Tyrosine Hydroxylase抗体 [N1C1]

Name: Tyrosine Hydroxylase抗体 [N1C1]
Brand: GeneTex
SKU: GTX102424
Price: 4000.00 RMB
Rating: 4.5 (2 reviews)

Anti-Tyrosine Hydroxylase antibody [N1C1] used in IHC (Frozen sections) (IHC-Fr). GTX102424

Anti-Tyrosine Hydroxylase antibody [N1C1] used in IHC (Paraffin sections) (IHC-P). GTX102424

Anti-Tyrosine Hydroxylase antibody [N1C1] used in Western Blot (WB). GTX102424

Anti-Tyrosine Hydroxylase antibody [N1C1] used in IHC (Whole mount) (IHC-Wm). GTX102424

Anti-Tyrosine Hydroxylase antibody [N1C1] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX102424

目录号 GTX102424

文献引用 2

宿主	Rabbit
克隆	Polyclonal
同种型	IgG
实验应用	WB, ICC/IF, IHC-P, IHC-Fr, IHC-Wm
种属反应	Human, Mouse, Rat, Zebrafish
产品包装	100 μl (¥4000), 25 μl (¥1700)

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View product citations for antibody GTX102424 on CiteAb

产品应用

应用说明

*最佳稀释倍数与浓度应由研究人员确认

实验应用	稀释倍数
WB	1:500-1:3000
ICC/IF	1:100-1:1000
IHC-P	1:100-1:1000
IHC-Fr	1:100-1:1000
IHC-Wm	1:100-1:500

WB: 免疫印迹
ICC/IF: 细胞染色
IHC-P: 石蜡切片
IHC-Fr: 冰冻切片

预期分子量

59 kDa. ( Note )

阳性对照

IMR32

产品属性

形式

Liquid

存储溶液

PBS, 20% Glycerol

存放说明

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

浓度

0.57 mg/ml (Please refer to the vial label for the specific concentration.)

免疫原种属

Human

免疫原

Recombinant protein encompassing a sequence within the center region of human Tyrosine Hydroxylase. The exact sequence is proprietary.

纯化方式

Purified by antigen-affinity chromatography.

偶联

Unconjugated

RRID

AB_2038197

注意事项

仅供实验室使用。不适用于人类或动物的任何临床，治疗或诊断用途。不适合动物或人类食用。

别名

tyrosine hydroxylase , DYT14 , DYT5b , TYH

功能与背景

The protein encoded by this gene is involved in the conversion of tyrosine to dopamine. It is the rate-limiting enzyme in the synthesis of catecholamines, hence plays a key role in the physiology of adrenergic neurons. Mutations in this gene have been associated with autosomal recessive Segawa syndrome. Alternatively spliced transcript variants encoding different isoforms have been noted for this gene. [provided by RefSeq]

数据库

Gene ID: 7054 TH
UniProt: P07101 TH

研究领域

產品图片

GTX102424 IHC-Fr Image

Tyrosine Hydroxylase antibody [N1C1] detects Tyrosine Hydroxylase protein expression by immunohistochemical analysis.
Sample: Frozen sectioned E13.5 Rat brain.
Green: Tyrosine Hydroxylase protein stained by Tyrosine Hydroxylase antibody [N1C1] (GTX102424) diluted at 1:250.
Red: SOX2, stained by SOX2 antibody [GT1876] (GTX627404) diluted at 1:250.
Blue: Fluoroshield with DAPI (GTX30920).

GTX102424 IHC-Fr Image

Tyrosine Hydroxylase antibody [N1C1] detects Tyrosine Hydroxylase protein by immunohistochemical analysis.
Sample: Frozen sectioned adult mouse retina.
Green: Tyrosine Hydroxylase protein stained by Tyrosine Hydroxylase antibody [N1C1] (GTX102424) diluted at 1:250.
Red: beta Tubulin 3/ TUJ1, stained by beta Tubulin 3/ TUJ1 antibody [GT11710] (GTX631836) diluted at 1:250.
Blue: Fluoroshield with DAPI (GTX30920).

GTX102424 IHC-P Image

Tyrosine Hydroxylase antibody [N1C1] detects Tyrosine Hydroxylase protein in midbrain dopaminergic neurons by immunohistochemical analysis.
Sample: Paraffin-embedded mouse brain.
Green: Tyrosine Hydroxylase stained by Tyrosine Hydroxylase antibody [N1C1] (GTX102424) diluted at 1:500.
Blue: Fluoroshield with DAPI (GTX30920).
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

GTX102424 WB Image

Whole cell extract (30 μg) was separated by 10% SDS-PAGE, and the membrane was blotted with Tyrosine Hydroxylase antibody [N1C1] (GTX102424) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

GTX102424 IHC-P Image

Tyrosine Hydroxylase antibody [N1C1] detects Tyrosine Hydroxylase protein at cytoplasm by immunohistochemical analysis.
Sample: Paraffin-embedded mouse brain.
Tyrosine Hydroxylase stained by Tyrosine Hydroxylase antibody [N1C1] (GTX102424) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

GTX102424 IHC-Wm Image

Tyrosine Hydroxylase antibody [N1C1] detects Tyrosine Hydroxylase protein on zebrafish by whole mount immunohistochemical analysis.
Sample: 2 days-post-fertilization zebrafish embryo.
Tyrosine Hydroxylase antibody [N1C1] (GTX102424) dilution: 1:100.

