产品应用
应用说明
*最佳稀释倍数与浓度应由研究人员确认
实验应用 |
稀释倍数 |
1:1000-1:10000 |
Assay dependent |
WB: 免疫印迹
ICC/IF: 细胞染色
IHC-P: 石蜡切片
IHC-Fr: 冰冻切片
预期分子量
产品属性
形式
Liquid
存储溶液
PBS
存放说明
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
浓度
1 mg/ml (Please refer to the vial label for the specific concentration.)
免疫原种属
Human
免疫原
Recombinant fragment of human UHRF1.
纯化方式
Affinity purified by Protein A.
偶联
Unconjugated
注意事项
仅供实验室使用。不适用于人类或动物的任何临床,治疗或诊断用途。不适合动物或人类食用。
相关讯息
别名
ubiquitin like with PHD and ring finger domains 1 , ICBP90 , Np95 , RNF106 , TDRD22 , hNP95 , hUHRF1 , huNp95
细胞定位
Nucleus
功能与背景
This gene encodes a member of a subfamily of RING-finger type E3 ubiquitin ligases. The protein binds to specific DNA sequences, and recruits a histone deacetylase to regulate gene expression. Its expression peaks at late G1 phase and continues during G2 and M phases of the cell cycle. It plays a major role in the G1/S transition by regulating topoisomerase IIalpha and retinoblastoma gene expression, and functions in the p53-dependent DNA damage checkpoint. It is regarded as a hub protein for the integration of epigenetic information. This gene is up-regulated in various cancers, and it is therefore considered to be a therapeutic target. Multiple transcript variants encoding different isoforms have been found for this gene. A related pseudogene exists on chromosome 12. [provided by RefSeq, Feb 2014]
数据库
研究领域
產品图片
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GTX638342 WB Image
Non-transfected (–) and transfected (+) HeLa whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with UHRF1 antibody [HL2287] (GTX638342) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
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GTX638342 WB Image
Various whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with UHRF1 antibody [HL2287] (GTX638342) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody. Corresponding RNA expression data for the same cell lines are based on Human Protein Atlas program.
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GTX638342 WB Image
Mouse tissue extract (50 μg) was separated by 7.5% SDS-PAGE, and the membrane was blotted with UHRF1 antibody [HL2287] (GTX638342) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.
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GTX638342 IHC-P Image
UHRF1 antibody [HL2287] detects UHRF1 protein by immunohistochemical analysis.Sample: Paraffin-embedded rat tissues.UHRF1 stained by UHRF1 antibody [HL2287] (GTX638342) diluted at 1:100.Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
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GTX638342 IHC-P Image
UHRF1 antibody [HL2287] detects UHRF1 protein by immunohistochemical analysis.Sample: Paraffin-embedded mouse tissues.UHRF1 stained by UHRF1 antibody [HL2287] (GTX638342) diluted at 1:100.Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
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