VCP抗体 [N1N2], N-term

Name: VCP抗体 [N1N2], N-term
Brand: GeneTex
SKU: GTX113030
Price: 4000.00 RMB
Rating: 5 (1 reviews)

Anti-VCP antibody [N1N2], N-term used in IHC (Paraffin sections) (IHC-P). GTX113030

Anti-VCP antibody [N1N2], N-term used in Western Blot (WB). GTX113030

Anti-VCP antibody [N1N2], N-term used in IHC (Whole mount) (IHC-Wm). GTX113030

目录号 GTX113030

文献引用 27

宿主	Rabbit
克隆	Polyclonal
同种型	IgG
实验应用	WB, IHC-P, IHC-Wm
种属反应	Human, Mouse, Rat, Zebrafish, Dog
产品包装	100 μl (¥4000), 25 μl (¥1700)

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View product citations for antibody GTX113030 on CiteAb

产品应用

应用说明

*最佳稀释倍数与浓度应由研究人员确认

实验应用	稀释倍数
WB	1:1000-1:10000
IHC-P	1:100-1:1000
IHC-Wm	Assay dependent

WB: 免疫印迹
ICC/IF: 细胞染色
IHC-P: 石蜡切片
IHC-Fr: 冰冻切片

预期分子量

89 kDa. ( Note )

阳性对照

Mouse brain , rat brain , A549 , H1299 , HCT116

预测种属反应

Bovine, Chicken, Pig, Xenopus tropicalis, Chimpanzee(>80% identity)

产品属性

形式

Liquid

存储溶液

PBS, 1% BSA, 20% Glycerol

存放说明

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

浓度

0.3 mg/ml (Please refer to the vial label for the specific concentration.)

免疫原种属

Human

免疫原

Recombinant protein encompassing a sequence within the N-terminus region of human VCP. The exact sequence is proprietary.

纯化方式

Purified by antigen-affinity chromatography.

偶联

Unconjugated

RRID

AB_1952542

注意事项

仅供实验室使用。不适用于人类或动物的任何临床，治疗或诊断用途。不适合动物或人类食用。

别名

valosin containing protein , CDC48 , TERA , p97

细胞定位

Cytoplasm , cytosol , Nucleus

功能与背景

The protein encoded by this gene is a member of a family that includes putative ATP-binding proteins involved in vesicle transport and fusion, 26S proteasome function, and assembly of peroxisomes. This protein, as a structural protein, is associated with clathrin, and heat-shock protein Hsc70, to form a complex. It has been implicated in a number of cellular events that are regulated during mitosis, including homotypic membrane fusion, spindle pole body function, and ubiquitin-dependent protein degradation. [provided by RefSeq]

数据库

Gene ID: 7415 VCP
UniProt: P55072 VCP

研究领域

產品图片

GTX113030 IHC-P Image

Immunohistochemical analysis of paraffin-embedded zebrafish tissue, using VCP antibody [N1N2], N-term (GTX113030) at 1:300 dilution.

GTX113030 WB Image

Various tissue extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with VCP antibody [N1N2], N-term (GTX113030) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

GTX113030 IHC-Wm Image

VCP antibody [N1N2], N-term detects Vcp protein on zebrafish by whole mount immunohistochemical analysis.
Sample: 1 day-post-fertilization zebrafish embryo.
VCP antibody [N1N2], N-term (GTX113030) dilution: 1:100.

GTX113030 WB Image

VCP antibody detects VCP protein by Western blot analysis.
A. 50 μg rat brain lysate/extract
7.5 % SDS-PAGE
VCP antibody (GTX113030) dilution: 1:1000

GTX113030 IHC-P Image

VCP antibody [N1N2], N-term detects VCP protein at cytoplasm and nucleus in human lung adenocarcinoma by immunohistochemical analysis.
Sample: Paraffin-embedded human lung adenocarcinoma.
VCP antibody [N1N2], N-term (GTX113030) diluted at 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

GTX113030 WB Image

Various whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with VCP antibody [N1N2], N-term (GTX113030) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

GTX113030 IHC-P Image

VCP antibody [N1N2], N-term detects VCP protein at nucleus and cytosol on rat brain stem by immunohistochemical analysis.
Sample: Paraffin-embedded rat brain stem.
VCP antibody [N1N2], N-term (GTX113030) dilution: 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

GTX113030 IHC-P Image

GTX113030 WB Image

Sample (50 ug of whole cell lysate)
A: mouse brain
7.5% SDS PAGE
GTX113030 diluted at 1:5000

GTX113030 IHC-P Image

Immunohistochemical analysis of paraffin-embedded Ca922 xenograft, using VCP(p97)(GTX113030) antibody at 1:500 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