GTX102424 IHC-P Image

Tyrosine Hydroxylase antibody [N1C1] detects Tyrosine Hydroxylase protein at cytoplasm by immunohistochemical analysis.
Sample: Paraffin-embedded mouse brain.
Tyrosine Hydroxylase stained by Tyrosine Hydroxylase antibody [N1C1] (GTX102424) diluted at 1:1000.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

GTX102424 IHC-P Image

Tyrosine Hydroxylase antibody [N1C1] detects Tyrosine Hydroxylase protein at cell membrane and cytoplasm by immunohistochemical analysis.
Sample: Paraffin-embedded mouse brain.
Tyrosine Hydroxylase stained by Tyrosine Hydroxylase antibody [N1C1] (GTX102424) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

GTX102424 ICC/IF Image

Tyrosine Hydroxylase antibody [N1C1] detects Tyrosine Hydroxylase protein at cytoplasm by immunofluorescent analysis.
Sample: SK-N-SH cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: Tyrosine Hydroxylase protein stained by Tyrosine Hydroxylase antibody [N1C1] (GTX102424) diluted at 1:200.
Red: alpha Tubulin, a cytoskeleton marker, stained by alpha Tubulin antibody [GT114] (GTX628802) diluted at 1:1000.
Blue: Hoechst 33342 staining.

GTX102424 IHC-P Image

GTX102424 WB Image

Various tissue extracts (50 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with Tyrosine Hydroxylase antibody [N1C1] (GTX102424) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

GTX102424 IHC-P Image

Tyrosine Hydroxylase antibody [N1C1] detects Tyrosine Hydroxylase protein at cytoplasm by immunohistochemical analysis.
Sample: Paraffin-embedded rat brain.
Tyrosine Hydroxylase stained by Tyrosine Hydroxylase antibody [N1C1] (GTX102424) diluted at 1:1000.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

GTX102424 ICC/IF Image

Tyrosine Hydroxylase antibody [N1C1] detects Tyrosine Hydroxylase protein at cytoplasm by immunofluorescent analysis.
Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: Tyrosine Hydroxylase protein stained by Tyrosine Hydroxylase antibody [N1C1] (GTX102424) diluted at 1:200.
Red: alpha Tubulin, a cytoskeleton marker, stained by alpha Tubulin antibody [GT114] (GTX628802) diluted at 1:1000.
Blue: Hoechst 33342 staining.

GTX102424 IHC-Wm Image

GTX102424 IHC-P Image

文献引用

Application Reference

S Chen et al. Environ Sci Technol 2023; 57 (48) : 19407-19418 Neurotoxicity of the Cu(OH)2 Nanopesticide through Perturbing Multiple Neurotransmitter Pathways in Developing Zebrafish

实验应用 : WB

Lin W et al. iScience 2023; 26 (4) : 106285 Dendritic spine formation and synapse maturation in transcription factor-induced human iPSC-derived neurons.

实验应用 : ICC/IF

种属反应 : Human

我要回报文献

产品评论

Tyrosine Hydroxylase antibody [N1C1] 目录号 GTX102424

Rating

( Average 4.5 based on 2 users reviews)

实验应用

Western Blot(WB)		( Average 5 based on 1 users reviews)
IHC (Paraffin sections)(IHC-P)		( Average 4 based on 1 users reviews)

Date : Anonymous submitted on 16-Sep-2021

Score :

Application Tested : WB

Sample Species : Hu

Sample : IMR32 Neuroblast (ATCC CCL-127)

Amount used : 35μg

Blocking : 5% BSA in PBST, 25°C, 1Hr

Primary Antibody : 0.57μg/ml, 4°C, 16Hr

条带说明 :
Lane 1 : IMR-32 (ATCC CCL-127) as control.
Lane 2-4 : IMR-32 (ATCC CCL-127) were differentiated and harvested on different days.

Date : Anonymous submitted on 28-Jun-2016

Score :

Application Tested : IHC-P

Sample Species : Ms

Sample : Retina tissue

Blocking : 20% normal donkey serum and 0.3% Triton X-100 in PBS, 25°C, 1Hr

Fixation : 4%Paraformaldehyde+0.01%Glutaraldehyde in PBS

Primary Antibody : 1:250, 4°C, 24Hr

我要评论产品

Application Reference

S Chen et al. Environ Sci Technol 2023; 57 (48):19407-19418 Neurotoxicity of the Cu(OH)2 Nanopesticide through Perturbing Multiple Neurotransmitter Pathways in Developing Zebrafish

实验应用 : WB

Lin W et al. iScience 2023; 26 (4):106285 Dendritic spine formation and synapse maturation in transcription factor-induced human iPSC-derived neurons.

实验应用 : ICC/IF

种属反应 : Human

SDS
PBS.pdf
Glycerol.pdf
Proclin.pdf

产品包装	报价 (¥)
100 μl	¥ 4000
25 μl	¥ 1700
Qty

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	http://www.neobioscience.com
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Tyrosine Hydroxylase抗体 [N1C1]

目录号 GTX102424

宿主

克隆

同种型

实验应用

种属反应

产品应用

应用说明

预期分子量

阳性对照

产品属性

形式

存储溶液

存放说明

浓度

免疫原种属

免疫原

纯化方式

偶联

RRID

注意事项

相关讯息

别名

功能与背景

数据库

研究领域

產品图片

GTX102424 IHC-Fr Image

GTX102424 IHC-Fr Image

GTX102424 IHC-P Image

GTX102424 WB Image

GTX102424 IHC-P Image

GTX102424 IHC-Wm Image

GTX102424 IHC-P Image

GTX102424 IHC-P Image

GTX102424 ICC/IF Image

GTX102424 IHC-P Image

GTX102424 WB Image

GTX102424 IHC-P Image

GTX102424 ICC/IF Image

GTX102424 IHC-Wm Image

GTX102424 IHC-P Image

文献引用

Application Reference

产品评论

Tyrosine Hydroxylase antibody [N1C1] 目录号 GTX102424

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