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Twist1/2抗体

Anti-Twist1/2 antibody used in Western Blot (WB). GTX127310
Anti-Twist1/2 antibody used in IHC (Paraffin sections) (IHC-P). GTX127310
Anti-Twist1/2 antibody used in IHC (Paraffin sections) (IHC-P). GTX127310
Anti-Twist1/2 antibody used in Western Blot (WB). GTX127310
Anti-Twist1/2 antibody used in IHC (Paraffin sections) (IHC-P). GTX127310
Anti-Twist1/2 antibody used in Western Blot (WB). GTX127310
Anti-Twist1/2 antibody used in Western Blot (WB). GTX127310
Anti-Twist1/2 antibody used in Western Blot (WB). GTX127310
Anti-Twist1/2 antibody used in Western Blot (WB). GTX127310
Anti-Twist1/2 antibody used in Western Blot (WB). GTX127310
Anti-Twist1/2 antibody used in Western Blot (WB). GTX127310
Anti-Twist1/2 antibody used in Western Blot (WB). GTX127310
Anti-Twist1/2 antibody used in Western Blot (WB). GTX127310
Anti-Twist1/2 antibody used in Western Blot (WB). GTX127310
Anti-Twist1/2 antibody used in Western Blot (WB). GTX127310
Anti-Twist1/2 antibody used in Immunohistochemistry (IHC). GTX127310

目录号 GTX127310

宿主

Rabbit

克隆

Polyclonal

同种型

IgG

实验应用

WB, IHC-P, IHC

种属反应

Human, Mouse, Rat, Drosophila
产品包装
100 μl (¥4000),
25 μl (¥1700)

产品应用

应用说明

*最佳稀释倍数与浓度应由研究人员确认
实验应用 稀释倍数
WB 1:500-1:10000
IHC-P 1:100-1:1000
IHC Assay dependent
WB: 免疫印迹
ICC/IF: 细胞染色
IHC-P: 石蜡切片
IHC-Fr: 冰冻切片

预期分子量

21 kDa. ( Note )

阳性对照

Jurkat , Raji , THP-1 , HL-60 , human Twist1-transfected 293T

预测种属反应

Bovine, Chicken, Xenopus tropicalis, Chimpanzee(>80% identity)

产品属性

形式

Liquid

存储溶液

PBS, 20% Glycerol

存放说明

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

浓度

0.67 mg/ml (Please refer to the vial label for the specific concentration.)

免疫原种属

Human

免疫原

Recombinant protein encompassing a sequence within the center region of human Twist1/2. The exact sequence is proprietary.

纯化方式

Purified by antigen-affinity chromatography.

偶联

Unconjugated

RRID

AB_2885640

注意事项

仅供实验室使用。不适用于人类或动物的任何临床,治疗或诊断用途。不适合动物或人类食用。

相关讯息

别名

ACS3 antibody , B-HLH DNA binding protein antibody , BPES2 antibody , BPES3 antibody , CRS1 antibody , DERMO1 antibody , H-twist antibody , MGC117334 antibody , SCS antibody , TWIST antibody , TWIST homolog of drosophila antibody , TWIST1 antibody , TWIST2 antibody , bHLHa38 antibody , bHLHa39 antibody , class A basic helix-loop-helix protein 38 antibody , class A basic helix-loop-helix protein 39 antibody , dermis-expressed protein 1 antibody , twist homolog 2 (Drosophila) antibody , twist-related bHLH protein Dermo1 antibody , twist-related protein 1 antibody , twist-related protein 2 antibody

细胞定位

Nucleus

功能与背景

This gene encodes a basic helix-loop-helix (bHLH) transcription factor that plays an important role in embryonic development. The encoded protein forms both homodimers and heterodimers that bind to DNA E box sequences and regulate the transcription of genes involved in cranial suture closure during skull development. This protein may also regulate neural tube closure, limb development and brown fat metabolism. This gene is hypermethylated and overexpressed in multiple human cancers, and the encoded protein promotes tumor cell invasion and metastasis. Mutations in this gene cause Saethre-Chotzen syndrome in human patients, which is characterized by craniosynostosis, ptosis and hypertelorism. [provided by RefSeq, Aug 2017]

