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VIP抗体

Anti-VIP antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX129461
Anti-VIP antibody used in Western Blot (WB). GTX129461
Anti-VIP antibody used in IHC (Frozen sections) (IHC-Fr). GTX129461
Anti-VIP antibody used in IHC (Paraffin sections) (IHC-P). GTX129461
Anti-VIP antibody used in IHC (Paraffin sections) (IHC-P). GTX129461
Anti-VIP antibody used in Western Blot (WB). GTX129461
Anti-VIP antibody used in Western Blot (WB). GTX129461

目录号 GTX129461

文献引用 4

宿主

 Rabbit

克隆

 Polyclonal

同种型

 IgG

实验应用

 WB, ICC/IF, IHC-P, IHC-Fr

种属反应

 Human, Mouse, Rat
产品包装
100 μl (¥4000),
25 μl (¥1700)
查看所有 VIP 的产品

产品应用

应用说明

*最佳稀释倍数与浓度应由研究人员确认
实验应用 稀释倍数
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
IHC-Fr 1:100-1:1000
WB: 免疫印迹
ICC/IF: 细胞染色
IHC-P: 石蜡切片
IHC-Fr: 冰冻切片

预期分子量

19 kDa. ( Note )

阳性对照

PC-3

预测种属反应

Zebrafish, Sheep, Bovine, Pig, Rhesus Monkey(>80% identity)

产品属性

形式

Liquid

存储溶液

PBS, 20% Glycerol

存放说明

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

浓度

1.42 mg/ml (Please refer to the vial label for the specific concentration.)

免疫原种属

Human

免疫原

Carrier-protein conjugated synthetic peptide encompassing a sequence within the C-terminus region of human VIP. The exact sequence is proprietary.

纯化方式

Purified by antigen-affinity chromatography.

偶联

Unconjugated

RRID

AB_2886003

注意事项

仅供实验室使用。不适用于人类或动物的任何临床，治疗或诊断用途。不适合动物或人类食用。

相关讯息

别名

vasoactive intestinal peptide , PHM27

细胞定位

Secreted

功能与背景

The protein encoded by this gene belongs to the glucagon family. It stimulates myocardial contractility, causes vasodilation, increases glycogenolysis, lowers arterial blood pressure and relaxes the smooth muscle of trachea, stomach and gall bladder. Alternative splicing occurs at this locus and two transcript variants encoding distinct isoforms have been identified. [provided by RefSeq]

数据库

研究领域

產品图片

Anti-VIP antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX129461

GTX129461 ICC/IF Image

VIP antibody detects VIP protein by immunofluorescent analysis.
Sample: DIV9 rat E18 primary hippocampal neuron cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: VIP stained by VIP antibody (GTX129461) diluted at 1:500.
Red: beta Tubulin 3/ Tuj1, stained by beta Tubulin 3/ Tuj1 antibody [GT11710] (GTX631836) diluted at 1:500.
Blue: Fluoroshield with DAPI (GTX30920).

Anti-VIP antibody used in Western Blot (WB). GTX129461

GTX129461 WB Image

VIP antibody detects VIP protein by western blot analysis. Whole cell extracts (30 μg) was separated by 15% SDS-PAGE, and the membrane was blotted with VIP antibody (GTX129461) at a dilution of 1:1000.

Anti-VIP antibody used in IHC (Frozen sections) (IHC-Fr). GTX129461

GTX129461 IHC-Fr Image

VIP antibody detects VIP protein by immunohistochemical analysis.
Sample: Frozen-sectioned mouse thalamus.
Green: VIP stained by VIP antibody (GTX129461) diluted at 1:250.
Blue: Fluoroshield with DAPI (GTX30920).

Anti-VIP antibody used in IHC (Paraffin sections) (IHC-P). GTX129461

GTX129461 IHC-P Image

VIP antibody detects VIP protein at cytoplasm by immunohistochemical analysis.
Sample: Paraffin-embedded mouse pancreas.
VIP stained by VIP antibody (GTX129461) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-VIP antibody used in IHC (Paraffin sections) (IHC-P). GTX129461

GTX129461 IHC-P Image

VIP antibody detects VIP protein at cytoplasm by immunohistochemical analysis.
Sample: Paraffin-embedded rat pancreas.
VIP stained by VIP antibody (GTX129461) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-VIP antibody used in Western Blot (WB). GTX129461

GTX129461 WB Image

Various whole cell extracts (30 μg) were separated by 15% SDS-PAGE, and the membrane was blotted with VIP antibody (GTX129461) diluted at 1:3000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-VIP antibody used in Western Blot (WB). GTX129461

GTX129461 WB Image

Rat tissue extracts (50 μg) was separated by 15% SDS-PAGE, and the membrane was blotted with VIP antibody (GTX129461) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

文献引用

Application Reference

Wu CY et al. Biology (Basel) 2023; 12 (10) Klotho Null Mutation Indirectly Leads to Age-Related Lacrimal Gland Degeneration in Mutant Mice.
Broome ST et al. J Mol Neurosci 2023; 73 (9月10日) : 724-737 Early Alterations of PACAP and VIP Expression in the Female Rat Brain Following Spinal Cord Injury.
De-Fong Huang et al. 2022; 119 (33) Neuronal splicing regulator RBFOX3 mediates seizures via regulating Vamp1 expression preferentially in NPY-expressing GABAergic neurons.
实验应用 : IHC-Fr
种属反应 : Mouse
Musumeci G et al. Int J Mol Sci 2018; 19 (4) Identification of Dysregulated microRNA Networks in Schwann Cell-Like Cultures Exposed to Immune Challenge: Potential Crosstalk with the Protective VIP/PACAP Neuropeptide System.
实验应用 : WB
种属反应 : Rat
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SDS
PBS.pdf
Glycerol.pdf
Proclin.pdf
产品包装 报价 (¥)
¥ 4000
¥ 1700
Currently in production
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