产品应用
应用说明
*最佳稀释倍数与浓度应由研究人员确认
实验应用 |
稀释倍数 |
1:500-1:3000 |
1:100-1:1000 |
WB: 免疫印迹
ICC/IF: 细胞染色
IHC-P: 石蜡切片
IHC-Fr: 冰冻切片
预期分子量
阳性对照
HepG2 membrane extract , HepG2 , HepG2 membrane extract
预测种属反应
Mouse(>80% identity)
产品属性
形式
Liquid
存储溶液
PBS, 20% Glycerol
存放说明
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
浓度
1.38 mg/ml (Please refer to the vial label for the specific concentration.)
免疫原种属
Human
免疫原
Recombinant protein encompassing a sequence within the Intracellular domain of human TRPM2. The exact sequence is proprietary.
纯化方式
Purified by antigen-affinity chromatography.
偶联
Unconjugated
RRID
AB_1952408
注意事项
仅供实验室使用。不适用于人类或动物的任何临床,治疗或诊断用途。不适合动物或人类食用。
相关讯息
别名
transient receptor potential cation channel subfamily M member 2 , EREG1 , KNP3 , LTRPC2 , LTrpC-2 , NUDT9H , NUDT9L1 , TRPC7
细胞定位
Membrane; Multi-pass membrane protein
功能与背景
The protein encoded by this gene is a calcium-permeable cation channel that is regulated by free intracellular ADP-ribose. The encoded protein is activated by oxidative stress and confers susceptibility to cell death. Several alternatively spliced transcript variants of this gene have been described, but their full-length nature is not known. [provided by RefSeq]
数据库
研究领域
產品图片
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GTX112252 WB Image
HepG2 whole cell and membrane extracts (30 μg) were separated by 5% SDS-PAGE, and the membranes were blotted with TRPM2 antibody [N1N2-2], N-term (GTX112252) diluted at 1:500 and competitor's antibody (ACC-043) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
*The competitor is not affiliated with GeneTex and does not endorse this product.
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GTX112252 IHC-P Image
Immunohistochemical analysis of paraffin-embedded MDAMB468 xenograft, using TRPM2(GTX112252) antibody at 1:100 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min
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GTX112252 WB Image
HepG2 whole cell and membrane extracts (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with TRPM2 antibody [N1N2-2], N-term (GTX112252) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.
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GTX112252 IHC-P Image
TRPM2 antibody [N1N2-2], N-term detects TRPM2 protein at cytoplasm in rat liver by immunohistochemical analysis. Sample: Paraffin-embedded rat liver. TRPM2 antibody [N1N2-2], N-term (GTX112252) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
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文献引用
产品评论
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