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Vasa抗体

Anti-Vasa antibody used in IHC (Whole mount) (IHC-Wm). GTX128306
Anti-Vasa antibody used in Western Blot (WB). GTX128306
Anti-Vasa antibody used in IHC (Whole mount) (IHC-Wm). GTX128306
Anti-Vasa antibody used in Western Blot (WB). GTX128306
Anti-Vasa antibody used in Western Blot (WB). GTX128306
Anti-Vasa antibody used in IHC (Paraffin sections) (IHC-P). GTX128306

目录号 GTX128306

文献引用 18

宿主

 Rabbit

克隆

 Polyclonal

同种型

 IgG

实验应用

 WB, ICC/IF, IHC-P, IHC-Wm, IHC

种属反应

 Zebrafish, Japanese Medaka, Fish
产品包装
100 μl (¥4000),
25 μl (¥1700)
查看所有 Vasa 的产品

产品应用

应用说明

*最佳稀释倍数与浓度应由研究人员确认
实验应用 稀释倍数
WB 1:500-1:3000
ICC/IF Assay dependent
IHC-P Assay dependent
IHC-Wm 1:100-1:500
IHC Assay dependent
WB: 免疫印迹
ICC/IF: 细胞染色
IHC-P: 石蜡切片
IHC-Fr: 冰冻切片

阳性对照

Zebrafish 10 hpf

产品属性

形式

Liquid

存储溶液

PBS

存放说明

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

浓度

1 mg/ml (Please refer to the vial label for the specific concentration.)

免疫原种属

Zebrafish

免疫原

Recombinant protein encompassing a sequence within the center region of zebrafish Vasa. The exact sequence is proprietary.

纯化方式

Purified by antigen-affinity chromatography.

偶联

Unconjugated

RRID

AB_2847856

注意事项

仅供实验室使用。不适用于人类或动物的任何临床，治疗或诊断用途。不适合动物或人类食用。

相关讯息

别名

DEAD (Asp-Glu-Ala-Asp) box polypeptide 4 , vas , vasa , vlg , wu:fi24g05 , zgc:109812 , zgc:158535

数据库

研究领域

產品图片

Anti-Vasa antibody used in IHC (Whole mount) (IHC-Wm). GTX128306

GTX128306 IHC-Wm Image

vasa antibody detects vasa protein at primordial germ cell on whole-mount zebrafish embryos by immunohistochemical analysis.
Sample: Paraformaldehyde-fixed zebrafish embryos.
vasa antibody (GTX128306) dilution: 1:200.
Anti-Vasa antibody used in Western Blot (WB). GTX128306

GTX128306 WB Image

Whole zebrafish extract (30 μg) was separated by 7.5% SDS-PAGE, and the membrane was blotted with Vasa antibody (GTX128306) diluted at 1:2000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-Vasa antibody used in IHC (Whole mount) (IHC-Wm). GTX128306

GTX128306 IHC-Wm Image

Vasa antibody detects Vasa protein on Medaka by whole mount immunohistochemical analysis.
Sample: 7 days-post-fertilization medaka embryo.
Vasa antibody (GTX128306) dilution: 1:100.
Anti-Vasa antibody used in Western Blot (WB). GTX128306

GTX128306 WB Image

Vasa antibody detects Vasa protein by western blot analysis.
A. 30 μg Zebrafish 10 hpf lysate/extract
7.5% SDS-PAGE
Vasa antibody (GTX128306) dilution: 1:2000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-Vasa antibody used in Western Blot (WB). GTX128306

