GeneTex中国官方网站
  • China

TET2抗体 [N2-2], N-term

KO/KD Overexpression
Anti-TET2 antibody [N2-2], N-term used in Western Blot (WB). GTX124205
Anti-TET2 antibody [N2-2], N-term used in ChIP assay (ChIP assay). GTX124205
Anti-TET2 antibody [N2-2], N-term used in IHC (Paraffin sections) (IHC-P). GTX124205
Anti-TET2 antibody [N2-2], N-term used in Immunoprecipitation (IP). GTX124205
Anti-TET2 antibody [N2-2], N-term used in IHC (Paraffin sections) (IHC-P). GTX124205
Anti-TET2 antibody [N2-2], N-term used in Western Blot (WB). GTX124205
Anti-TET2 antibody [N2-2], N-term used in IHC (Paraffin sections) (IHC-P). GTX124205
Anti-TET2 antibody [N2-2], N-term used in IHC (Paraffin sections) (IHC-P). GTX124205
Anti-TET2 antibody [N2-2], N-term used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX124205
Anti-TET2 antibody [N2-2], N-term used in Western Blot (WB). GTX124205

目录号 GTX124205

文献引用 17

宿主

 Rabbit

克隆

 Polyclonal

同种型

 IgG

实验应用

 WB, ICC/IF, IHC-P, IP, ChIP assay

种属反应

 Human, Mouse
产品包装
100 μl (¥4000),
25 μl (¥1700)
查看所有 TET2 的产品

产品应用

应用说明

*最佳稀释倍数与浓度应由研究人员确认
实验应用 稀释倍数
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
IP 1:100-1:500
ChIP assay Assay dependent
WB: 免疫印迹
ICC/IF: 细胞染色
IHC-P: 石蜡切片
IHC-Fr: 冰冻切片

预期分子量

224 kDa. ( Note )

阳性对照

human TET2-transfected 293T cells

产品属性

形式

Liquid

存储溶液

PBS, 20% Glycerol

存放说明

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

浓度

1.55 mg/ml (Please refer to the vial label for the specific concentration.)

免疫原种属

Human

免疫原

Recombinant protein encompassing a sequence within the N-terminus region of human TET2. The exact sequence is proprietary.

纯化方式

Purified by antigen-affinity chromatography.

偶联

Unconjugated

RRID

AB_11166461

注意事项

仅供实验室使用。不适用于人类或动物的任何临床，治疗或诊断用途。不适合动物或人类食用。

相关讯息

别名

tet methylcytosine dioxygenase 2 , KIAA1546 , MDS

功能与背景

The protein encoded by this gene is a methylcytosine dioxygenase that catalyzes the conversion of methylcytosine to 5-hydroxymethylcytosine. The encoded protein is involved in myelopoiesis, and defects in this gene have been associated with several myeloproliferative disorders. Two variants encoding different isoforms have been found for this gene. [provided by RefSeq, Mar 2011]

数据库

研究领域

產品图片

Anti-TET2 antibody [N2-2], N-term used in Western Blot (WB). GTX124205

GTX124205 WB Image

KO/KD

Wild-type (WT) and TET2 knockout (KO) HeLa cell extracts (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with TET2 antibody [N2-2], N-term (GTX124205) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-TET2 antibody [N2-2], N-term used in ChIP assay (ChIP assay). GTX124205

GTX124205 ChIP assay Image

Cross-linked ChIP was performed with U2OS chromatin extract and 5 μg of either control rabbit IgG or anti-TET2 antibody. The precipitated DNA was detected by PCR with primer set targeting to CCND2.
ChIP experiment and primer designs are based on Nat Commun. 2013;4:2166.

Anti-TET2 antibody [N2-2], N-term used in IHC (Paraffin sections) (IHC-P). GTX124205

GTX124205 IHC-P Image

TET2 antibody [N2-2], N-term detects TET2 protein at nucleus by immunohistochemical analysis.
Sample: Paraffin-embedded human breast carcinoma.
TET2 stained by TET2 antibody [N2-2], N-term (GTX124205) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-TET2 antibody [N2-2], N-term used in Immunoprecipitation (IP). GTX124205

GTX124205 IP Image

Immunoprecipitation of TET2 protein from 293T whole cell extracts using 5 μg of TET2 antibody [N2-2], N-term (GTX124205).
Western blot analysis was performed using TET2 antibody [N2-2], N-term (GTX124205) diluted at 1:500.
EasyBlot HRP-conjugated anti rabbit IgG antibody (GTX221666-01) was used to detect the primary antibody.

