产品应用
应用说明
*最佳稀释倍数与浓度应由研究人员确认
| 实验应用 |
稀释倍数 |
| 1:500-1:3000 |
| Assay dependent |
| Assay dependent |
WB: 免疫印迹
ICC/IF: 细胞染色
IHC-P: 石蜡切片
IHC-Fr: 冰冻切片
预期分子量
产品属性
形式
Liquid
存储溶液
PBS
存放说明
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
浓度
1 mg/ml (Please refer to the vial label for the specific concentration.)
免疫原种属
Human
免疫原
Recombinant fragmemt of human Tryptophanyl tRNA synthetase
纯化方式
Affinity purified by Protein A.
偶联
Unconjugated
注意事项
仅供实验室使用。不适用于人类或动物的任何临床，治疗或诊断用途。不适合动物或人类食用。
相关讯息
别名
tryptophanyl-tRNA synthetase , GAMMA-2 , HMN9 , IFI53 , IFP53
细胞定位
Cytoplasm
功能与背景
Aminoacyl-tRNA synthetases catalyze the aminoacylation of tRNA by their cognate amino acid. Because of their central role in linking amino acids with nucleotide triplets contained in tRNAs, aminoacyl-tRNA synthetases are thought to be among the first proteins that appeared in evolution. Two forms of tryptophanyl-tRNA synthetase exist, a cytoplasmic form, named WARS, and a mitochondrial form, named WARS2. Tryptophanyl-tRNA synthetase (WARS) catalyzes the aminoacylation of tRNA(trp) with tryptophan and is induced by interferon. Tryptophanyl-tRNA synthetase belongs to the class I tRNA synthetase family. Four transcript variants encoding two different isoforms have been found for this gene. [provided by RefSeq, Jul 2008]
数据库
研究领域
產品图片
![Anti-Tryptophanyl tRNA synthetase antibody [HL2186] used in Western Blot (WB). GTX638190](/templates/default/js/lightbox/images/loading.gif "Anti-Tryptophanyl tRNA synthetase antibody [HL2186] used in Western Blot (WB). GTX638190")
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GTX638190 WB Image
Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with Tryptophanyl tRNA synthetase antibody [HL2186] (GTX638190) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
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GTX638190 WB Image
Various whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with Tryptophanyl tRNA synthetase antibody [HL2186] (GTX638190) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody. Corresponding RNA expression data for the same cell lines are based on Human Protein Atlas program.
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GTX638190 WB Image
Mouse tissue extract (50 μg) was separated by 10% SDS-PAGE, and the membrane was blotted with Tryptophanyl tRNA synthetase antibody [HL2186] (GTX638190) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
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GTX638190 IHC-P Image
Tryptophanyl tRNA synthetase antibody [HL2186] detects Tryptophanyl tRNA synthetase protein at cell membrane and secreted by immunohistochemical analysis.Sample: Paraffin-embedded rat kidney.Tryptophanyl tRNA synthetase stained by Tryptophanyl tRNA synthetase antibody [HL2186] (GTX638190) diluted at 1:100.Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
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GTX638190 IHC-P Image
Tryptophanyl tRNA synthetase antibody [HL2186] detects Tryptophanyl tRNA synthetase protein at cell membrane and secreted by immunohistochemical analysis.Sample: Paraffin-embedded mouse adrenal gland.Tryptophanyl tRNA synthetase stained by Tryptophanyl tRNA synthetase antibody [HL2186] (GTX638190) diluted at 1:100.Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
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GTX638190 ICC/IF Image
Tryptophanyl tRNA synthetase antibody [HL2186] detects Tryptophanyl tRNA synthetase protein at cytoplasm by immunofluorescent analysis.Sample: A431 cells were fixed in ice-cold MeOH for 5 min.Green: Tryptophanyl tRNA synthetase stained by Tryptophanyl tRNA synthetase antibody [HL2186] (GTX638190) diluted at 1:500.Blue: Fluoroshield with DAPI (GTX30920).
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GTX638190 WB Image
Various whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with Tryptophanyl tRNA synthetase antibody [HL2186] (GTX638190) diluted at 1:1000. The HRP-conjated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody. Corresponding RNA expression data for the same cell lines are based on Human Protein Atlas program.