GTX128306 WB Image

The data was published in the journal PLoS Genet in 2018.PMID: 30399145

Anti-Vasa antibody used in IHC (Paraffin sections) (IHC-P). GTX128306

GTX128306 IHC-P Image

The data was published in the journal Sci Rep in 2017.PMID: 28211534

文献引用

Application Reference

Westerich KJ et al. bioRxiv 2023; Spatial organization and function of RNA molecules within phase-separated condensates are controlled by Dnd1.
Xiaoyun Sun et al. Int J Mol Sci 2022; 23 (11) Isolation and Characterization of Germline Stem Cells in Protogynous Hermaphroditic Monopterus albus.
种属反应 : Zebrafish
Gang Zhai et al. Elife 2022; 11 Augmentation of progestin signaling rescues testis organization and spermatogenesis in zebrafish with the depletion of androgen signaling.
实验应用 : IHC-P
种属反应 : Zebrafish
Stefan Redl et al. Development 2021; 148 (2) Extensive nuclear gyration and pervasive non-genic transcription during primordial germ cell development in zebrafish.
实验应用 : IHC-Wm
种属反应 : Zebrafish
Raquel Atienzar-Aroca et al. Biology (Basel) 2021; 10 (2) Knockout of myoc Provides Evidence for the Role of Myocilin in Zebrafish Sex Determination Associated with Wnt Signalling Downregulation.
实验应用 : IHC-Wm
种属反应 : Zebrafish
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Application Reference
Westerich KJ et al. bioRxiv 2023; Spatial organization and function of RNA molecules within phase-separated condensates are controlled by Dnd1.
Xiaoyun Sun et al. Int J Mol Sci 2022; 23 (11) Isolation and Characterization of Germline Stem Cells in Protogynous Hermaphroditic Monopterus albus.
种属反应 : Zebrafish
Gang Zhai et al. Elife 2022; 11 Augmentation of progestin signaling rescues testis organization and spermatogenesis in zebrafish with the depletion of androgen signaling.
实验应用 : IHC-P
种属反应 : Zebrafish
Stefan Redl et al. Development 2021; 148 (2) Extensive nuclear gyration and pervasive non-genic transcription during primordial germ cell development in zebrafish.
实验应用 : IHC-Wm
种属反应 : Zebrafish
Raquel Atienzar-Aroca et al. Biology (Basel) 2021; 10 (2) Knockout of myoc Provides Evidence for the Role of Myocilin in Zebrafish Sex Determination Associated with Wnt Signalling Downregulation.
实验应用 : IHC-Wm
种属反应 : Zebrafish
Hansen C.L. et al. Genesis. 2021; 59 (10):e23452 Conserved germ plasm characteristics across the Danio and Devario lineages.
实验应用 : ICC/IF
种属反应 : Zebrafish
Higaki S et al. J Fish Biol 2021; (Epub) Germ cell-specific expression of Venus by Tol2-mediated transgenesis in endangered endemic cyprinid Honmoroko (Gnathopogon caerulescens).
Lai F et al. Int J Biol Sci 2021; 17 (8):2009-2020 Identification of Histone Modifications Reveals a Role of H2b Monoubiquitination in Transcriptional Regulation of dmrt1 in Monopterus albus.
种属反应 : Fish
Dedukh D et al. Sci Rep 2020; 10 (1):8720 Micronuclei in germ cells of hybrid frogs from Pelophylax esculentus complex contain gradually eliminated chromosomes.
实验应用 : IHC-Wm
Ye D et al. Mar Biotechnol (NY) 2019; 21 (2):217-228 Abundance of Early Embryonic Primordial Germ Cells Promotes Zebrafish Female Differentiation as Revealed?by Lifetime Labeling of Germline.
Yan Zhao et al. Aquaculture 2019; 515:734535 Gonad development examination of high-temperature–treated genetically female Nile tilapia.
实验应用 : IHC-P
种属反应 : Fish
Zhang X et al. PLoS One 2019; 14 (8):e0220364 Transcriptomic profile of early zebrafish PGCs by single cell sequencing.
实验应用 : IHC-Wm
Krishnakumar P et al. PLoS Genet 2018; 14 (11):e1007696 Functional equivalence of germ plasm organizers.
实验应用 : WB
种属反应 : Zebrafish
Higaki S et al. Fish Physiol Biochem 2017; (Epub) Cryopreservation of male and female gonial cells by vitrification in the critically endangered cyprinid honmoroko Gnathopogon caerulescens.
Higaki S et al. Zygote 2017;:43110 Successful vitrification of whole juvenile testis in the critically endangered cyprinid honmoroko (Gnathopogon caerulescens).
实验应用 : IHC-P
种属反应 : Zebrafish
Liu Y et al. Biochimie 2017; (Epub) Nanog suppresses the expression of vasa by directly regulating nlk1 in the early zebrafish embryo.
实验应用 : WB
种属反应 : Zebrafish
Higaki S et al. Sci Rep 2017; 7:42852 In vitro differentiation of fertile sperm from cryopreserved spermatogonia of the endangered endemic cyprinid honmoroko (Gnathopogon caerulescens).
实验应用 : IHC-P
种属反应 : Zebrafish
Shen J et al. Biochem Biophys Res Commun 2016; 478 (2):858-63 Diethylnitrosamine-induced expression of germline-specific genes and pluripotency factors, including vasa and oct4, in medaka somatic cells.
实验应用 : IHC
种属反应 : Zebrafish
SDS
PBS.pdf
Glycerol.pdf
Thimerosal.pdf
产品包装 报价 (¥)
¥ 4000
¥ 1700
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