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UQCRC2抗体

Comparison Orthogonal
Anti-UQCRC2 antibody used in Western Blot (WB). GTX114873
Anti-UQCRC2 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX114873
Anti-UQCRC2 antibody used in Western Blot (WB). GTX114873
Anti-UQCRC2 antibody used in Immunoprecipitation (IP). GTX114873
Anti-UQCRC2 antibody used in IHC (Paraffin sections) (IHC-P). GTX114873

目录号 GTX114873

文献引用 8

宿主

 Rabbit

克隆

 Polyclonal

同种型

 IgG

实验应用

 WB, ICC/IF, IHC-P, IP, PLA

种属反应

 Human, Mouse, Rat
产品包装
100 μl (¥4000),
25 μl (¥1700)
查看所有 UQCRC2 的产品

产品应用

应用说明

*最佳稀释倍数与浓度应由研究人员确认
实验应用 稀释倍数
WB 1:5000-1:20000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
IP 1:100-1:500
PLA Assay dependent
WB: 免疫印迹
ICC/IF: 细胞染色
IHC-P: 石蜡切片
IHC-Fr: 冰冻切片

预期分子量

48 kDa. ( Note )

阳性对照

mouse brain , rat brain , Jurkat , Raji , NCI-H929

预测种属反应

Bovine, Chimpanzee, Rhesus Monkey(>80% identity)

产品属性

形式

Liquid

存储溶液

PBS, 1% BSA, 20% Glycerol

存放说明

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

浓度

1 mg/ml (Please refer to the vial label for the specific concentration.)

免疫原种属

Human

免疫原

Recombinant protein encompassing a sequence within the center region of human UQCRC2. The exact sequence is proprietary.

纯化方式

Purified by antigen-affinity chromatography.

偶联

Unconjugated

RRID

AB_11164243

注意事项

仅供实验室使用。不适用于人类或动物的任何临床，治疗或诊断用途。不适合动物或人类食用。

相关讯息

别名

ubiquinol-cytochrome c reductase core protein 2 , MC3DN5 , QCR2 , UQCR2

细胞定位

Mitochondrion inner membrane; Peripheral membrane protein; Matrix side

功能与背景

This is a component of the ubiquinol-cytochrome c reductase complex (complex III or cytochrome b-c1 complex), which is part of the mitochondrial respiratory chain. The core protein 2 is required for the assembly of the complex.

数据库

研究领域

產品图片

Anti-UQCRC2 antibody used in Western Blot (WB). GTX114873

GTX114873 WB Image

Comparison

Various whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membranes were blotted with UQCRC2 antibody (GTX114873) diluted at 1:10000 and competitor's antibody (14742-1-AP) diluted at 1:10000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

*The competitor is not affiliated with GeneTex and does not endorse this product.

Anti-UQCRC2 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX114873

GTX114873 ICC/IF Image

Immunofluorescence analysis of paraformaldehyde-fixed A431, using UQCRC2(GTX114873) antibody at 1:500 dilution.

Anti-UQCRC2 antibody used in Western Blot (WB). GTX114873

GTX114873 WB Image

Various tissue extracts (50 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with UQCRC2 antibody (GTX114873) diluted at 1:10000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-UQCRC2 antibody used in Immunoprecipitation (IP). GTX114873

GTX114873 IP Image

UQCRC2 antibody immunoprecipitates UQCRC2 protein in IP experiments.
IP samples: HepG2 whole cell extract
A. 35 μg HepG2 whole cell extract
B. Control with 4 μg of preimmune Rabbit IgG
C. Immunoprecipitation of UQCRC2 protein by 4 μg UQCRC2 antibody (GTX114873)
10 % SDS-PAGE
The immunoprecipitated UQCRC2 protein was detected by UQCRC2 antibody (GTX114873) diluted at 1:5000.
[EasyBlot anti-rabbit IgG (GTX221666-01) was used as a secondary reagent]
Anti-UQCRC2 antibody used in IHC (Paraffin sections) (IHC-P). GTX114873

GTX114873 IHC-P Image

UQCRC2 antibody detects UQCRC2 protein at cytoplasm on human breast cancer by immunohistochemical analysis.
Sample: Paraffin-embedded breast cancer.
UQCRC2 antibody (GTX114873) dilution: 1:250.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

文献引用

Application Reference

Lee H et al. Nat Commun 2023; 14 (1) : 6721 The mitochondrial fusion protein OPA1 is dispensable in the liver and its absence induces mitohormesis to protect liver from drug-induced injury.
Avolio R et al. Genome Res 2023; 33 (8) : 1242-1257 Cytosolic and mitochondrial translation elongation are coordinated through the molecular chaperone TRAP1 for the synthesis and import of mitochondrial proteins.
Matassa DS et al. Cancer Cell Int 2022; 22 (1) : 402 Regulation of mitochondrial complex III activity and assembly by TRAP1 in cancer cells.
实验应用 : WB, PLA
种属反应 : Human
Mingming Zhao et al. Nat Commun 2022; 13 (1) : 1757 Gut microbiota production of trimethyl-5-aminovaleric acid reduces fatty acid oxidation and accelerates cardiac hypertrophy.
实验应用 : WB
种属反应 : Mouse
Lee H et al. J Biol Chem 2020; 295 (19) : 6543-6560 The short variant of optic atrophy 1 (OPA1) improves cell survival under oxidative stress.
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Application Reference
Lee H et al. Nat Commun 2023; 14 (1):6721 The mitochondrial fusion protein OPA1 is dispensable in the liver and its absence induces mitohormesis to protect liver from drug-induced injury.
Avolio R et al. Genome Res 2023; 33 (8):1242-1257 Cytosolic and mitochondrial translation elongation are coordinated through the molecular chaperone TRAP1 for the synthesis and import of mitochondrial proteins.
Matassa DS et al. Cancer Cell Int 2022; 22 (1):402 Regulation of mitochondrial complex III activity and assembly by TRAP1 in cancer cells.
实验应用 : WB, PLA
种属反应 : Human
Mingming Zhao et al. Nat Commun 2022; 13 (1):1757 Gut microbiota production of trimethyl-5-aminovaleric acid reduces fatty acid oxidation and accelerates cardiac hypertrophy.
实验应用 : WB
种属反应 : Mouse
Lee H et al. J Biol Chem 2020; 295 (19):6543-6560 The short variant of optic atrophy 1 (OPA1) improves cell survival under oxidative stress.
Imami K et al. Mol Cell 2018; 72 (1):84-98.e9 Phosphorylation of the Ribosomal Protein RPL12/uL11 Affects Translation during Mitosis.
实验应用 : WB
种属反应 : Human
Takahashi K et al. Exp Gerontol 2017; 98:217-223 Optic atrophy 1 mediates coenzyme Q-responsive regulation of respiratory complex IV activity in brain mitochondria.
实验应用 : IP
种属反应 : Mouse
Lee H et al. J Biol Chem 2017; (Epub) The Short Variant of the Mitochondrial Dynamin OPA1 Maintains Mitochondrial Energetics and Cristae Structure.
实验应用 : WB
种属反应 : Human
SDS
PBS.pdf
Glycerol.pdf
Thimerosal.pdf
产品包装 报价 (¥)
¥ 4000
¥ 1700
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