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TET3抗体 [C3], C-term

Anti-TET3 antibody [C3], C-term used in Immunoprecipitation (IP). GTX121453
Anti-TET3 antibody [C3], C-term used in Western Blot (WB). GTX121453
Anti-TET3 antibody [C3], C-term used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX121453
Anti-TET3 antibody [C3], C-term used in IHC (Paraffin sections) (IHC-P). GTX121453
Anti-TET3 antibody [C3], C-term used in Western Blot (WB). GTX121453
Anti-TET3 antibody [C3], C-term used in Western Blot (WB). GTX121453
Anti-TET3 antibody [C3], C-term used in Western Blot (WB). GTX121453

目录号 GTX121453

文献引用 32

宿主

 Rabbit

克隆

 Polyclonal

同种型

 IgG

实验应用

 WB, ICC/IF, IHC-P, IHC-Wm, IP, ChIP assay

种属反应

 Human, Mouse, Bovine, Xenopus laevis
产品包装
100 μl (¥4000),
25 μl (¥1700)
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产品说明

摘要

TET3 antibody recognizes TET3 protein (predicted molecular weight of 194 kDa), a dioxygenase involved in active DNA demethylation that converts 5-methylcytosine (5mC) to 5-hydroxymethylcytosine (5hmC) and subsequently to 5-formylcytosine (5fC) and 5-carboxylcytosine (5caC). Like TET1 and TET2, TET3 appears to impact epigenetic regulation of gene expression. It is also involved in early embryogenesis, neuronal function, and other processes.

产品应用

应用说明

*最佳稀释倍数与浓度应由研究人员确认
实验应用 稀释倍数
WB 1:1000-1:10000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
IHC-Wm Assay dependent
IP 1:500-1:1000
ChIP assay Assay dependent
WB: 免疫印迹
ICC/IF: 细胞染色
IHC-P: 石蜡切片
IHC-Fr: 冰冻切片

预期分子量

179 kDa. ( Note )

预测种属反应

Xenopus tropicalis(>80% identity)

产品属性

形式

Liquid

存储溶液

PBS, 1% BSA, 20% Glycerol

存放说明

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

浓度

0.14 mg/ml (Please refer to the vial label for the specific concentration.)

免疫原种属

Human

免疫原

Recombinant protein encompassing a sequence within the C-terminus region of human TET3. The exact sequence is proprietary.

纯化方式

Purified by antigen-affinity chromatography.

偶联

Unconjugated

RRID

AB_10723106

注意事项

仅供实验室使用。不适用于人类或动物的任何临床，治疗或诊断用途。不适合动物或人类食用。

相关讯息

别名

tet methylcytosine dioxygenase 3 , hCG_40738

细胞定位

Nucleus,Cytoplasm

功能与背景

Members of the ten-eleven translocation (TET) gene family, including TET3, play a role in the DNA methylation process (Langemeijer et al., 2009 [PubMed 19923888]).[supplied by OMIM, Nov 2010]

数据库

研究领域

產品图片

Anti-TET3 antibody [C3], C-term used in Immunoprecipitation (IP). GTX121453

GTX121453 IP Image

TET3 antibody immunoprecipitates TET3 protein in IP experiments. IP Sample: cell lysate/extract of TET3 gene transfected 293T cells A. Cell lysate/extract of transfected 293T cell B. Control with 2 μg of preimmune rabbit IgG C. Immunoprecipitation of TET3 by 2 μg of TET3 antibody (GTX121453) 5% SDS-PAGE The immunoprecipitated TET3 protein was detected by TET3 antibody (GTX121453) diluted at 1:1000. EasyBlot anti-rabbit IgG (GTX221666-01) was used as a secondary reagent.

Anti-TET3 antibody [C3], C-term used in Western Blot (WB). GTX121453

GTX121453 WB Image

Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with TET3 antibody [C3], C-term (GTX121453) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-TET3 antibody [C3], C-term used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX121453

GTX121453 ICC/IF Image

TET3 antibody [C3], C-term detects TET3 protein at cytoplasm and nucleus by immunofluorescent analysis.
Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: TET3 protein stained by TET3 antibody [C3], C-term (GTX121453) diluted at 1:400.
Red: phalloidin, a cytoskeleton marker, diluted at 1:50.

