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TET1抗体 [N3C1]

Anti-TET1 antibody [N3C1] used in Western Blot (WB). GTX124207
Anti-TET1 antibody [N3C1] used in Western Blot (WB). GTX124207
Anti-TET1 antibody [N3C1] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX124207
Anti-TET1 antibody [N3C1] used in Western Blot (WB). GTX124207
Anti-TET1 antibody [N3C1] used in IHC (Paraffin sections) (IHC-P). GTX124207
Anti-TET1 antibody [N3C1] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX124207
Anti-TET1 antibody [N3C1] used in Western Blot (WB). GTX124207
Anti-TET1 antibody [N3C1] used in IHC (Paraffin sections) (IHC-P). GTX124207
Anti-TET1 antibody [N3C1] used in IHC (Paraffin sections) (IHC-P). GTX124207
Anti-TET1 antibody [N3C1] used in IHC (Paraffin sections) (IHC-P). GTX124207
Anti-TET1 antibody [N3C1] used in IHC (Paraffin sections) (IHC-P). GTX124207
Anti-TET1 antibody [N3C1] used in IHC (Paraffin sections) (IHC-P). GTX124207
Anti-TET1 antibody [N3C1] used in Western Blot (WB). GTX124207
Anti-TET1 antibody [N3C1] used in Western Blot (WB). GTX124207
Anti-TET1 antibody [N3C1] used in Western Blot (WB). GTX124207
Anti-TET1 antibody [N3C1] used in IHC (Paraffin sections) (IHC-P). GTX124207
Anti-TET1 antibody [N3C1] used in Immunohistochemistry (IHC). GTX124207
Anti-TET1 antibody [N3C1] used in ChIP assay (ChIP assay). GTX124207
Anti-TET1 antibody [N3C1] used in Western Blot (WB). GTX124207
Anti-TET1 antibody [N3C1] used in Western Blot (WB). GTX124207

目录号 GTX124207

宿主

Rabbit

克隆

Polyclonal

同种型

IgG

实验应用

WB, ICC/IF, IHC-P, IHC-Wm, IP, ChIP assay, IHC

种属反应

Human, Mouse, Rat, Monkey
产品包装
100 μl (¥4000)
产品说明

摘要

TET1 antibody recognizes TET1 protein, which is a methylcytosine dioxygenase that catalyzes the sequential steps of DNA demethylation. Expression of TET1 protein is spatiotemporally regulated during embryonic development. Abnormal TET1 activation is associated with cancer growth and metastasis.

产品应用

应用说明

*最佳稀释倍数与浓度应由研究人员确认
实验应用 稀释倍数
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
IHC-Wm Assay dependent
IP Assay dependent
ChIP assay Assay dependent
IHC Assay dependent
WB: 免疫印迹
ICC/IF: 细胞染色
IHC-P: 石蜡切片
IHC-Fr: 冰冻切片

预期分子量

235 kDa. ( Note )

阳性对照

DDDDK-human TET1-transfected 293T , 293T , A431 , HeLa , HepG2

产品属性

形式

Liquid

存储溶液

PBS, 20% Glycerol

存放说明

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

浓度

1.05 mg/ml (Please refer to the vial label for the specific concentration.)

免疫原种属

Human

免疫原

Recombinant protein encompassing a sequence within the center region of human TET1. The exact sequence is proprietary.

纯化方式

Purified by antigen-affinity chromatography.

偶联

Unconjugated

RRID

AB_11176491

注意事项

仅供实验室使用。不适用于人类或动物的任何临床,治疗或诊断用途。不适合动物或人类食用。

相关讯息

别名

tet methylcytosine dioxygenase 1 , CXXC6 , LCX , bA119F7.1

细胞定位

Nucleus

功能与背景

Dioxygenase that specifically binds methylcytosine (5mC), a minor base in mammalian DNA found in repetitive DNA elements that is crucial for retrotransposon silencing and mammalian development. Catalyzes the conversion of methylcytosine (5mC) to 5-hydroxymethylcytosine (hmC). The clear function of 5-hydroxymethylcytosine (hmC) is still unclear but it may influence chromatin structure and recruit specific factors or may constitute an intermediate component in cytosine demethylation. 5-hydroxymethylcytosine (hmC) is present in ES cells and is enriched in the brain, especially in Purkinje neurons. May play a role in the fetal development of heart, lung and brain.

数据库

研究领域

產品图片

Anti-TET1 antibody [N3C1] used in Western Blot (WB). GTX124207

GTX124207 WB Image

Non-transfected (–) and transfected (+) 293T whole cell extracts (50 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with TET1 antibody [N3C1] (GTX124207) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-TET1 antibody [N3C1] used in Western Blot (WB). GTX124207

GTX124207 WB Image

Various whole cell extracts (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with TET1 antibody [N3C1] (GTX124207) diluted at 1:2000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-TET1 antibody [N3C1] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX124207

GTX124207 ICC/IF Image

TET1 antibody [N3C1] detects TET1 protein at nucleus by immunofluorescent analysis.
Sample: Mock and transfected 293T cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: TET1 stained by TET1 antibody [N3C1] (GTX124207) diluted at 1:1000.
Blue: Hoechst 33342 staining.