研究领域

產品图片

Anti-Twist1/2 antibody used in Western Blot (WB). GTX127310

GTX127310 WB Image

Various tissue extracts (50 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with Twist1/2 antibody (GTX127310) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-Twist1/2 antibody used in IHC (Paraffin sections) (IHC-P). GTX127310

GTX127310 IHC-P Image

Twist1/2 antibody detects Twist1/2 protein at nucleus on mouse prostate by immunohistochemical analysis.
Sample: Paraffin-embedded mouse prostate.
Twist1/2 antibody (GTX127310) dilution: 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-Twist1/2 antibody used in IHC (Paraffin sections) (IHC-P). GTX127310

GTX127310 IHC-P Image

Twist1/2 antibody detects Twist1/2 protein at nucleus on rat fore brain by immunohistochemical analysis.
Sample: Paraffin-embedded rat fore brain.
Twist1/2 antibody (GTX127310) dilution: 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-Twist1/2 antibody used in Western Blot (WB). GTX127310

GTX127310 WB Image

Twist1/2 antibody detects Twist1 protein by western blot analysis. Non-transfected (-) and Twist1-transfected (+) 293T whole cell extracts (30 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with Twist1/2 antibody (GTX127310) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-Twist1/2 antibody used in IHC (Paraffin sections) (IHC-P). GTX127310

GTX127310 IHC-P Image

Twist1/2 antibody detects Twist1/2 protein at nucleus on mouse liver by immunohistochemical analysis.
Sample: Paraffin-embedded mouse liver.
Twist1/2 antibody (GTX127310) dilution: 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-Twist1/2 antibody used in Western Blot (WB). GTX127310

GTX127310 WB Image

Twist1/2 antibody detects Twist1/2 protein by western blot analysis. Various whole cell extracts (30 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with Twist1/2 antibody (GTX127310) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-Twist1/2 antibody used in Western Blot (WB). GTX127310

GTX127310 WB Image

Various whole cell extracts (30 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with Twist1/2 antibody (GTX127310) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-Twist1/2 antibody used in Western Blot (WB). GTX127310

