产品应用
应用说明
*最佳稀释倍数与浓度应由研究人员确认
实验应用 |
稀释倍数 |
1:5000-1:50000 |
Assay dependent |
WB: 免疫印迹
ICC/IF: 细胞染色
IHC-P: 石蜡切片
IHC-Fr: 冰冻切片
预期分子量
产品说明
This antibody was raised against mouse TMEM119 Intracellular domain.
产品属性
形式
Liquid
存储溶液
PBS
存放说明
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
浓度
1 mg/ml (Please refer to the vial label for the specific concentration.)
免疫原种属
Mouse
免疫原
Recombinant protein encompassing a sequence within the Intracellular domain of mouse TMEM119. The exact sequence is proprietary.
纯化方式
Affinity purified by Protein A.
偶联
Unconjugated
注意事项
仅供实验室使用。不适用于人类或动物的任何临床,治疗或诊断用途。不适合动物或人类食用。
相关讯息
别名
transmembrane protein 119 , AW208946 , BC025600 , obif
细胞定位
Cell membrane,Cytoplasm,Endoplasmic reticulum membrane
功能与背景
Involved in several processes, including endochondral ossification; negative regulation of bone resorption; and positive regulation of macromolecule metabolic process. Acts upstream of or within osteoblast differentiation. Located in endoplasmic reticulum and plasma membrane. Is expressed in several structures, including alimentary system; brain; genitourinary system; limb; and skeleton. Orthologous to human TMEM119 (transmembrane protein 119). [provided by Alliance of Genome Resources, Apr 2022]
数据库
研究领域
產品图片
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GTX639083 WB Image
Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with TMEM119 antibody [HL2627] (GTX639083) diluted at 1:50000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
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GTX639083 WB Image
Mouse tissue extract (50 μg) was separated by 12% SDS-PAGE, and the membrane was blotted with TMEM119 antibody [HL2627] (GTX639083) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
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GTX639083 IHC-P Image
TMEM119 antibody [HL2627] detects TMEM119 protein at cell membrane by immunohistochemical analysis.Sample: Paraffin-embedded mouse brain.TMEM119 stained by TMEM119 antibody [HL2627] (GTX639083) diluted at 1:100.Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
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