产品应用
应用说明
*最佳稀释倍数与浓度应由研究人员确认
| 实验应用 |
稀释倍数 |
| 1:500-1:3000 |
| 1:100-1:1000 |
| 1:100-1:1000 |
WB: 免疫印迹
ICC/IF: 细胞染色
IHC-P: 石蜡切片
IHC-Fr: 冰冻切片
预期分子量
阳性对照
H1299 , HeLa , mouse brain
预测种属反应
Bovine, Chicken(>80% identity)
产品属性
形式
Liquid
存储溶液
PBS, 20% Glycerol
存放说明
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
浓度
1 mg/ml (Please refer to the vial label for the specific concentration.)
免疫原种属
Human
免疫原
Recombinant protein encompassing a sequence within the center region of human TDP43. The exact sequence is proprietary.
纯化方式
Affinity purified by Protein A.
偶联
Unconjugated
RRID
AB_1241379
注意事项
仅供实验室使用。不适用于人类或动物的任何临床，治疗或诊断用途。不适合动物或人类食用。
相关讯息
别名
TAR DNA binding protein , ALS10 , TDP-43
细胞定位
Nucleus
功能与背景
HIV-1, the causative agent of acquired immunodeficiency syndrome (AIDS), contains an RNA genome that produces a chromosomally integrated DNA during the replicative cycle. Activation of HIV-1 gene expression by the transactivator Tat is dependent on an RNA regulatory element (TAR) located downstream of the transcription initiation site. The protein encoded by this gene is a transcriptional repressor that binds to chromosomally integrated TAR DNA and represses HIV-1 transcription. In addition, this protein regulates alternate splicing of the CFTR gene. A similar pseudogene is present on chromosome 20. [provided by RefSeq]
数据库
研究领域
產品图片
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GTX100579 WB Image
Western blot analysis of TDP-43 (GTX100579, upper panel) and beta-actin (GTX109639, lower panel)
Sample (30 ug of whole cell lysate)
A: HeLa mock control
B: HeLa transfected shTDP-43
10% SDS PAGE
GTX100579 diluted at 1:500
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GTX100579 WB Image
Sample (50 ug of whole cell lysate)
A: mouse brain
10% SDS PAGE
GTX100579 diluted at 1:500
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GTX100579 IHC-P Image
Immunohistochemical analysis of paraffin-embedded rat brain, using TARDBP(GTX100579) antibody at 1:500 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min
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GTX100579 WB Image
Sample(30 μg of whole cell lysate)
A:H1299
10% SDS PAGE
GTX100579 diluted at 1:1000
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GTX100579 IHC-P Image
TARDBP antibody detects TARDBP protein at nucleus in mouse brain by immunohistochemical analysis.
Sample: Paraffin-embedded mouse brain.
TARDBP antibody (GTX100579) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
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GTX100579 ICC/IF Image
Confocal immunofluorescence analysis (Olympus FV10i) of paraformaldehyde-fixed HeLa, using TARDBP(GTX100579) antibody (Green) at 1:500 dilution. Alpha-tubulin filaments were labeled with GTX11304 (Red) at 1:2000.
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