GTX127310 WB Image

The data was published in the journal Oncotarget in 2016.PMID: 26840566

Anti-Twist1/2 antibody used in Western Blot (WB). GTX127310

GTX127310 WB Image

The data was published in the journal Sci Rep in 2017. PMID: 28134285

Anti-Twist1/2 antibody used in Western Blot (WB). GTX127310

GTX127310 WB Image

The data was published in the journal Biomolecules in 2019.PMID: 31877715

Anti-Twist1/2 antibody used in Western Blot (WB). GTX127310

GTX127310 WB Image

The data was published in the journal Sci Rep in 2019.PMID: 30816208

Anti-Twist1/2 antibody used in Western Blot (WB). GTX127310

GTX127310 WB Image

The data was published in the journal Sci Rep in 2019.PMID: 30816208

Anti-Twist1/2 antibody used in Western Blot (WB). GTX127310

GTX127310 WB Image

The data was published in the journal Cell Death Dis in 2015. PMID: 26136074

Anti-Twist1/2 antibody used in Western Blot (WB). GTX127310

GTX127310 WB Image

The data was published in the journal PLoS One in 2017. PMID: 29182685

Anti-Twist1/2 antibody used in Western Blot (WB). GTX127310

GTX127310 WB Image

The data was published in the journal Front Oncol in 2019. PMID: 31440468

Anti-Twist1/2 antibody used in Immunohistochemistry (IHC). GTX127310

GTX127310 IHC Image

The data was published in the journal Sci Adv in 2019.PMID: 31309148

文献引用

产品评论

Twist1/2 antibody 目录号 GTX127310

Rating ( Average 4.7 based on 12 users reviews)
实验应用
Western Blot(WB) ( Average 4.7 based on 12 users reviews)
Date : Anonymous submitted on 02-May-2023
Score :
Application Tested : WB
Sample Species : Hu
Sample : MDA-MB-231 cell lysates with/without treatment
Amount used : 40μg
Blocking : 5% non-fat milk in TBST, 25°C, 1Hr
Primary Antibody : 1:1000, 4°C, 16Hr
Date : Anonymous submitted on 08-Aug-2022
Score :
Application Tested : WB
Sample Species : Hu
Sample : Huh7 cells
Amount used : 30μg
Blocking : 5% non-fat milk, BSA in TBST, 4°C, 1Hr
Primary Antibody : 1:1000, 4°C, > 12Hr
Date : Anonymous submitted on 19-Apr-2022
Score :
Application Tested : WB
Sample Species : Hu
Sample : HSC-3 cells with/without drug treatment
Amount used : 30μg
Blocking : 5% non-fat milk in TBST, 30°C, 1Hr
Primary Antibody : 1:1000, 4°C, 18Hr
Date : Anonymous submitted on 01-Nov-2017
Score :
Application Tested : WB
Sample Species : Hu
Sample : KLE cells
Amount used : 10 μg
Blocking : PVDF Blocking Reagent for Can Get Signal, 25°C, 1Hr
Primary Antibody : 1:1000, 4°C, 24Hr
条带说明 : Lane 1: Control, Lane 2: TGF-β 5ng/mL treatment
Date : Anonymous submitted on 18-Jul-2016
Score :
Application Tested : WB
Sample Species : Hu
Sample : A549 cells
Amount used : 1* 10^5 cells
Blocking : 5% milk, 25°C, 1Hr
Primary Antibody : 1:1000, 4°C, 16Hr
Date : Anonymous submitted on 29-Apr-2016
Score :
Application Tested : WB
Sample Species : Hu
Sample : PC3 cells
Amount used : 30μg
Blocking : 5% milk in PBST, 25°C, 1Hr
Primary Antibody : 1:500, 4°C, 24Hr
Date : Anonymous submitted on 24-Jul-2015
Score :
Application Tested : WB
Sample Species : Hu
Sample : MDA-MB-231 cells
Amount used : 30μg
Blocking : 5% milk, 25°C, 1Hr
Primary Antibody : 1:1000, 4°C, 24Hr
Date : Anonymous submitted on 04-Aug-2014
Score :
Application Tested : WB
Sample Species : Hu
Sample : RL95-2 cells
Amount used : 120μg
Blocking : 5% milk in TBST, 25°C, 1Hr
Primary Antibody : 1:1000, 4°C, 16Hr
Date : Anonymous submitted on 07-Apr-2014
Score :
Application Tested : WB
Sample Species : Hu
Sample : Toledo cells, SU-DHL-5 cells, HT cells, DB cells, transfected hTWIST1 in HEK293 cells
Amount used : 30μg
Blocking : 5% milk in TBST, 25°C, 1Hr
Primary Antibody : 1:2000, 4°C, 16Hr
Date : Anonymous submitted on 11-Jun-2013
Score :
Application Tested : WB
Sample Species : Hu
Sample : PC9 cells
Amount used : 30μg
Blocking : 5% milk, 25°C, 1Hr
Primary Antibody : 1:4000, 25°C, 4Hr
Date : Anonymous submitted on 22-Nov-2012
Score :
Application Tested : WB
Sample Species : Hu
Sample : CL1-5 cells
Amount used : 40μg
Blocking : 5% milk, 25°C, 3Hr
Primary Antibody : 1:1000, 4°C, 16Hr
Date : Anonymous submitted on 31-Oct-2012
Score :
Application Tested : WB
Sample Species : Hu
Sample : OECM1 cells
Amount used : 40μg
Blocking : 5% milk in TBST, 25°C, 1Hr
Primary Antibody : 1:1000, 4°C, 16Hr
Application Reference
Esparza-L?pez J et al. Oncol Lett 2022; 23 (2):50
实验应用 : WB
种属反应 : Human
SDS
PBS.pdf
Glycerol.pdf
Thimerosal.pdf
产品包装 报价 (¥)
¥ 4000
¥ 1700
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