产品说明
产品应用
应用说明
*最佳稀释倍数与浓度应由研究人员确认
实验应用 |
稀释倍数 |
1:100-1:200 |
Note :
IHC-P
Manual staining : Heat-induced antigen retrieval for 5 minutes in an autoclave at 121ºC in pH 7.8 Tris-EDTA-based Target Retrieval Solution buffer.
This antibody has been validated by Autostainer including Agilent/Dako - Autostainer Link 48, Bond RX-Lecia, and Roche-Ventana Discovery ULTRA. The detailed steps by Autostainer please refer to the protocol.
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WB: 免疫印迹
ICC/IF: 细胞染色
IHC-P: 石蜡切片
IHC-Fr: 冰冻切片
预期分子量
阳性对照
Urinary bladder: A strong membranous and cytoplasmic UPK1b immunostaining should be seen in the urothelium (the staining can be limited to the top cell layers or equally involve all cell layers).
Negative Control
Colon: UPK1b immunostaining should be absent in all cells of the colon mucosa.
产品属性
形式
Liquid
存储溶液
PBS, 0.05% BSA (Please contact us for PBS only format)
存放说明
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
浓度
0.2 mg/ml (Please refer to the vial label for the specific concentration.)
免疫原种属
Human
免疫原
Recombinant fragment (around aa 109-229) of human Uroplakin 1B (UPK1B) protein
纯化方式
Protein A/G purified
偶联
Unconjugated
注意事项
仅供实验室使用。不适用于人类或动物的任何临床,治疗或诊断用途。不适合动物或人类食用。
相关讯息
别名
uroplakin 1B , TSPAN20 , UPIB , UPK1
细胞定位
Membrane; Multi-pass membrane protein
功能与背景
The protein encoded by this gene is a member of the transmembrane 4 superfamily, also known as the tetraspanin family. Most of these members are cell-surface proteins that are characterized by the presence of four hydrophobic domains. The proteins mediate signal transduction events that play a role in the regulation of cell development, activation, growth and motility. This encoded protein is found in the asymmetrical unit membrane (AUM) where it can form a complex with other transmembrane 4 superfamily proteins. It may play a role in normal bladder epithelial physiology, possibly in regulating membrane permeability of superficial umbrella cells or in stabilizing the apical membrane through AUM/cytoskeletal interactions. The use of alternate polyadenylation sites has been found for this gene. [provided by RefSeq, Jul 2008]
数据库
研究领域
產品图片
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GTX04450 IHC-P Image
IHC-P analysis of human urinary bladder urothelium tissue section using GTX04450 UPK1B antibody [MSVA-734M] HistoMAX™. A strong UPK1B immunostaining of all urothelial cells is seen in this urinary bladder sample.
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GTX04450 IHC-P Image
IHC-P analysis of human urinary bladder muscle invasive urothelial carcinoma tissue section using GTX04450 UPK1B antibody [MSVA-734M] HistoMAX™. Muscle invasive urothelial carcinoma of the urinary bladder showing a strong UPK1B immunostaining of tumor cells.
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GTX04450 IHC-P Image
IHC-P analysis of human colon mucosa tissue section using GTX04450 UPK1B antibody [MSVA-734M] HistoMAX™. UPK1B immunostaining is absent in all cells of the colon mucosa.
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