GTX113030 WB Image

The data was published in the journal PLoS One in 2015. PMID: 26678046

GTX113030 WB Image

The data was published in the journal FEBS Open Bio in 2018.PMID: 30186749

GTX113030 WB Image

The data was published in the journal FEBS Open Bio in 2018.PMID: 30186749

GTX113030 WB Image

The data was published in the journal FEBS Open Bio in 2018.PMID: 30186749

GTX113030 WB Image

The data was published in the journal FEBS Open Bio in 2018.PMID: 30186749

GTX113030 WB Image

The data was published in the journal Sci Rep in 2017. PMID: 28655918

GTX113030 WB Image

The data was published in the 2022 in BMC Cancer. PMID: 35277124

文献引用

Application Reference

Kitamura N et al. J Vet Med Sci 2023; 85 (12) : 1319-1323 Protein phosphatase 6 promotes transforming growth factor-β signaling in mouse embryonic fibroblasts.

Kohyanagi N et al. J Biol Chem 2023; 300 (1) : 105584 PP2A inhibitor SET promotes mTORC1 and Bmi1 signaling through Akt activation and maintains the colony-formation ability of cancer cells.

Yamamoto M et al. J Vet Med Sci 2023; 85 (9) : 977-984 Protein phosphatase 6 regulates trametinib sensitivity, a mitogen-activated protein kinase kinase (MEK) inhibitor, by regulating MEK1/2-ERK1/2 signaling in canine melanoma cells.

Yomogi Shiota et al. Biomed Pharmacother 2023; 165 : 115079 Derivation of a new model of lung adenocarcinoma using canine lung cancer organoids for translational research in pulmonary medicine

实验应用 : WB

Hancock JL et al. J Exp Clin Cancer Res 2023; 42 (1) : 90 COMMD3 loss drives invasive breast cancer growth by modulating copper homeostasis.

实验应用 : WB

种属反应 : Human

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产品评论

VCP antibody [N1N2], N-term 目录号 GTX113030

Rating

( Average 5 based on 1 users reviews)

实验应用

Western Blot(WB)

( Average 5 based on 1 users reviews)

Date : Anonymous submitted on 21-Jan-2020

Score :

Application Tested : WB

Sample Species : Hu

Sample : HeLa cells

Primary Antibody : 1:20000, 4°C, 16Hr

我要评论产品

Application Reference

Kitamura N et al. J Vet Med Sci 2023; 85 (12):1319-1323 Protein phosphatase 6 promotes transforming growth factor-β signaling in mouse embryonic fibroblasts.

Kohyanagi N et al. J Biol Chem 2023; 300 (1):105584 PP2A inhibitor SET promotes mTORC1 and Bmi1 signaling through Akt activation and maintains the colony-formation ability of cancer cells.

Yamamoto M et al. J Vet Med Sci 2023; 85 (9):977-984 Protein phosphatase 6 regulates trametinib sensitivity, a mitogen-activated protein kinase kinase (MEK) inhibitor, by regulating MEK1/2-ERK1/2 signaling in canine melanoma cells.

Yomogi Shiota et al. Biomed Pharmacother 2023; 165:115079 Derivation of a new model of lung adenocarcinoma using canine lung cancer organoids for translational research in pulmonary medicine

实验应用 : WB

Hancock JL et al. J Exp Clin Cancer Res 2023; 42 (1):90 COMMD3 loss drives invasive breast cancer growth by modulating copper homeostasis.

实验应用 : WB

种属反应 : Human

Guettsches AK et al. Biomedicines 2022; 10 (10) FYCO1 Increase and Effect of Arimoclomol-Treatment in Human VCP-Pathology.

种属反应 : Human

Ryouichi Tsunedomi et al. BMC Cancer 2022; 22 (1):260 Elevated expression of RAB3B plays important roles in chemoresistance and metastatic potential of hepatoma cells.

实验应用 : WB

种属反应 : Human

Kitamura N et al. J Biochem 2021; 170 (1):131-138 Protein phosphatase 6 promotes neurite outgrowth by promoting mTORC2 activity in N2a cells.

Fujiwara N et al. Biochem Biophys Res Commun 2021; 552:191-195 Protein phosphatase 6 dissociates the Beclin 1/Vps34 complex and inhibits autophagy.

Fujiwara Y et al. Pancreas 2021; 50 (3):405-413 Pancreatic Cancer Stem-Like Cells With High Calreticulin Expression Associated With Immune Surveillance.

实验应用 : WB

种属反应 : Human

Fujimoto T et al. Oncol Lett 2021; 21 (1):30 Cathepsin B is highly expressed in pancreatic cancer stem-like cells and is associated with patients' surgical outcomes.

实验应用 : WB

种属反应 : Human

Fujiwara N et al. Cancer Sci 2020; 111 (12):4371-4380 Autophagy regulates levels of tumor suppressor enzyme protein phosphatase 6.