Anti-TET2 antibody [N2-2], N-term used in IHC (Paraffin sections) (IHC-P). GTX124205

GTX124205 IHC-P Image

TET2 antibody [N2-2], N-term detects TET2 protein on human cervical carcinoma by immunohistochemical analysis.
Sample: Paraffin-embedded cervical carcinoma.
TET2 antibody [N2-2], N-term (GTX124205) dilution: 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-TET2 antibody [N2-2], N-term used in Western Blot (WB). GTX124205

GTX124205 WB Image

Overexpression

TET2 antibody detects TET2 protein by western blot analysis.
A. 30 μg 293T whole cell lysate/extract
B. 30 μg whole cell lysate/extract of DDDDK-human TET2-transfected 293T cells
5% SDS-PAGE
TET2 antibody (GTX124205) dilution: 1:5000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-TET2 antibody [N2-2], N-term used in IHC (Paraffin sections) (IHC-P). GTX124205

GTX124205 IHC-P Image

TET2 antibody [N2-2], N-term detects TET2 protein at nucleus by immunohistochemical analysis.
Sample: Paraffin-embedded mouse liver.
TET2 stained by TET2 antibody [N2-2], N-term (GTX124205) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-TET2 antibody [N2-2], N-term used in IHC (Paraffin sections) (IHC-P). GTX124205

GTX124205 IHC-P Image

TET2 antibody [N2-2], N-term detects TET2 protein at nucleus by immunohistochemical analysis.
Sample: Paraffin-embedded human breast carcinoma.
TET2 stained by TET2 antibody [N2-2], N-term (GTX124205) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-TET2 antibody [N2-2], N-term used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX124205

GTX124205 ICC/IF Image

TET2 antibody [N2-2], N-term detects TET2 protein at nucleus by immunofluorescent analysis.
Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: TET2 stained by TET2 antibody [N2-2], N-term (GTX124205) diluted at 1:500.
Red: alpha Tubulin, a cytoskeleton marker, stained by alpha Tubulin antibody [GT114] (GTX628802) diluted at 1:1000.

Anti-TET2 antibody [N2-2], N-term used in Western Blot (WB). GTX124205

GTX124205 WB Image

Various whole cell extracts (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with TET2 antibody [N2-2], N-term (GTX124205) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

文献引用

Application Reference

Lv H et al. Nat Commun 2024; 15 (1) : 6 TET2-mediated tumor cGAS triggers endothelial STING activation to regulate vasculature remodeling and anti-tumor immunity in liver cancer
实验应用 : WB
种属反应 : Mouse
Tung Chao et al. Autophagy 2021; 17 (11) : 3444-3460 Autophagy restricts mitochondrial DNA damage-induced release of ENDOG (endonuclease G) to regulate genome stability.
实验应用 : WB
种属反应 : Human
Shirui Huang et al. Cancer Cell Int 2021; 21 (1) : 241 miR-190 promotes malignant transformation and?progression of human urothelial cells through CDKN1B/p27 inhibition.
实验应用 : WB
种属反应 : Human
Yamashita H et al. Virchows Arch 2021; (Epub) Epigenetic upregulation of TET2 is an independent poor prognostic factor for intrahepatic cholangiocarcinoma.
实验应用 : IHC-P
种属反应 : Human
Chen W et al. Cell Rep 2021; 37 (5) : 109912 Fetal growth restriction impairs hippocampal neurogenesis and cognition via Tet1 in offspring.
实验应用 : WB
种属反应 : Mouse
显示更多 +
我要回报文献

