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TMS1抗体 [N1C3]

Overexpression
Anti-TMS1 antibody [N1C3] used in Western Blot (WB). GTX102474
Anti-TMS1 antibody [N1C3] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX102474
Anti-TMS1 antibody [N1C3] used in Western Blot (WB). GTX102474

目录号 GTX102474

文献引用 2

宿主

 Rabbit

克隆

 Polyclonal

同种型

 IgG

实验应用

 WB, ICC/IF, IHC-P, IHC-Fr

种属反应

 Human
产品包装
100 μl (¥4000),
25 μl (¥1700)
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产品应用

应用说明

*最佳稀释倍数与浓度应由研究人员确认
实验应用 稀释倍数
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P Assay dependent
IHC-Fr Assay dependent
WB: 免疫印迹
ICC/IF: 细胞染色
IHC-P: 石蜡切片
IHC-Fr: 冰冻切片

预期分子量

22 kDa. ( Note )

阳性对照

THP-1 , DDDDK-tagged PYCARD-transfected 293T

预测种属反应

Rhesus Monkey(>80% identity)

产品属性

形式

Liquid

存储溶液

PBS, 1% BSA, 20% Glycerol

存放说明

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

浓度

1 mg/ml (Please refer to the vial label for the specific concentration.)

免疫原种属

Human

免疫原

Recombinant protein encompassing a sequence within the center region of human TMS1. The exact sequence is proprietary.

纯化方式

Purified by antigen-affinity chromatography.

偶联

Unconjugated

RRID

AB_11165724

注意事项

仅供实验室使用。不适用于人类或动物的任何临床，治疗或诊断用途。不适合动物或人类食用。

相关讯息

别名

PYD and CARD domain containing , ASC , CARD5 , TMS , TMS-1 , TMS1

细胞定位

Cytoplasm

功能与背景

This gene encodes an adaptor protein that is composed of two protein-protein interaction domains: a N-terminal PYRIN-PAAD-DAPIN domain (PYD) and a C-terminal caspase-recruitment domain (CARD). The PYD and CARD domains are members of the six-helix bundle death domain-fold superfamily that mediates assembly of large signaling complexes in the inflammatory and apoptotic signaling pathways via the activation of caspase. In normal cells, this protein is localized to the cytoplasm; however, in cells undergoing apoptosis, it forms ball-like aggregates near the nuclear periphery. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq]

数据库

研究领域

產品图片

Anti-TMS1 antibody [N1C3] used in Western Blot (WB). GTX102474

GTX102474 WB Image

Overexpression

Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with TMS1 antibody [N1C3] (GTX102474) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-TMS1 antibody [N1C3] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX102474

GTX102474 ICC/IF Image

TMS1 antibody [N1C3] detects TMS1 protein at cytoplasm and nucleus by immunofluorescent analysis.
Sample: HCT 116 cells were fixed in 2% paraformaldehyde/culture medium at 37oC for 30 min.
Green: TMS1 protein stained by TMS1 antibody [N1C3] (GTX102474) diluted at 1:500.
Blue: Hoechst 33342 staining.
Scale bar = 10 μm.

Anti-TMS1 antibody [N1C3] used in Western Blot (WB). GTX102474

GTX102474 WB Image

Whole cell extract (30 μg) was separated by 12% SDS-PAGE, and the membrane was blotted with TMS1 antibody [N1C3] (GTX102474) diluted at 1:500.

文献引用

Application Reference

Zhang C et al. Mol Med Rep 2021; 23 (5) Cytokine secretion and pyroptosis of cholesteatoma keratinocytes mediated by AIM2 inflammasomes in response to cytoplasmic DNA.
实验应用 : WB, IHC-P
种属反应 : Human
Liu Y et al. Inflamm Res 2019; 68 (7) : 597-611 TNF-帢/calreticulin dual signaling induced NLRP3 inflammasome activation associated with HuR nucleocytoplasmic shuttling in rheumatoid arthritis.
实验应用 : IHC-Fr
种属反应 : Human
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SDS
PBS.pdf
Glycerol.pdf
Thimerosal.pdf
产品包装 报价 (¥)
¥ 4000
¥ 1700
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