Anti-TET3 antibody [C3], C-term used in IHC (Paraffin sections) (IHC-P). GTX121453

GTX121453 IHC-P Image

TET3 antibody [C3], C-term detects TET3 protein at nucleus by immunohistochemical analysis.
Sample: Paraffin-embedded mouse colon.
TET3 stained by TET3 antibody [C3], C-term (GTX121453) diluted at 1:500.
Antigen Retrieval: Trilogy™M (EDTA based) buffer, 15min

Anti-TET3 antibody [C3], C-term used in Western Blot (WB). GTX121453

GTX121453 WB Image

Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with TET3 antibody [C3], C-term (GTX121453) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.

Anti-TET3 antibody [C3], C-term used in Western Blot (WB). GTX121453

GTX121453 WB Image

The data was published in the journal Oncogene in 2019. PMID: 31089260

Anti-TET3 antibody [C3], C-term used in Western Blot (WB). GTX121453

GTX121453 WB Image

The data was published in the journal Int J Endocrinol in 2020.PMID: 32089682

文献引用

Application Reference

Zhang J et al. Signal Transduct Target Ther 2024; 9 (1) : 65 Tet methylcytosine dioxygenase 2 (TET2) deficiency elicits EGFR-TKI (tyrosine kinase inhibitors) resistance in non-small cell lung cancer.
B Liu et al. Diabetologia 2024; Skeletal muscle TET3 promotes insulin resistance through destabilisation of PGC-1α
实验应用 : WB
种属反应 : Human
Song J et al. Mol Metab 2023; 78 : 101828 Let-7 suppresses liver fibrosis by inhibiting hepatocyte apoptosis and TGF-β production.
Ye B et al. Cell Mol Immunol 2022; 19 (5) : 619-633 Induction of functional neutrophils from mouse fibroblasts by thymidine through enhancement of Tet3 activity.
Cheng S et al. Cell 2022; 185 (17) : 3169-3185.e20 The intrinsic and extrinsic effects of TET proteins during gastrulation.
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Application Reference
Zhang J et al. Signal Transduct Target Ther 2024; 9 (1):65 Tet methylcytosine dioxygenase 2 (TET2) deficiency elicits EGFR-TKI (tyrosine kinase inhibitors) resistance in non-small cell lung cancer.
B Liu et al. Diabetologia 2024; Skeletal muscle TET3 promotes insulin resistance through destabilisation of PGC-1α
实验应用 : WB
种属反应 : Human
Song J et al. Mol Metab 2023; 78:101828 Let-7 suppresses liver fibrosis by inhibiting hepatocyte apoptosis and TGF-β production.
Ye B et al. Cell Mol Immunol 2022; 19 (5):619-633 Induction of functional neutrophils from mouse fibroblasts by thymidine through enhancement of Tet3 activity.
Xie D et al. J Clin Invest 2022; 132 (19) TET3 epigenetically controls feeding and stress response behaviors via AGRP neurons.
Cheng S et al. Cell 2022; 185 (17):3169-3185.e20 The intrinsic and extrinsic effects of TET proteins during gastrulation.
Ciesielski P et al. Int J Mol Sci 2021; 22 (24) TET3- and OGT-Dependent Expression of Genes Involved in Epithelial-Mesenchymal Transition in Endometrial Cancer.
Chen W et al. Cell Rep 2021; 37 (5):109912 Fetal growth restriction impairs hippocampal neurogenesis and cognition via Tet1 in offspring.
实验应用 : WB
种属反应 : Mouse
Aggarwal RK et al. Proc Natl Acad Sci U S A 2021; 118 (39) Functional succinate dehydrogenase deficiency is a common adverse feature of clear cell renal cancer.
实验应用 : WB
种属反应 : Human
Nishikawa K et al. EMBO Rep 2021;:e53035 Osteoclasts adapt to physioxia perturbation through DNA demethylation.
实验应用 : WB
种属反应 : Mouse
Kawakubo-Yasukochi T et al. Mol Metab 2021; 54:101360 Hepatic glycogenolysis is determined by maternal high-calorie diet via methylation of Pygl and it is modified by oteocalcin administration in mice.
实验应用 : ChIP assay
种属反应 : Mouse
Williamson SM et al. J Insect Physiol 2021; 134:104298 Epigenetic underpinnings of freeze avoidance in the goldenrod gall moth, Epiblema scudderiana.