产品评论

目前没有使用 TET2 antibody [N2-2], N-term (GTX124205) 的产品评论被收录。快发表第一篇产品评论吧！
我要评论产品
Application Reference
Lv H et al. Nat Commun 2024; 15 (1):6 TET2-mediated tumor cGAS triggers endothelial STING activation to regulate vasculature remodeling and anti-tumor immunity in liver cancer
实验应用 : WB
种属反应 : Mouse
Tung Chao et al. Autophagy 2021; 17 (11):3444-3460 Autophagy restricts mitochondrial DNA damage-induced release of ENDOG (endonuclease G) to regulate genome stability.
实验应用 : WB
种属反应 : Human
Shirui Huang et al. Cancer Cell Int 2021; 21 (1):241 miR-190 promotes malignant transformation and?progression of human urothelial cells through CDKN1B/p27 inhibition.
实验应用 : WB
种属反应 : Human
Yamashita H et al. Virchows Arch 2021; (Epub) Epigenetic upregulation of TET2 is an independent poor prognostic factor for intrahepatic cholangiocarcinoma.
实验应用 : IHC-P
种属反应 : Human
Chen W et al. Cell Rep 2021; 37 (5):109912 Fetal growth restriction impairs hippocampal neurogenesis and cognition via Tet1 in offspring.
实验应用 : WB
种属反应 : Mouse
Williamson SM et al. J Insect Physiol 2021; 134:104298 Epigenetic underpinnings of freeze avoidance in the goldenrod gall moth, Epiblema scudderiana.
实验应用 : WB
Wu SL et al. Genomics Proteomics Bioinformatics 2021; Genome-wide 5-hydroxymethylcytosine Profiling Analysis Identifies MAP7D1 as A Novel Regulator of Lymph Node Metastasis in Breast Cancer.
实验应用 : IHC-P
种属反应 : Human
Huang F et al. Aging (Albany NY) 2020; 12 (17):16759-16774 HDAC4 inhibition disrupts TET2 function in high-risk MDS and AML.
实验应用 : WB
种属反应 : Mouse
Gu Y et al. FASEB J 2020; (Epub) Hydrogen sulfide attenuates renal fibrosis by inducing TET-dependent DNA demethylation on Klotho promoter.
实验应用 : IHC-P
种属反应 : Human
Xu Y et al. Front Endocrinol (Lausanne) 2020; 11:361 Changes in DNA 5-Hydroxymethylcytosine Levels and the Underlying Mechanism in Non-functioning Pituitary Adenomas.
实验应用 : IHC-P
种属反应 : Human
Pei YF et al. Am J Pathol 2019; (Epub) Methyl-CpG Binding Domain Protein 2 Inhibits the Malignant Characteristic of Lung Adenocarcinoma through the Epigenetic Modulation of 10 to 11 Translocation 1 and miR-200s.
实验应用 : WB
种属反应 : Human
Chatterjee B et al. Biochim Biophys Acta Gene Regul Mech 2018; 1861 (11):1048-1061 DNA Demethylation by DNMT3A and DNMT3B in vitro and of Methylated Episomal DNA in Transiently Transfected Cells.
实验应用 : WB
种属反应 : Mouse
Sun J et al. Cell Stem Cell 2018; 23 (3):355-369.e9 SIRT1 Activation Disrupts Maintenance of Myelodysplastic Syndrome Stem and Progenitor Cells by Restoring TET2 Function.
实验应用 : WB
Zhou Y et al. Cell Physiol Biochem 2018; 45 (6):2548-2559 Ginsenoside 20(S)-Rg3 Inhibits the Warburg Effect Via Modulating DNMT3A/ MiR-532-3p/HK2 Pathway in Ovarian Cancer Cells.
实验应用 : WB
种属反应 : Human
Ancey P et al. Stem Cell Reporters 2017; 9:264-278 TET-Catalyzed 5-Hydroxymethylation Precedes HNF4A Promoter Choice during Differentiation of Bipotent Liver Progenitors
实验应用 : ChIP assay
种属反应 : Human
Ni K et al. Hum Reprod 2016; (Epub) TET enzymes are successively expressed during human spermatogenesis and their expression level is pivotal for male fertility.
实验应用 : WB, IHC-P
种属反应 : Human
Mahaira LG et al. Stem Cells Dev 2014; (Epub) IGF2BP1 expression in human mesenchymal stem cells significantly affects their proliferation and is under the epigenetic control of TET1/2 demethylases.
实验应用 : ICC/IF
种属反应 : Human
SDS
PBS.pdf
Glycerol.pdf
Proclin.pdf
产品包装 报价 (¥)
¥ 4000
¥ 1700
联系经销商
欣博盛生物科技有限公司
欣博盛生物科技有限公司
(全国热线) 4006-800-892 / (深圳) 0755-26755892 / (北京) 010-88594029 / (上海) 021-34613729 / (广州) 18024516375 / (香港) +852-69410778
http://www.neobioscience.com
genetex@neobioscience.com