摘要
The Prestained Protein Ladder is a three-color protein standard with 12 pre-stained proteins covering a wide range molecular weights from 10 to 245 kDa. Proteins are covalently coupled with a blue chromophore except for two reference bands (one green and one red band at 25 kDa and 75 kDa respectively) when separated on SDS-PAGE (Tris-glycine buffer). The Prestained Protein Ladder is designed for monitoring protein separation during SDS-polyacrylamide gel electrophoresis, verification of Western transfer efficiency on membranes (PVDF, nylon, or nitrocellulose) and for approximating the size of proteins. The ladder is supplied in gel loading buffer and is ready to use.
形式
Liquid
存储溶液
20mM Tris-Phosphate pH7.5 at 25ºC), 2 % SDS, 1mM Dithiothreitol, 3.6M Urea, 15 % (v/v) Glycerol
保存剂
No preservative
存放说明
Store at 4ºC short term (3 months). For extended storage aliquot and store at -20ºC or below. Avoid freeze-thaw cycles.
注意事项
仅供实验室使用。不适用于人类或动物的任何临床,治疗或诊断用途。不适合动物或人类食用。