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UBE3A抗体 [HL1660]

Recombinant antibody KO/KD Comparison Orthogonal
Anti-UBE3A antibody [HL1660] used in Western Blot (WB). GTX637258
Anti-UBE3A antibody [HL1660] used in Western Blot (WB). GTX637258
Anti-UBE3A antibody [HL1660] used in Western Blot (WB). GTX637258
Anti-UBE3A antibody [HL1660] used in Western Blot (WB). GTX637258

目录号 GTX637258

宿主

 Rabbit

克隆

 Monoclonal

克隆编号

 HL1660

同种型

 IgG

实验应用

 WB

种属反应

 Human
产品包装
100 μl (¥4000),
25 μl (¥1700)
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产品应用

应用说明

*最佳稀释倍数与浓度应由研究人员确认
实验应用 稀释倍数
WB 1:500-1:3000
WB: 免疫印迹
ICC/IF: 细胞染色
IHC-P: 石蜡切片
IHC-Fr: 冰冻切片

预期分子量

101 kDa. ( Note )

产品属性

形式

Liquid

存储溶液

PBS

存放说明

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

浓度

1 mg/ml (Please refer to the vial label for the specific concentration.)

免疫原种属

Human

免疫原

Recombinant protein encompassing a sequence within the center region of human UBE3A. The exact sequence is proprietary.

纯化方式

Affinity purified by Protein A.

偶联

Unconjugated

注意事项

仅供实验室使用。不适用于人类或动物的任何临床，治疗或诊断用途。不适合动物或人类食用。

相关讯息

别名

ubiquitin protein ligase E3A , ANCR , AS , E6-AP , EPVE6AP , HPVE6A

细胞定位

Cytoplasm,Nucleus

功能与背景

This gene encodes an E3 ubiquitin-protein ligase, part of the ubiquitin protein degradation system. This imprinted gene is maternally expressed in brain and biallelically expressed in other tissues. Maternally inherited deletion of this gene causes Angelman Syndrome, characterized by severe motor and intellectual retardation, ataxia, hypotonia, epilepsy, absence of speech, and characteristic facies. The protein also interacts with the E6 protein of human papillomavirus types 16 and 18, resulting in ubiquitination and proteolysis of tumor protein p53. Alternative splicing of this gene results in three transcript variants encoding three isoforms with different N-termini. Additional transcript variants have been described, but their full length nature has not been determined. [provided by RefSeq, Jul 2008]

数据库

研究领域

產品图片

Anti-UBE3A antibody [HL1660] used in Western Blot (WB). GTX637258

GTX637258 WB Image

KO/KD

Non-transfected (–) and transfected (+) HeLa whole cell extracts (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with UBE3A antibody [HL1660] (GTX637258) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.

Anti-UBE3A antibody [HL1660] used in Western Blot (WB). GTX637258

GTX637258 WB Image

Comparison

Various whole cell extracts (30 μg) were separated by 5% SDS-PAGE, and the membranes were blotted with UBE3A antibody [HL1660] (GTX637258) diluted at 1:1000 and competitor's antibody (Competitor) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

*The competitor is not affiliated with GeneTex and does not endorse this product.

Anti-UBE3A antibody [HL1660] used in Western Blot (WB). GTX637258

GTX637258 WB Image

Orthogonal

Various whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with UBE3A antibody [HL1660] (GTX637258) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced. Corresponding RNA expression data for the same cell lines are based on Human Protein Atlas program.

Anti-UBE3A antibody [HL1660] used in Western Blot (WB). GTX637258

GTX637258 WB Image

Various whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with UBE3A antibody [HL1660] (GTX637258) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

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产品包装 报价 (¥)
¥ 4000
¥ 1700
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