产品应用
应用说明
*最佳稀释倍数与浓度应由研究人员确认
实验应用 |
稀释倍数 |
1:500-1:3000 |
Assay dependent |
Assay dependent |
WB: 免疫印迹
ICC/IF: 细胞染色
IHC-P: 石蜡切片
IHC-Fr: 冰冻切片
预期分子量
产品说明
This antibody was raised against human VRL1 Intracellular domain.
产品属性
形式
Liquid
存储溶液
PBS
存放说明
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
浓度
1 mg/ml (Please refer to the vial label for the specific concentration.)
免疫原种属
Human
免疫原
Recombinant protein encompassing a sequence within the Intracellular domain of human VRL1. The exact sequence is proprietary.
纯化方式
Affinity purified by Protein A.
偶联
Unconjugated
注意事项
仅供实验室使用。不适用于人类或动物的任何临床,治疗或诊断用途。不适合动物或人类食用。
相关讯息
别名
transient receptor potential cation channel subfamily V member 2 , VRL , VRL-1 , VRL1
细胞定位
Cell membrane,Cytoplasm,Melanosome
功能与背景
This gene encodes an ion channel that is activated by high temperatures above 52 degrees Celsius. The protein may be involved in transduction of high-temperature heat responses in sensory ganglia. It is thought that in other tissues the channel may be activated by stimuli other than heat. [provided by RefSeq, Jul 2008]
数据库
研究领域
產品图片
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GTX637018 ICC/IF Image
VRL1 antibody [HL1543] detects VRL1 protein at cell membrane and cytoplasm by immunofluorescent analysis.Sample: K562 cells were fixed in 4% paraformaldehyde at RT for 15 min.Green: VRL1 stained by VRL1 antibody [HL1543] (GTX637018) diluted at 1:500.Blue: Fluoroshield with DAPI (GTX30920).
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GTX637018 IHC-P Image
VRL1 antibody [HL1543] detects VRL1 protein by immunohistochemical analysis.Sample: Paraffin-embedded mouse brain.VRL1 stained by VRL1 antibody [HL1543] (GTX637018) diluted at 1:100.Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
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GTX637018 WB Image
Unboiled K562 whole cell and membrane extracts (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with VRL1 antibody [HL1543] (GTX637018) diluted at 1:2000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced. (WCE: whole cell extract; ME: membrane extract)
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GTX637018 ICC/IF Image
VRL1 antibody [HL1543] detects VRL1 protein at fiber by immunofluorescent analysis.Sample: DIV9 rat E18 primary hippocampal neuron cells were fixed in 4% paraformaldehyde at RT for 15 min.Green: VRL1 stained by VRL1 antibody [HL1543] (GTX637018) diluted at 1:250.Blue: Fluoroshield with DAPI (GTX30920).
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