS Inc., an open science company with the mission of characterizing commercially available antibody reagents for all human proteins using knockout technology.
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SDS
PBS.pdf
产品包装 报价 (¥)
¥ 4000
¥ 1700
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