产品说明
摘要
The GTX14696 is supplied as a ready‐to‐use solution. Peroxidase reacts with the substrate solution to produce a soluble blue reaction product that can be read at 370 nm or 620‐650 nm for ELISA applications. Stop solutions can be added to prevent further color development and reading the absorbance values at the desired wavelengths.
A soluble yellow product develops after stopping and stabilizing with a 450 nm stop solution.
The developing soluble blue product can be stopped and stabilized by using a 650 nm stop solution.
产品应用
应用说明
Allow the substrate to equilibrate at room temperature (~25ºC) prior to use.
End-Point Assays:
(1) Add 100 μL of equilibrated substrate solution to each microwell.
(2) Allow the substrate reaction to develop a blue soluble reaction by incubating for the optimal time determined for the assay.
(3) The reaction should be stopped by using a 450 nm or 650 nm stop solution.
(4) Measure the absorbance at 450 nm or 650 nm of each microwell within 1 hour.
Kinetic Assays:
(1) Add 100 μL of equilibrated substrate solution to each microtiter plate well.
(2) Measure the absorbance at 650 nm at multiple time points.
(3) Calculate the rate of absorbance change for each microwell.
产品属性
形式
Liquid
存放说明
Stable for 2.5 years at 4ºC. Protect from light.
注意事项
仅供实验室使用。不适用于人类或动物的任何临床，治疗或诊断用途。不适合动物或人类食用。
相关讯息
功能与背景
GeneTex TMB substrate is supplied as a one-component- ready-to-use solution. It is colorless or very light yellow in appearance. When TMB reacts with peroxidase, a blue liquid is yielded (oxidized product). The reaction can be terminated using 0.6N HCl. GeneTex TMB substrate is not recommended for membrane or immunohistochemistry applications where a colored precipitate is desired.
文献引用
目前没有使用 TMB Substrate (GTX14696) 的文献被收录。快来回报我们第一篇参考文献吧！
产品评论
目前没有使用 TMB Substrate (GTX14696) 的产品评论被收录。快发表第一篇产品评论吧！
