产品说明
摘要
Phosphate-Buffered Saline with Tween Solution (10X)
产品应用
应用说明
Dilute this 10X buffer 10 times (e.g. 90 ml of deionized or distilled water + 10 ml of this buffer), mix well, and store 1 X buffer solution at room temperature or 2-8ºC for several weeks. Wash membrane or ELISA plates for 10 minutes each for three times. For 10x10 cm membrane use at least 50 ml buffer with shaking gently. This buffer is used in between steps in immunoassays protocol. For diluting blocking solution, primary antibody or HRP-secondary antibody, please refer to WB or ELISA protocol.
产品属性
形式
Liquid
存放说明
Room temperature. If stored at 2-8ºC, some sodium chloride will be precipitated, if this occurs, bring the buffer to room temperature and mix well till all sodium chloride is dissolved.
注意事项
仅供实验室使用。不适用于人类或动物的任何临床，治疗或诊断用途。不适合动物或人类食用。
相关讯息
功能与背景
This buffer is supplied as 10X PBS solution containing 1% Tween. It is used as 1X in all western blotting, ELISA and Chemiluminescence assays as a washing buffer, making blocking solution, diluting HRP-secondary and primary antibody etc. This buffer does not contain any sodium azide or mercury preservative and can be used for dilution of all antibodies, including peroxidase. This buffer does not contain any proteins.
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