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GTX638190 WB Image
Whole zebrafish extract (30 μg) was separated by 10% SDS-PAGE, and the membrane was blotted with Tryptophanyl tRNA synthetase antibody [HL2186] (GTX638190) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
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文献引用
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产品评论
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![Anti-Tryptophanyl tRNA synthetase antibody [HL2186] used in Western Blot (WB). GTX638190](/upload/website/prouct_img/small/GTX638190/GTX638190_T-44935_20230203_WB_shRNA_watermark_23020621_983.webp "Anti-Tryptophanyl tRNA synthetase antibody [HL2186] used in Western Blot (WB). GTX638190")
![Anti-Tryptophanyl tRNA synthetase antibody [HL2186] used in Western Blot (WB). GTX638190](/upload/website/prouct_img/small/GTX638190/GTX638190_T-44935_20230203_WB_TPM_watermark_23020621_688.webp "Anti-Tryptophanyl tRNA synthetase antibody [HL2186] used in Western Blot (WB). GTX638190")
![Anti-Tryptophanyl tRNA synthetase antibody [HL2186] used in Western Blot (WB). GTX638190](/upload/website/prouct_img/small/GTX638190/GTX638190_T-44935_20230203_WB_M_lung_23020621_966.webp "Anti-Tryptophanyl tRNA synthetase antibody [HL2186] used in Western Blot (WB). GTX638190")
![Anti-Tryptophanyl tRNA synthetase antibody [HL2186] used in IHC (Paraffin sections) (IHC-P). GTX638190](/upload/website/prouct_img/small/GTX638190/GTX638190_T-44935_20230217_IHC-P_R_23030219_439.webp "Anti-Tryptophanyl tRNA synthetase antibody [HL2186] used in IHC (Paraffin sections) (IHC-P). GTX638190")
![Anti-Tryptophanyl tRNA synthetase antibody [HL2186] used in IHC (Paraffin sections) (IHC-P). GTX638190](/upload/website/prouct_img/small/GTX638190/GTX638190_T-44935_20230217_IHC-P_M_23030219_688.webp "Anti-Tryptophanyl tRNA synthetase antibody [HL2186] used in IHC (Paraffin sections) (IHC-P). GTX638190")
![Anti-Tryptophanyl tRNA synthetase antibody [HL2186] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX638190](/upload/website/prouct_img/small/GTX638190/GTX638190_45005_20230512_ICC_IF_23060622_371.webp "Anti-Tryptophanyl tRNA synthetase antibody [HL2186] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX638190")
![Anti-Tryptophanyl tRNA synthetase antibody [HL2186] used in Western Blot (WB). GTX638190](/upload/website/prouct_img/small/GTX638190/GTX638190_45005_20230414_WB_TPM_watermark_23041723_360.webp "Anti-Tryptophanyl tRNA synthetase antibody [HL2186] used in Western Blot (WB). GTX638190")
![Anti-Tryptophanyl tRNA synthetase antibody [HL2186] used in Western Blot (WB). GTX638190](/upload/website/prouct_img/small/GTX638190/GTX638190_45005_20230609_WB_Z_23061400_423.webp "Anti-Tryptophanyl tRNA synthetase antibody [HL2186] used in Western Blot (WB). GTX638190")
![Anti-Tryptophanyl tRNA synthetase antibody [HL2186] used in Western Blot (WB). GTX638190](/upload/website/prouct_img/normal/GTX638190/GTX638190_T-44935_20230203_WB_shRNA_watermark_23020621_983.webp "Anti-Tryptophanyl tRNA synthetase antibody [HL2186] used in Western Blot (WB). GTX638190")
![Anti-Tryptophanyl tRNA synthetase antibody [HL2186] used in Western Blot (WB). GTX638190](/upload/website/prouct_img/normal/GTX638190/GTX638190_T-44935_20230203_WB_TPM_watermark_23020621_688.webp "Anti-Tryptophanyl tRNA synthetase antibody [HL2186] used in Western Blot (WB). GTX638190")
![Anti-Tryptophanyl tRNA synthetase antibody [HL2186] used in Western Blot (WB). GTX638190](/upload/website/prouct_img/normal/GTX638190/GTX638190_T-44935_20230203_WB_M_lung_23020621_966.webp "Anti-Tryptophanyl tRNA synthetase antibody [HL2186] used in Western Blot (WB). GTX638190")
![Anti-Tryptophanyl tRNA synthetase antibody [HL2186] used in IHC (Paraffin sections) (IHC-P). GTX638190](/upload/website/prouct_img/normal/GTX638190/GTX638190_T-44935_20230217_IHC-P_R_23030219_439.webp "Anti-Tryptophanyl tRNA synthetase antibody [HL2186] used in IHC (Paraffin sections) (IHC-P). GTX638190")
![Anti-Tryptophanyl tRNA synthetase antibody [HL2186] used in IHC (Paraffin sections) (IHC-P). GTX638190](/upload/website/prouct_img/normal/GTX638190/GTX638190_T-44935_20230217_IHC-P_M_23030219_688.webp "Anti-Tryptophanyl tRNA synthetase antibody [HL2186] used in IHC (Paraffin sections) (IHC-P). GTX638190")
![Anti-Tryptophanyl tRNA synthetase antibody [HL2186] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX638190](/upload/website/prouct_img/normal/GTX638190/GTX638190_45005_20230512_ICC_IF_23060622_371.webp "Anti-Tryptophanyl tRNA synthetase antibody [HL2186] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX638190")
![Anti-Tryptophanyl tRNA synthetase antibody [HL2186] used in Western Blot (WB). GTX638190](/upload/website/prouct_img/normal/GTX638190/GTX638190_45005_20230414_WB_TPM_watermark_23041723_360.webp "Anti-Tryptophanyl tRNA synthetase antibody [HL2186] used in Western Blot (WB). GTX638190")
![Anti-Tryptophanyl tRNA synthetase antibody [HL2186] used in Western Blot (WB). GTX638190](/upload/website/prouct_img/normal/GTX638190/GTX638190_45005_20230609_WB_Z_23061400_423.webp "Anti-Tryptophanyl tRNA synthetase antibody [HL2186] used in Western Blot (WB). GTX638190")
