产品应用
应用说明
*最佳稀释倍数与浓度应由研究人员确认
| 实验应用 |
稀释倍数 |
| 1:100-1:1000 |
| 1:100-1:1000 |
WB: 免疫印迹
ICC/IF: 细胞染色
IHC-P: 石蜡切片
IHC-Fr: 冰冻切片
预期分子量
预测种属反应
Bovine, Pig, Xenopus tropicalis, Rhesus Monkey(>80% identity)
产品属性
形式
Liquid
存储溶液
PBS, 20% Glycerol
存放说明
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
浓度
1.26 mg/ml (Please refer to the vial label for the specific concentration.)
免疫原种属
Human
免疫原
Recombinant protein encompassing a sequence within the center region of human UBE3A. The exact sequence is proprietary.
纯化方式
Purified by antigen-affinity chromatography.
偶联
Unconjugated
RRID
AB_2885224
注意事项
仅供实验室使用。不适用于人类或动物的任何临床，治疗或诊断用途。不适合动物或人类食用。
相关讯息
别名
ubiquitin protein ligase E3A , ANCR , AS , E6-AP , EPVE6AP , HPVE6A
细胞定位
Nucleus
功能与背景
This gene encodes an E3 ubiquitin-protein ligase, part of the ubiquitin protein degradation system. This imprinted gene is maternally expressed in brain and biallelically expressed in other tissues. Maternally inherited deletion of this gene causes Angelman Syndrome, characterized by severe motor and intellectual retardation, ataxia, hypotonia, epilepsy, absence of speech, and characteristic facies. The protein also interacts with the E6 protein of human papillomavirus types 16 and 18, resulting in ubiquitination and proteolysis of tumor protein p53. Alternative splicing of this gene results in three transcript variants encoding three isoforms with different N-termini. Additional transcript variants have been described, but their full length nature has not been determined. [provided by RefSeq]
数据库
研究领域
產品图片
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GTX101092 IHC-P Image
UBE3A antibody detects UBE3A protein at cytoplasm and nucleus by immunohistochemical analysis.Sample: Paraffin-embedded mouse brain.UBE3A stained by UBE3A antibody (GTX101092) diluted at 1:500.Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
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GTX101092 IHC-P Image
UBE3A antibody detects UBE3A protein at nucleus on human ovarian carcinoma by immunohistochemical analysis.
Sample: Paraffin-embedded human ovarian carcinoma.
UBE3A antibody (GTX101092) dilution: 1:500.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min
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GTX101092 ICC/IF Image
UBE3A antibody detects UBE3A protein at nucleus by immunofluorescent analysis.Sample: DIV10 rat E18 primary cortical neuron cells cells were fixed in 4% paraformaldehyde at RT for 15 min.Green: UBE3A stained by UBE3A antibody (GTX101092) diluted at 1:500.Red: Tau, a Tau marker, stained by Tau antibody [GT287] (GTX634809) diluted at 1:500.Blue: Fluoroshield with DAPI (GTX30920).
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GTX101092 IHC-P Image
UBE3A antibody detects UBE3A protein at cytoplasm and nucleus by immunohistochemical analysis.Sample: Paraffin-embedded mouse brain.UBE3A stained by UBE3A antibody (GTX101092) diluted at 1:500.Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
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GTX101092 IHC-P Image
UBE3A antibody detects UBE3A protein at cytosol on mouse middle brain by immunohistochemical analysis.
Sample: Paraffin-embedded mouse middle brain.
UBE3A antibody (GTX101092) dilution: 1:500.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min
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