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Tyrosine Hydroxylase抗体 [GT234]

Anti-Tyrosine Hydroxylase antibody [GT234] used in IHC (Paraffin sections) (IHC-P). GTX634481
Anti-Tyrosine Hydroxylase antibody [GT234] used in Western Blot (WB). GTX634481
Anti-Tyrosine Hydroxylase antibody [GT234] used in Western Blot (WB). GTX634481
Anti-Tyrosine Hydroxylase antibody [GT234] used in IHC (Paraffin sections) (IHC-P). GTX634481

目录号 GTX634481

文献引用 2
1

宿主

 Mouse

克隆

 Monoclonal

克隆编号

 GT234

同种型

 IgG1

实验应用

 WB, ICC/IF, IHC-P

种属反应

 Mouse, Rat
产品包装
100 μl (¥4000),
25 μl (¥1700)
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产品应用

应用说明

*最佳稀释倍数与浓度应由研究人员确认
实验应用 稀释倍数
WB 1:500-1:3000
ICC/IF Assay dependent
IHC-P 1:100-1:1000
WB: 免疫印迹
ICC/IF: 细胞染色
IHC-P: 石蜡切片
IHC-Fr: 冰冻切片

预期分子量

59 kDa. ( Note )

阳性对照

rat brain , mouse brain

预测种属反应

Human, Zebrafish, Bovine, Dog, Chicken, Japanese Medaka(>80% identity)

产品属性

形式

Liquid

存储溶液

PBS

存放说明

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

浓度

1 mg/ml (Please refer to the vial label for the specific concentration.)

免疫原种属

Human

免疫原

Recombinant protein encompassing a sequence within the center region of human Tyrosine Hydroxylase. The exact sequence is proprietary.

纯化方式

Affinity purified by Protein G.

偶联

Unconjugated

RRID

AB_2802158

注意事项

仅供实验室使用。不适用于人类或动物的任何临床，治疗或诊断用途。不适合动物或人类食用。

相关讯息

别名

tyrosine hydroxylase , DYT14 , DYT5b , TYH

功能与背景

The protein encoded by this gene is involved in the conversion of tyrosine to dopamine. It is the rate-limiting enzyme in the synthesis of catecholamines, hence plays a key role in the physiology of adrenergic neurons. Mutations in this gene have been associated with autosomal recessive Segawa syndrome. Alternatively spliced transcript variants encoding different isoforms have been noted for this gene. [provided by RefSeq, Jul 2008]

数据库

研究领域

產品图片

Anti-Tyrosine Hydroxylase antibody [GT234] used in IHC (Paraffin sections) (IHC-P). GTX634481

GTX634481 IHC-P Image

Tyrosine Hydroxylase antibody [GT234] detects Tyrosine Hydroxylase protein in midbrain dopaminergic neurons by immunohistochemical analysis.
Sample: Paraffin-embedded mouse brain.
Green: Tyrosine Hydroxylase stained by Tyrosine Hydroxylase antibody [GT234] (GTX634481) diluted at 1:250.
Blue: Fluoroshield with DAPI (GTX30920).
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-Tyrosine Hydroxylase antibody [GT234] used in Western Blot (WB). GTX634481

GTX634481 WB Image

Mouse tissue extract (30 μg) was separated by 10% SDS-PAGE, and the membrane was blotted with Tyrosine Hydroxylase antibody [GT234] (GTX634481) diluted at 1:1000. The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody.

Anti-Tyrosine Hydroxylase antibody [GT234] used in Western Blot (WB). GTX634481

GTX634481 WB Image

Rat tissue extract (50 μg) was separated by 7.5% SDS-PAGE, and the membrane was blotted with Tyrosine Hydroxylase antibody [GT234] (GTX634481) diluted at 1:1000. The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody.

Anti-Tyrosine Hydroxylase antibody [GT234] used in IHC (Paraffin sections) (IHC-P). GTX634481

GTX634481 IHC-P Image

Tyrosine Hydroxylase antibody [GT234] detects Tyrosine Hydroxylase protein at cytoplasm of dopaminergic neurons by immunohistochemical analysis.
Sample: Paraffin-embedded mouse midbrain.
Green: Tyrosine Hydroxylase stained by Tyrosine Hydroxylase antibody [GT234] (GTX634481) diluted at 1:250.
Blue: Fluoroshield with DAPI (GTX30920).
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

文献引用

Application Reference

Zhou R et al. Cell Prolif 2024; 57 (1) : e13520 Decreased YB-1 expression denervates brown adipose tissue and contributes to age-related metabolic dysfunction.
Feng X et al. Nat Commun 2023; 14 (1) : 3208 Senescent immune cells accumulation promotes brown adipose tissue dysfunction during aging.
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产品评论

Tyrosine Hydroxylase antibody [GT234] 目录号 GTX634481

Rating
( Average 5 based on 1 users reviews)
实验应用
Immunocytochemistry/ Immunofluorescence(ICC/IF)
( Average 5 based on 1 users reviews)
Anti-Tyrosine Hydroxylase antibody [GT234] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX634481
Date : Anonymous submitted on 04-Jun-2021
Score :
Application Tested : ICC/IF
Sample Species : Ms
Sample : Mouse Brain, 250μm slice, coronal section
Fixation : 4% PFA in PBS, 4°C, 16Hr
Permeabilization /Antigen retrieval : 2% Triton X-100 in PBS, 25°C, 48Hr
Primary Antibody : 1:100, 4°C, 48Hr
Figure Note : This antibody was used to detect Tyrosine Hydroxylase expression in mice brain tissue (~250μm thickness) by Immunofluorescence (IF). The figure shown dopamine neurons of the substantia nigra project to the striatum.
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SDS
PBS.pdf
产品包装 报价 (¥)
¥ 4000
¥ 1700
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