产品应用
应用说明
*最佳稀释倍数与浓度应由研究人员确认
实验应用 |
稀释倍数 |
1:500-1:3000 |
1:100-1:1000 |
WB: 免疫印迹
ICC/IF: 细胞染色
IHC-P: 石蜡切片
IHC-Fr: 冰冻切片
预期分子量
阳性对照
mouse brain , rat brain , 293T , A431 , HeLa , HepG2
预测种属反应
Bovine, Chimpanzee, Rhesus Monkey(>80% identity)
产品属性
形式
Liquid
存储溶液
PBS
存放说明
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
浓度
1 mg/ml (Please refer to the vial label for the specific concentration.)
免疫原种属
Human
免疫原
Recombinant protein encompassing a sequence within the center region of human UQCRC1. The exact sequence is proprietary.
纯化方式
Affinity purified by Protein G.
偶联
Unconjugated
RRID
AB_2888211
注意事项
仅供实验室使用。不适用于人类或动物的任何临床,治疗或诊断用途。不适合动物或人类食用。
相关讯息
别名
ubiquinol-cytochrome c reductase core protein 1 , D3S3191 , QCR1 , UQCR1
细胞定位
Mitochondrion inner membrane
功能与背景
This is a component of the ubiquinol-cytochrome c reductase complex (complex III or cytochrome b-c1 complex), which is part of the mitochondrial respiratory chain. This protein may mediate formation of the complex between cytochromes c and c1.
数据库
研究领域
產品图片
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GTX630413 WB Image
UQCRC1 antibody [GT139] detects UQCRC1 protein by western blot analysis. A. 50 μg mouse brain lysate/extract 7.5 % SDS-PAGE UQCRC1 antibody [GT139] (GTX630413) dilution: 1:1000
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GTX630413 WB Image
UQCRC1 antibody [GT139] detects UQCRC1 protein by western blot analysis. A. 50 μg rat brain lysate/extract 10 % SDS-PAGE UQCRC1 antibody [GT139] (GTX630413) dilution: 1:1000
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GTX630413 ICC/IF Image
UQCRC1 antibody [GT139] detects UQCRC1 protein at mitochondria by immunofluorescent analysis. Sample: HeLa cells were fixed in 4% paraformaldehyde at room temperature. Green: UQCRC1 protein stained by UQCRC1 antibody [GT139] (GTX630413) diluted at 1:100. Blue: Hoechst 33342 staining. Scale bar = 10 μm.
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GTX630413 WB Image
UQCRC1 antibody [GT139] detects UQCRC1 protein by western blot analysis. A. 30 μg 293T whole cell lysate/extract B. 30 μg A431 whole cell lysate/extract C. 30 μg HeLa whole cell lysate/extract D. 30 μg HepG2 whole cell lysate/extract 10 % SDS-PAGE UQCRC1 antibody [GT139] (GTX630413) dilution: 1:1000
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GTX630413 WB Image
Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with UQCRC1 antibody [GT139] (GTX630413) diluted at 1:500.
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