实验应用 : WB
Gu Y et al. FASEB J 2020; (Epub) Hydrogen sulfide attenuates renal fibrosis by inducing TET-dependent DNA demethylation on Klotho promoter.
实验应用 : IHC-P
种属反应 : Human
Yu S et al. Int J Endocrinol 2020; 2020:3909610 TET1 is a Tumor Suppressor That Inhibits Papillary Thyroid Carcinoma Cell Migration and Invasion.
实验应用 : WB
种属反应 : Human
Xu Y et al. Cell Rep 2020; 30 (5):1310-1318.e5 A Positive Feedback Loop of TET3 and TGF-β1 Promotes Liver Fibrosis.
实验应用 : WB
种属反应 : Mouse
Li Q et al. Mol Reprod Dev 2019; (Epub) DNA methylation regulated by ascorbic acids in yak preimplantation embryo helps to improve blastocyst quality.
实验应用 : IHC-Wm
Cao T et al. Oncogene 2019; (Epub) H19 lncRNA identified as a master regulator of genes that drive uterine leiomyomas.
实验应用 : WB
种属反应 : Human
Pei YF et al. Am J Pathol 2019; (Epub) Methyl-CpG Binding Domain Protein 2 Inhibits the Malignant Characteristic of Lung Adenocarcinoma through the Epigenetic Modulation of 10 to 11 Translocation 1 and miR-200s.
实验应用 : WB
种属反应 : Human
Selimoglu-Buet D et al. Nat Commun 2018; 9 (1):5455 A miR-150/TET3 pathway regulates the generation of mouse and human non-classical monocyte subset.
实验应用 : WB
种属反应 : Human
Liu C et al. Int J Neuropsychopharmacol 2017; (Epub) Retrieval-induced Upregulation of Tet3 in Pyramidal Neurons of the Dorsal Hippocampus Mediates Cocaine-Associated Memory Reconsolidation.
实验应用 : WB
Lu F et al. J Virol 2017; (Epub) Coordinate Regulation of TET2 and EBNA2 Control DNA Methylation State of Latent Epstein-Barr Virus.
实验应用 : WB
种属反应 : Human
Ni K et al. Hum Reprod 2016; (Epub) TET enzymes are successively expressed during human spermatogenesis and their expression level is pivotal for male fertility.
实验应用 : WB, IHC-P
种属反应 : Human
Tampe B et al. EBioMedicine 2015; 2 (1):19-36 Induction of Tet3-dependent Epigenetic Remodeling by Low-dose * Attenuates Progression of Chronic Kidney Disease.
实验应用 : WB
种属反应 : Mouse
Xu X et al. Cardiovasc Res 2015; 105 (3):279-91 Epigenetic balance of aberrant Rasal1 promoter methylation and hydroxymethylation regulates cardiac fibrosis.
实验应用 : ChIP assay
种属反应 : Human
Ichimura N et al. Cancer Prev Res (Phila) 2015; 8 (8):702-11 Aberrant TET1 Methylation Closely Associated with CpG Island Methylator Phenotype in Colorectal Cancer.
实验应用 : WB
种属反应 : Human
Kang J et al. Proc Natl Acad Sci U S A 2015; 112 (31):E4236-45 Simultaneous deletion of the methylcytosine oxidases Tet1 and Tet3 increases transcriptome variability in early embryogenesis.
Zhang Q et al. Nature 2015; (Epub) Tet2 is required to resolve inflammation by recruiting Hdac2 to specifically repress IL-6.
实验应用 : WB
种属反应 : Mouse
Loriot A et al. Epigenetics 2014; 9 (8):1163-71 A novel cancer-germline transcript carrying pro-metastatic miR-105 and TET-targeting miR-767 induced by DNA hypomethylation in tumors.
实验应用 : WB
种属反应 : Human
Rampal R et al. Cell Rep 2014; DNA Hydroxymethylation Profiling Reveals that WT1 Mutations Result in Loss of TET2 Function in Acute Myeloid Leukemia.
实验应用 : WB
种属反应 : Human
Page-Lariviere F et al. Cell Reprogram 2014; 16 (1):40-53 Spatiotemporal expression of DNA demethylation enzymes and histone demethylases in bovine embryos.
实验应用 : WB, IHC-Wm
种属反应 : Bovine
Cheng J et al. Cell Rep 2013; 5 (2):471-81 An extensive network of TET2-targeting MicroRNAs regulates malignant hematopoiesis.
实验应用 : WB
种属反应 : Human
Xu Y et al. Cell 2012; 151 (6):1200-13 Tet3 CXXC domain and dioxygenase activity cooperatively regulate key genes for Xenopus eye and neural development.
实验应用 : WB
种属反应 : Xenopus laevis
SDS
PBS.pdf
Glycerol.pdf
Proclin.pdf
产品包装 报价 (¥)
¥ 4000
¥ 1700
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