实验应用 : WB

种属反应 : Human

Ikeda S et al. J Biochem 2020; Involvement of PP2A Methylation in the Adipogenic Differentiation of Bone Marrow Derived Mesenchymal Stem Cell.

实验应用 : WB

种属反应 : Mouse

Tsuji S et al. J Vet Med Sci 2019; Efficacy of an anti-cancer strategy targeting SET in canine osteosarcoma.

实验应用 : WB

种属反应 : Dog

Chernis N et al. Am J Physiol Cell Physiol 2019; Bisphenol AF promotes inflammation in human white adipocytes.

实验应用 : WB

种属反应 : Human

Yabe R et al. FEBS Open Bio 2018; 8 (9):1486-1496 A stable association with PME-1 may be dispensable for PP2A demethylation - implications for the detection of PP2A methylation and immunoprecipitation.

Ryotaro Yabe et al. FEBS openbio 2018; (Epub) A stable association with PME-1 may be dispensable for PP2A demethylation: Implications for the detection of PP2A methylation and immunoprecipitation.

实验应用 : WB

种属反应 : Mouse

Koh EH et al. Diabetes 2018; (Epub) mir-30a remodels subcutaneous adipose tissue inflammation to improve * sensitivity in obesity.

实验应用 : WB

种属反应 : Mouse

Kake S et al. Sci Rep 2017; 7 (1):4279 The role of SET/I2PP2A in canine mammary tumors.

实验应用 : WB

种属反应 : Dog

Kake S et al. Oncol Rep 2017; 37 (2):1100-1106 Death-associated protein kinase 3 controls the tumor progression of A549 cells through ERK MAPK/c-Myc signaling.

种属反应 : Human

Fujiwara N et al. J Biol Chem 2016; 291 (20):10858-66 Regulation of Beclin 1 Protein Phosphorylation and Autophagy by Protein Phosphatase 2A (PP2A) and Death-associated Protein Kinase 3 (DAPK3).

实验应用 : WB

种属反应 : Mouse

Tsuji S et al. J Vet Med Sci 2016; (Epub) Anti-tumor effects of * on canine lymphoma.

实验应用 : WB

种属反应 : Dog

Enjoji S et al. J Vet Med Sci 2015; 77 (11):1451-6 The therapeutic effects of SET/I2PP2A inhibitors on canine melanoma.

实验应用 : WB

种属反应 : Dog

Yabe R et al. PLoS One 2015; 10 (12):e0145226 Protein Phosphatase Methyl-Esterase PME-1 Protects Protein Phosphatase 2A from Ubiquitin/Proteasome Degradation.

实验应用 : WB

种属反应 : Mouse

May EW et al. Pharmacol Res 2014; 90C:43117 identification of up- and down-regulated proteins in *-resistant uterine cancer cells: reticulocalbin-1 plays a key role in the development of *-associated resistance.

实验应用 : WB

种属反应 : Human

Huang CH et al. J Biol Chem 2014; 289 (7):4444-54 Role of HERP and a HERP-related protein in HRD1-dependent protein degradation at the endoplasmic reticulum.

实验应用 : WB

种属反应 : Human

Huang CH et al. J Biol Chem 2013; 288 (35):25330-9 Derlin2 protein facilitates HRD1-mediated retro-translocation of sonic hedgehog at the endoplasmic reticulum.

实验应用 : WB

种属反应 : Human

SDS
PBS.pdf
Glycerol.pdf
Proclin.pdf

产品包装	报价 (¥)
100 μl	¥ 4000
25 μl	¥ 1700
Qty

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	http://www.neobioscience.com
	genetex@neobioscience.com

VCP抗体 [N1N2], N-term

目录号 GTX113030

宿主

克隆

同种型

实验应用

种属反应

产品应用

应用说明

预期分子量

阳性对照

预测种属反应

产品属性

形式

存储溶液

存放说明

浓度

免疫原种属

免疫原

纯化方式

偶联

RRID

注意事项

相关讯息

别名

细胞定位

功能与背景

数据库

研究领域

產品图片

GTX113030 IHC-P Image

GTX113030 WB Image

GTX113030 IHC-Wm Image

GTX113030 WB Image

GTX113030 IHC-P Image

GTX113030 WB Image

GTX113030 IHC-P Image

GTX113030 IHC-P Image

GTX113030 WB Image

GTX113030 IHC-P Image

GTX113030 WB Image

GTX113030 WB Image

GTX113030 WB Image

GTX113030 WB Image

GTX113030 WB Image

GTX113030 WB Image

GTX113030 WB Image

文献引用

Application Reference

产品评论

VCP antibody [N1N2], N-term 目录号 GTX113030

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