Indirect ELISA analysis was performed by coating the plate with recombinant HEK293 expressed, mouse TNF-alpha Protein (209.42-3.27 nM). Coated protein was probed with TNF alpha antibody [HL1579] (GTX637058) (1 μg/mL). Goat anti-rabbit IgG antibody (HRP) (GTX213110-01) (1:10000) was used to detect the bound primary antibody.

Untreated (–) and treated (+) THP-1 whole cell extracts (30 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with TNF alpha antibody [HL1579] (GTX637058) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with TNF alpha antibody [HL1579] (GTX637058) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.