产品应用
应用说明
*最佳稀释倍数与浓度应由研究人员确认
| 实验应用 |
稀释倍数 |
| Assay dependent |
| Assay dependent |
| 1:100-1:1000 |
| Assay dependent |
WB: 免疫印迹
ICC/IF: 细胞染色
IHC-P: 石蜡切片
IHC-Fr: 冰冻切片
预期分子量
预测种属反应
Xenopus tropicalis, Rhesus Monkey(>80% identity)
产品属性
形式
Liquid
存储溶液
PBS
存放说明
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
浓度
1 mg/ml (Please refer to the vial label for the specific concentration.)
免疫原种属
Human
免疫原
Recombinant protein encompassing a sequence within the center region of human VGAT. The exact sequence is proprietary.
纯化方式
Affinity purified by Protein A.
偶联
Unconjugated
注意事项
仅供实验室使用。不适用于人类或动物的任何临床，治疗或诊断用途。不适合动物或人类食用。
相关讯息
别名
solute carrier family 32 member 1 , VGAT , VIAAT
功能与背景
The protein encoded by this gene is an integral membrane protein involved in gamma-aminobutyric acid (GABA) and glycine uptake into synaptic vesicles. The encoded protein is a member of amino acid/polyamine transporter family II. [provided by RefSeq, Jul 2008]
数据库
研究领域
產品图片
![Anti-VGAT antibody [HL1616] used in IHC (Frozen sections) (IHC-Fr). GTX637107](/templates/default/js/lightbox/images/loading.gif "Anti-VGAT antibody [HL1616] used in IHC (Frozen sections) (IHC-Fr). GTX637107")
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GTX637107 IHC-Fr Image
VGAT antibody [HL1616] detects VGAT protein by immunohistochemical analysis.Sample: Frozen-sectioned mouse cerebellum.Green: VGAT stained by VGAT antibody [HL1616] (GTX637107) diluted at 1:100.
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GTX637107 ICC/IF Image
VGAT antibody [HL1616] detects VGAT protein at nucleus and cytoskeleton by immunofluorescent analysis.Sample: DIV9 rat E18 primary cortical neuron cells were fixed in 4% paraformaldehyde at RT for 15 min.Green: VGAT stained by VGAT antibody [HL1616] (GTX637107) diluted at 1:250.Red: Tau, an axon marker, stained by Tau antibody [GT287] (GTX634809) diluted at 1:500.Blue: Fluoroshield with DAPI (GTX30920).
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GTX637107 IHC-P Image
VGAT antibody [HL1616] detects VGAT protein at cytoplasm by immunohistochemical analysis.Sample: Paraffin-embedded mouse cerebellum.VGAT stained by VGAT antibody [HL1616] (GTX637107) diluted at 1:100.Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
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GTX637107 IHC-Fr Image
VGAT antibody [HL1616] detects VGAT protein at cell membrane by immunohistochemical analysis.Sample: Frozen-sectioned mouse cerebellum.Green: VGAT stained by VGAT antibody [HL1616] (GTX637107) diluted at 1:100.Blue: Fluoroshield with DAPI (GTX30920).
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GTX637107 WB Image
Non-transfected (–) and transfected (+) Boiled and unboiled 293T whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with VGAT antibody [HL1616] (GTX637107) diluted at 1:50000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
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GTX637107 WB Image
Boiled and unboiled mouse tissue extract (50 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with VGAT antibody [HL1616] (GTX637107) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
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GTX637107 IHC-P Image
VGAT antibody [HL1616] detects VGAT protein by immunohistochemical analysis.Sample: Paraffin-embedded mouse eye.Green: VGAT stained by VGAT antibody [HL1616] (GTX637107) diluted at 1:100.Red: beta Tubulin 3/ Tuj1, a Cytoskeleton marker, stained by beta Tubulin 3/ Tuj1 antibody [GT11710] (GTX631836) diluted at 1:500.Blue: Fluoroshield with DAPI (GTX30920).Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
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GTX637107 IHC-P Image
VGAT antibody [HL1616] detects VGAT protein by immunohistochemical analysis.Sample: Paraffin-embedded mouse brain.Green: VGAT stained by VGAT antibody [HL1616] (GTX637107) diluted at 1:100.Red: NF-H, a Cytoplasm marker, stained by NF-H antibody [GT114] (GTX634289) diluted at 1:500.Blue: Fluoroshield with DAPI (GTX30920).Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
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文献引用
