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Transgelin 2抗体 [N1C3]

KO/KD
Anti-Transgelin 2 antibody [N1C3] used in Western Blot (WB). GTX115082
Anti-Transgelin 2 antibody [N1C3] used in Western Blot (WB). GTX115082
Anti-Transgelin 2 antibody [N1C3] used in IHC (Paraffin sections) (IHC-P). GTX115082
Anti-Transgelin 2 antibody [N1C3] used in Western Blot (WB). GTX115082
Anti-Transgelin 2 antibody [N1C3] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX115082
Anti-Transgelin 2 antibody [N1C3] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX115082
Anti-Transgelin 2 antibody [N1C3] used in IHC (Paraffin sections) (IHC-P). GTX115082

目录号 GTX115082

文献引用 6

宿主

 Rabbit

克隆

 Polyclonal

同种型

 IgG

实验应用

 WB, ICC/IF, IHC-P

种属反应

 Human, Mouse
产品包装
100 μl (¥4000),
25 μl (¥1700)
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产品应用

应用说明

*最佳稀释倍数与浓度应由研究人员确认
实验应用 稀释倍数
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
WB: 免疫印迹
ICC/IF: 细胞染色
IHC-P: 石蜡切片
IHC-Fr: 冰冻切片

预期分子量

22 kDa. ( Note )

阳性对照

Mouse brain , 293T , A431

预测种属反应

Rat, Bovine, Rhesus Monkey(>80% identity)

产品属性

形式

Liquid

存储溶液

PBS, 1% BSA, 20% Glycerol

存放说明

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

浓度

0.53 mg/ml (Please refer to the vial label for the specific concentration.)

免疫原种属

Human

免疫原

Recombinant protein encompassing a sequence within the center region of human Transgelin 2. The exact sequence is proprietary.

纯化方式

Purified by antigen-affinity chromatography.

偶联

Unconjugated

RRID

AB_10619797

注意事项

仅供实验室使用。不适用于人类或动物的任何临床，治疗或诊断用途。不适合动物或人类食用。

相关讯息

别名

transgelin 2 , HA1756

功能与背景

The protein encoded by this gene is a homolog of the protein transgelin, which is one of the earliest markers of differentiated smooth muscle. The function of this protein has not yet been determined. [provided by RefSeq]

数据库

研究领域

產品图片

Anti-Transgelin 2 antibody [N1C3] used in Western Blot (WB). GTX115082

GTX115082 WB Image

KO/KD

Wild-type (WT) and Transgelin 2 knockout (KO) 293T cell extracts (30 μg) were separated by 15% SDS-PAGE, and the membrane was blotted with Transgelin 2 antibody [N1C3] (GTX115082) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-Transgelin 2 antibody [N1C3] used in Western Blot (WB). GTX115082

GTX115082 WB Image

Sample (50 μg of whole cell lysate)
A: mouse brain
12% SDS PAGE
GTX115082 diluted at 1:1000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-Transgelin 2 antibody [N1C3] used in IHC (Paraffin sections) (IHC-P). GTX115082

GTX115082 IHC-P Image

Transgelin 2 antibody [N1C3] detects Transgelin 2 protein at cytoplasm in mouse prostate by immunohistochemical analysis.
Sample: Paraffin-embedded mouse prostate.
Transgelin 2 antibody [N1C3] (GTX115082) diluted at 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-Transgelin 2 antibody [N1C3] used in Western Blot (WB). GTX115082

GTX115082 WB Image

Transgelin 2 antibody detects Transgelin 2 protein by western blot analysis. Various whole cell extracts (30 μg) were separated by 15% SDS-PAGE, and the membrane was blotted with Transgelin 2 antibody (GTX115082) diluted by 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-Transgelin 2 antibody [N1C3] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX115082

GTX115082 ICC/IF Image

Transgelin 2 antibody [N1C3] detects Transgelin 2 protein at cytoplasm and nucleus by immunofluorescent analysis.
Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: Transgelin 2 protein stained by Transgelin 2 antibody [N1C3] (GTX115082) diluted at 1:500.
Red: phalloidin, a cytoskeleton marker, stained by phalloidin (invitrogen, A12380) diluted at 1:200.
Blue: Hoechst 33342 staining.
Anti-Transgelin 2 antibody [N1C3] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX115082

GTX115082 ICC/IF Image

Transgelin 2 antibody [N1C3] detects Transgelin 2 protein at cytoplasm and nucleus by immunofluorescent analysis.
Sample: A549 cells were fixed in ice-cold MeOH for 5 min.
Green: Transgelin 2 protein stained by Transgelin 2 antibody [N1C3] (GTX115082) diluted at 1:500.
Blue: Hoechst 33342 staining.
Scale bar = 10 μm.

Anti-Transgelin 2 antibody [N1C3] used in IHC (Paraffin sections) (IHC-P). GTX115082

GTX115082 IHC-P Image

Transgelin 2 antibody [N1C3] detects Transgelin 2 protein at cytoplasm in mouse pancreas by immunohistochemical analysis.
Sample: Paraffin-embedded mouse pancreas.
Transgelin 2 antibody [N1C3] (GTX115082) diluted at 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

文献引用

Application Reference

Girard CA et al. Cancer Res 2020; 80 (10) : 1927-1941 A Feed-Forward Mechanosignaling Loop Confers Resistance to Therapies Targeting the MAPK Pathway in BRAF-Mutant Melanoma.
实验应用 : WB
种属反应 : Human
Liu L et al. Mol Cancer Ther 2019; (Epub)
Zheng X et al. Int J Oncol 2017; 50 (3) : 893-902 SB-T-121205, a next-generation taxane, enhances apoptosis and inhibits migration/invasion in MCF-7/PTX cells.
Jiangxia Cai et al. Phytomedicine 2014; 21 (12) salvianolic acid a reverses * resistance in human breast cancer mcf-7 cells via targeting the expression of transgelin 2 and attenuating pi3 k/akt pathway
实验应用 : WB
种属反应 : Human
Cai J et al. Phytomedicine 2014; paeonol reverses * resistance in human breast cancer cells by regulating the expression of transgelin 2.
实验应用 : WB
种属反应 : Human
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SDS
PBS.pdf
Glycerol.pdf
Thimerosal.pdf
产品包装 报价 (¥)
¥ 4000
¥ 1700
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