产品说明
摘要
TNF alpha antibody recognizes tumor necrosis factor alpha protein, a cytokine with a predicted molecular weight of 26 kDa. TNF alpha is mainly secreted by macrophages, but is also produced by various immune cells such as neutrophils, T helper cells, and NK cells. Dysregulation of TNF alpha expression is associated with various diseases including cancers and autoimmune disorders.
产品应用
应用说明
*最佳稀释倍数与浓度应由研究人员确认
| 实验应用 |
稀释倍数 |
| 1:500-1:3000 |
| 1:100-1:1000 |
| 1:100-1:1000 |
| Assay dependent |
WB: 免疫印迹
ICC/IF: 细胞染色
IHC-P: 石蜡切片
IHC-Fr: 冰冻切片
预期分子量
阳性对照
NCI-H929 , C8D30 , PC-12 , Rat-2 , Raji , TNF-transfected 293T
预测种属反应
Cat, Dog, Pig, Chimpanzee, Rhesus Monkey(>80% identity)
产品属性
形式
Liquid
存储溶液
PBS, 1% BSA, 20% Glycerol
存放说明
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
浓度
0.28 mg/ml (Please refer to the vial label for the specific concentration.)
免疫原种属
Human
免疫原
Full length human TNF alpha Recombinant protein.
纯化方式
Purified by antigen-affinity chromatography.
偶联
Unconjugated
RRID
AB_2885286
注意事项
仅供实验室使用。不适用于人类或动物的任何临床，治疗或诊断用途。不适合动物或人类食用。
相关讯息
别名
tumor necrosis factor , DIF , TNF-alpha , TNFA , TNFSF2 , TNLG1F
细胞定位
Cell membrane; Single-pass type II membrane protein , Tumor necrosis factor , soluble form: Secreted
功能与背景
This gene encodes a multifunctional proinflammatory cytokine that belongs to the tumor necrosis factor (TNF) superfamily. This cytokine is mainly secreted by macrophages. It can bind to, and thus functions through its receptors TNFRSF1A/TNFR1 and TNFRSF1B/TNFBR. This cytokine is involved in the regulation of a wide spectrum of biological processes including cell proliferation, differentiation, apoptosis, lipid metabolism, and coagulation. This cytokine has been implicated in a variety of diseases, including autoimmune diseases, insulin resistance, and cancer. Knockout studies in mice also suggested the neuroprotective function of this cytokine. [provided by RefSeq]
数据库
研究领域
產品图片
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GTX110520 WB Image
Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with TNF alpha antibody (GTX110520) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
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GTX110520 ICC/IF Image
TNF alpha antibody detects TNF alpha protein at membrane by immunofluorescent analysis.
Sample: jurkat cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: TNF alpha protein stained by TNF alpha antibody (GTX110520) diluted at 1:500.
Blue: Hoechst 33342 staining.
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GTX110520 WB Image
TNF alpha antibody detects TNF alpha protein by western blot analysis. Various whole cell extracts (30 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with TNF alpha antibody (GTX110520) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
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GTX110520 ICC/IF Image
TNF alpha antibody detects TNF alpha protein at cytoplasm by immunofluorescent analysis.
Sample: Raw264.7 cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: TNF alpha protein stained by TNF alpha antibody (GTX110520) diluted at 1:500.
Blue: Hoechst 33342 staining.
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GTX110520 WB Image
TNF alpha antibody detects TNF alpha protein by western blot analysis. Whole cell extracts (30 μg) was separated by 12% SDS-PAGE, and the membrane was blotted with TNF alpha antibody (GTX110520) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
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GTX110520 WB Image
Untreated (–) and treated (+) THP-1 whole cell extracts (30 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with TNF alpha antibody (GTX110520) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
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GTX110520 WB Image
Untreated (–) and treated (+) Raw264.7 whole cell extracts (30 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with TNF alpha antibody (GTX110520) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
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GTX110520 WB Image
The data was published in the 2022 in Int J Mol Sci. PMID: 35628464
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GTX110520 WB Image
The data was published in the 2022 in Int J Mol Sci. PMID: 35628464
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GTX110520 WB Image
The data was published in the 2022 in Int J Mol Sci. PMID: 35628464
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GTX110520 WB Image
The data was published in the journal Int J Mol Sci in 2019. PMID: 30987270
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GTX110520 WB Image
The data was published in the journal Front Neurol in 2017.PMID: 28713327
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GTX110520 WB Image
The data was published in the journal Cardiovasc Diabetol in 2014. PMID: 24666525
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GTX110520 IHC-P Image
The data was published in the 2022 in PLoS One. PMID: 35303036
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GTX110520 IHC-Fr Image
The data was published in the journal Nat Commun in 2019.PMID: 31757960
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GTX110520 WB Image
The data was published in the journal Nat Commun in 2019.PMID: 31757960
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GTX110520 IHC-P Image
The data was published in the journal Front Pharmacol in 2017.PMID: 29184499
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文献引用
产品评论
TNF alpha antibody
目录号 GTX110520
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Rating
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( Average 4 based on 1 users reviews)
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实验应用
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Western Blot(WB)
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( Average 4 based on 1 users reviews)
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Date :
Anonymous submitted on 22-Feb-2021
Application Tested :
WB
Sample Species :
Ms
Sample :
colonic mucosa
Amount used :
25 μg
Blocking :
5% fat dry milk in TBST, 25°C, 1Hr
Primary Antibody :
1:1000, 4°C, 16Hr