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产品评论
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![Anti-VGAT antibody [HL1616] used in IHC (Frozen sections) (IHC-Fr). GTX637107](/upload/website/prouct_img/small/GTX637107/GTX637107_T-44732_20221202_IHC-Fr_M_22121123_949.webp "Anti-VGAT antibody [HL1616] used in IHC (Frozen sections) (IHC-Fr). GTX637107")
![Anti-VGAT antibody [HL1616] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX637107](/upload/website/prouct_img/small/GTX637107/GTX637107_44788_20221209_ICC_IF_R_22122018_363.webp "Anti-VGAT antibody [HL1616] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX637107")
![Anti-VGAT antibody [HL1616] used in IHC (Paraffin sections) (IHC-P). GTX637107](/upload/website/prouct_img/small/GTX637107/GTX637107_T-44732_20220805_IHC-P_M_22080923_105.webp "Anti-VGAT antibody [HL1616] used in IHC (Paraffin sections) (IHC-P). GTX637107")
![Anti-VGAT antibody [HL1616] used in IHC (Frozen sections) (IHC-Fr). GTX637107](/upload/website/prouct_img/small/GTX637107/GTX637107_44788_20230526_IHC-Fr_M_23053001_291.webp "Anti-VGAT antibody [HL1616] used in IHC (Frozen sections) (IHC-Fr). GTX637107")
![Anti-VGAT antibody [HL1616] used in Western Blot (WB). GTX637107](/upload/website/prouct_img/small/GTX637107/GTX637107_44788_20220902_WB_B_22092119_541.webp "Anti-VGAT antibody [HL1616] used in Western Blot (WB). GTX637107")
![Anti-VGAT antibody [HL1616] used in Western Blot (WB). GTX637107](/upload/website/prouct_img/small/GTX637107/GTX637107_T-44732_20221014_WB_M_brain_22101718_856.webp "Anti-VGAT antibody [HL1616] used in Western Blot (WB). GTX637107")
![Anti-VGAT antibody [HL1616] used in IHC (Paraffin sections) (IHC-P). GTX637107](/upload/website/prouct_img/small/GTX637107/GTX637107_44788_20221007_IHC-P_M_1_22110201_204.webp "Anti-VGAT antibody [HL1616] used in IHC (Paraffin sections) (IHC-P). GTX637107")
![Anti-VGAT antibody [HL1616] used in IHC (Paraffin sections) (IHC-P). GTX637107](/upload/website/prouct_img/small/GTX637107/GTX637107_44788_20221007_IHC-P_M_22110201_109.webp "Anti-VGAT antibody [HL1616] used in IHC (Paraffin sections) (IHC-P). GTX637107")
![Anti-VGAT antibody [HL1616] used in IHC (Frozen sections) (IHC-Fr). GTX637107](/upload/website/prouct_img/normal/GTX637107/GTX637107_T-44732_20221202_IHC-Fr_M_22121123_949.webp "Anti-VGAT antibody [HL1616] used in IHC (Frozen sections) (IHC-Fr). GTX637107")
![Anti-VGAT antibody [HL1616] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX637107](/upload/website/prouct_img/normal/GTX637107/GTX637107_44788_20221209_ICC_IF_R_22122018_363.webp "Anti-VGAT antibody [HL1616] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX637107")
![Anti-VGAT antibody [HL1616] used in IHC (Paraffin sections) (IHC-P). GTX637107](/upload/website/prouct_img/normal/GTX637107/GTX637107_T-44732_20220805_IHC-P_M_22080923_105.webp "Anti-VGAT antibody [HL1616] used in IHC (Paraffin sections) (IHC-P). GTX637107")
![Anti-VGAT antibody [HL1616] used in IHC (Frozen sections) (IHC-Fr). GTX637107](/upload/website/prouct_img/normal/GTX637107/GTX637107_44788_20230526_IHC-Fr_M_23053001_291.webp "Anti-VGAT antibody [HL1616] used in IHC (Frozen sections) (IHC-Fr). GTX637107")
![Anti-VGAT antibody [HL1616] used in Western Blot (WB). GTX637107](/upload/website/prouct_img/normal/GTX637107/GTX637107_44788_20220902_WB_B_22092119_541.webp "Anti-VGAT antibody [HL1616] used in Western Blot (WB). GTX637107")
![Anti-VGAT antibody [HL1616] used in Western Blot (WB). GTX637107](/upload/website/prouct_img/normal/GTX637107/GTX637107_T-44732_20221014_WB_M_brain_22101718_856.webp "Anti-VGAT antibody [HL1616] used in Western Blot (WB). GTX637107")
![Anti-VGAT antibody [HL1616] used in IHC (Paraffin sections) (IHC-P). GTX637107](/upload/website/prouct_img/normal/GTX637107/GTX637107_44788_20221007_IHC-P_M_1_22110201_204.webp "Anti-VGAT antibody [HL1616] used in IHC (Paraffin sections) (IHC-P). GTX637107")
![Anti-VGAT antibody [HL1616] used in IHC (Paraffin sections) (IHC-P). GTX637107](/upload/website/prouct_img/normal/GTX637107/GTX637107_44788_20221007_IHC-P_M_22110201_109.webp "Anti-VGAT antibody [HL1616] used in IHC (Paraffin sections) (IHC-P). GTX637107")
