产品应用
应用说明
*最佳稀释倍数与浓度应由研究人员确认
实验应用 |
稀释倍数 |
1:500-1:3000 |
1:100-1:1000 |
1:100-1:1000 |
WB: 免疫印迹
ICC/IF: 细胞染色
IHC-P: 石蜡切片
IHC-Fr: 冰冻切片
预期分子量
阳性对照
HeLaS3
预测种属反应
Rat, Bovine, Chicken, Pig, Xenopus laevis, Rhesus Monkey(>80% identity)
产品属性
形式
Liquid
存储溶液
0.1M Tris, 0.1M Glycine, 10% Glycerol
存放说明
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
浓度
0.7 mg/ml (Please refer to the vial label for the specific concentration.)
免疫原种属
Human
免疫原
Recombinant protein encompassing a sequence within the center region of human UGP2. The exact sequence is proprietary.
纯化方式
Purified by antigen-affinity chromatography.
偶联
Unconjugated
RRID
AB_2038207
注意事项
仅供实验室使用。不适用于人类或动物的任何临床,治疗或诊断用途。不适合动物或人类食用。
相关讯息
别名
UDP-glucose pyrophosphorylase 2 , UDPG , UDPGP , UDPGP2 , UGP1 , UGPP1 , UGPP2 , pHC379
细胞定位
Cytoplasm
功能与背景
The enzyme encoded by this gene is an important intermediary in mammalian carbohydrate interconversions. It transfers a glucose moiety from glucose-1-phosphate to MgUTP and forms UDP-glucose and MgPPi. In liver and muscle tissue, UDP-glucose is a direct precursor of glycogen; in lactating mammary gland it is converted to UDP-galactose which is then converted to lactose. The eukaryotic enzyme has no significant sequence similarity to the prokaryotic enzyme. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq]
数据库
研究领域
產品图片
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GTX107967 IHC-P Image
Immunohistochemical analysis of paraffin-embedded DLD-1 xenograft , using UGP2(GTX107967) antibody at 1:500 dilution.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
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GTX107967 IHC-P Image
UGP2 antibody [N3C3-2] detects UGP2 protein at cytoplasm in mouse liver by immunohistochemical analysis. Sample: Paraffin-embedded mouse liver. UGP2 antibody [N3C3-2] (GTX107967) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
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GTX107967 ICC/IF Image
Immunofluorescence analysis of paraformaldehyde-fixed HeLa, using UGP2(GTX107967) antibody at 1:200 dilution.
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GTX107967 WB Image
Sample (30 ug of whole cell lysate) A: Hela S3 7.5% SDS PAGE GTX107967 diluted at 1:1000
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GTX107967 IHC-P Image
UGP2 antibody [N3C3-2] detects UGP2 protein at cytoplasm in mouse duodenum by immunohistochemical analysis. Sample: Paraffin-embedded mouse duodenum. UGP2 antibody [N3C3-2] (GTX107967) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
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GTX107967 WB Image
The data was published in the journal Elife in 2016.PMID: 26981769
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文献引用
产品评论
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