Anti-TET1 antibody [N3C1] used in Western Blot (WB). GTX124207

GTX124207 WB Image

HeLa whole cell and nuclear extracts (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with TET1 antibody [N3C1] (GTX124207) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-TET1 antibody [N3C1] used in IHC (Paraffin sections) (IHC-P). GTX124207

GTX124207 IHC-P Image

Immunohistochemical analysis of paraffin-embedded Hela xenograft, using TET1 (GTX124207) antibody at 1:1000 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-TET1 antibody [N3C1] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX124207

GTX124207 ICC/IF Image

TET1 antibody [N3C1] detects TET1 protein at cytoplasm and nucleus by immunofluorescent analysis.
Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: TET1 stained by TET1 antibody [N3C1] (GTX124207) diluted at 1:500.
Red: alpha Tubulin, a cytoskeleton marker, stained by alpha Tubulin antibody [GT114] (GTX628802) diluted at 1:1000.
Blue: Fluoroshield with DAPI (GTX30920).

Anti-TET1 antibody [N3C1] used in Western Blot (WB). GTX124207

GTX124207 WB Image

TET1 antibody [N3C1] detects TET1 protein by western blot analysis.
A. 30 μg 293T whole cell lysate/extract
B. 30 μg whole cell lysate/extract of DDDDK-human TET1-transfected 293T cells
5% SDS-PAGE
TET1 antibody [N3C1] (GTX124207) dilution: 1:5000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-TET1 antibody [N3C1] used in IHC (Paraffin sections) (IHC-P). GTX124207

GTX124207 IHC-P Image

TET1 antibody [N3C1] detects TET1 protein at nucleus on Human normal prostate tissue by immunohistochemical analysis.
Sample: Paraffin-embedded Human normal prostate tissue.
TET1 antibody [N3C1] (GTX124207) dilution: 1:1000.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-TET1 antibody [N3C1] used in IHC (Paraffin sections) (IHC-P). GTX124207

GTX124207 IHC-P Image

TET1 antibody [N3C1] detects TET1 protein at nucleus in human A549 xenograft by immunohistochemical analysis.
Sample: Paraffin-embedded human A549 xenograft .
TET1 antibody [N3C1] (GTX124207) diluted at 1:250.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-TET1 antibody [N3C1] used in IHC (Paraffin sections) (IHC-P). GTX124207

GTX124207 IHC-P Image

TET1 antibody [N3C1] detects TET1 protein at nucleus by immunohistochemical analysis.
Sample: Paraffin-embedded mouse testis.
TET1 stained by TET1 antibody [N3C1] (GTX124207) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-TET1 antibody [N3C1] used in IHC (Paraffin sections) (IHC-P). GTX124207

GTX124207 IHC-P Image

TET1 antibody [N3C1] detects TET1 protein at nucleus by immunohistochemical analysis.
Sample: Paraffin-embedded rat testis.
TET1 stained by TET1 antibody [N3C1] (GTX124207) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-TET1 antibody [N3C1] used in IHC (Paraffin sections) (IHC-P). GTX124207

GTX124207 IHC-P Image

TET1 antibody [N3C1] detects TET1 protein at nucleus by immunohistochemical analysis.
Sample: Paraffin-embedded mouse testis.
TET1 stained by TET1 antibody [N3C1] (GTX124207) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-TET1 antibody [N3C1] used in Western Blot (WB). GTX124207

GTX124207 WB Image

The data was published in the journal Int J Endocrinol in 2020.PMID: 32089682

Anti-TET1 antibody [N3C1] used in Western Blot (WB). GTX124207

GTX124207 WB Image

The data was published in the journal Clin Epigenetics in 2016.PMID: 26973719

Anti-TET1 antibody [N3C1] used in Western Blot (WB). GTX124207

GTX124207 WB Image

The data was published in the journal BMC Cancer in 2018.PMID: 30045709

Anti-TET1 antibody [N3C1] used in IHC (Paraffin sections) (IHC-P). GTX124207

GTX124207 IHC-P Image

The data was published in the journal Clin Epigenetics in 2018. PMID: 30075814

Anti-TET1 antibody [N3C1] used in Immunohistochemistry (IHC). GTX124207

GTX124207 IHC Image

The data was published in the journal Clin Epigenetics in 2018. PMID: 30075814

Anti-TET1 antibody [N3C1] used in ChIP assay (ChIP assay). GTX124207

GTX124207 ChIP assay Image

The data was published in the journal Sci Rep in 2016. PMID: 27708396

Anti-TET1 antibody [N3C1] used in Western Blot (WB). GTX124207

GTX124207 WB Image

The data was published in the journal Int J Endocrinol in 2020.PMID: 32089682

Anti-TET1 antibody [N3C1] used in Western Blot (WB). GTX124207

GTX124207 WB Image

The data was published in the journal Clin Epigenetics in 2016.PMID: 26973719

文献引用

产品评论

TET1 antibody [N3C1] 目录号 GTX124207

Rating ( Average 5 based on 2 users reviews)
实验应用
IHC (Paraffin sections)(IHC-P) ( Average 5 based on 2 users reviews)
Date : Anonymous submitted on 23-Dec-2020
Score :
Application Tested : IHC-P
Sample Species : Hu
Figure Note : The data was peer reviewed and published in the journal Clin Epigenetics in 2018. PMID: 30075814
Date : Anonymous submitted on 14-Oct-2016
Score :
Application Tested : IHC-P
Sample Species : Hu
Sample : human pancreatic ductal adenocarcinoma
Fixation : 10% paraformaldehyde
Primary Antibody : 1:200, 25°C, 2Hr
Application Reference
SDS
PBS.pdf
Glycerol.pdf
Proclin.pdf
产品包装 报价 (¥)
¥ 4000
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