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Thrombospondin 2抗体 [HL1194]

Recombinant antibody Orthogonal Overexpression
Anti-Thrombospondin 2 antibody [HL1194] used in Western Blot (WB). GTX636506
Anti-Thrombospondin 2 antibody [HL1194] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX636506
Anti-Thrombospondin 2 antibody [HL1194] used in Western Blot (WB). GTX636506
Anti-Thrombospondin 2 antibody [HL1194] used in ELISA (ELISA). GTX636506
Anti-Thrombospondin 2 antibody [HL1194] used in ELISA (ELISA). GTX636506

目录号 GTX636506

宿主

 Rabbit

克隆

 Monoclonal

克隆编号

 HL1194

同种型

 IgG

实验应用

 WB, ICC/IF, ELISA, Sandwich ELISA

种属反应

 Human
产品包装
100 μl (¥4000),
25 μl (¥1700)
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产品应用

应用说明

*最佳稀释倍数与浓度应由研究人员确认
实验应用 稀释倍数
WB Assay dependent
ICC/IF Assay dependent
ELISA Assay dependent
Sandwich ELISA Assay dependent

Note :

Sandwich ELISA
Capture : GTX636506, Detection : GTX636411 or Capture : GTX636411, Detection : GTX636506. Please notice that the detected antibody needs to be conjugated to HRP when paired with the capture antibody. Please contact us for custom HRP-conjugated antibody.
WB: 免疫印迹
ICC/IF: 细胞染色
IHC-P: 石蜡切片
IHC-Fr: 冰冻切片

预期分子量

130 kDa. ( Note )

预测种属反应

Bovine(>80% identity)

产品属性

形式

Liquid

存储溶液

PBS

存放说明

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

浓度

1 mg/ml (Please refer to the vial label for the specific concentration.)

免疫原种属

Human

免疫原

Recombinant protein encompassing a sequence within the center region of human Thrombospondin 2. The exact sequence is proprietary.

纯化方式

Affinity purified by Protein A.

偶联

Unconjugated

RRID

AB_2909996

注意事项

仅供实验室使用。不适用于人类或动物的任何临床，治疗或诊断用途。不适合动物或人类食用。

相关讯息

别名

thrombospondin 2 , TSP2

功能与背景

The protein encoded by this gene belongs to the thrombospondin family. It is a disulfide-linked homotrimeric glycoprotein that mediates cell-to-cell and cell-to-matrix interactions. This protein has been shown to function as a potent inhibitor of tumor growth and angiogenesis. Studies of the mouse counterpart suggest that this protein may modulate the cell surface properties of mesenchymal cells and be involved in cell adhesion and migration. [provided by RefSeq, Jul 2008]

数据库

研究领域

產品图片

Anti-Thrombospondin 2 antibody [HL1194] used in Western Blot (WB). GTX636506

GTX636506 WB Image

Overexpression

Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with Thrombospondin 2 antibody [HL1194] (GTX636506) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-Thrombospondin 2 antibody [HL1194] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX636506

GTX636506 ICC/IF Image

Thrombospondin 2 antibody [HL1194] detects Thrombospondin 2 protein at cytoplasm by immunofluorescent analysis.
Sample: Mock and transfected 293T cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: Thrombospondin 2 stained by Thrombospondin 2 antibody [HL1194] (GTX636506) diluted at 1:500.
Blue: Fluoroshield with DAPI (GTX30920).

Anti-Thrombospondin 2 antibody [HL1194] used in Western Blot (WB). GTX636506

GTX636506 WB Image

Orthogonal

AsPC-1 whole cell extract and conditioned medium (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with Thrombospondin 2 antibody [HL1194] (GTX636506) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-Thrombospondin 2 antibody [HL1194] used in ELISA (ELISA). GTX636506

GTX636506 ELISA Image

Sandwich ELISA detection of recombinant recombinant HEK293 expressed, full-length human thrombospondin 2 protein using antibodies as below.

Capture: Thrombospondin 2 antibody [HL1140] (GTX636411) (5 μg/mL)
Detection: HRP-conjugated Thrombospondin 2 antibody [HL1194] (GTX636506) (1 μg/mL)
Please notice that GTX636506 needs to be conjugated to HRP to function as the detection antibody when paired with GTX636411. Please contact us for custom HRP-conjugated antibody.

Anti-Thrombospondin 2 antibody [HL1194] used in ELISA (ELISA). GTX636506

GTX636506 ELISA Image

Sandwich ELISA detection of recombinant human thrombospondin 2 protein using antibodies as below.

Capture: Thrombospondin 2 antibody [HL1194] (GTX636506) (5 μg/mL)
Detection: HRP-conjugated Thrombospondin 2 antibody [HL1140] (GTX636411) (1 μg/mL)
Please notice that GTX636411 needs to be conjugated to HRP to function as the detection antibody when paired with GTX636506. Please contact us for custom HRP-conjugated antibody.

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产品包装 报价 (¥)
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