产品应用
应用说明
*最佳稀释倍数与浓度应由研究人员确认
| 实验应用 |
稀释倍数 |
| Assay dependent |
| Assay dependent |
WB: 免疫印迹
ICC/IF: 细胞染色
IHC-P: 石蜡切片
IHC-Fr: 冰冻切片
预期分子量
产品说明
This antibody is specific for human Villin protein, and it does not cross react with human Advillin, Gelsolin, and Villin like protein.
产品属性
形式
Liquid
存储溶液
PBS
存放说明
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
浓度
0.5 mg/ml (Please refer to the vial label for the specific concentration.)
免疫原种属
Human
免疫原
Recombinant fragmemt of human Villin
纯化方式
Affinity purified by Protein A.
偶联
Unconjugated
注意事项
仅供实验室使用。不适用于人类或动物的任何临床，治疗或诊断用途。不适合动物或人类食用。
相关讯息
别名
villin 1 , D2S1471 , VIL
细胞定位
Cytoplasm, cytoskeleton,Cell projection, lamellipodium,Cell projection, filopodium
功能与背景
This gene encodes a member of a family of calcium-regulated actin-binding proteins. This protein represents a dominant part of the brush border cytoskeleton which functions in the capping, severing, and bundling of actin filaments. Two mRNAs of 2.7 kb and 3.5 kb have been observed; they result from utilization of alternate poly-adenylation signals present in the terminal exon. [provided by RefSeq, Jul 2008]
数据库
研究领域
產品图片
![Anti-Villin antibody [HL1830] used in Western Blot (WB). GTX637555](/templates/default/js/lightbox/images/loading.gif "Anti-Villin antibody [HL1830] used in Western Blot (WB). GTX637555")
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GTX637555 WB Image
Whole cell extract (30 μg) was separated by 7.5% SDS-PAGE, and the membranes were blotted with Villin antibody [HL1830] (GTX637555) diluted at 1:4000 and competitor's antibody (#Highly competitor antibody) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
*The competitor is not affiliated with GeneTex and does not endorse this product.
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GTX637555 WB Image
Non-transfected (–) and transfected (+) 293T whole cell extracts were separated by 7.5% SDS-PAGE, and the membrane was blotted with Villin antibody [HL1830] (GTX637555) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
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GTX637555 IHC-P Image
Villin antibody [HL1830] detects Villin protein at cytoplasm by immunohistochemical analysis.Sample: Paraffin-embedded mouse duodenum.Villin stained by Villin antibody [HL1830] (GTX637555) diluted at 1:100.Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
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GTX637555 WB Image
Various whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with Villin antibody [HL1830] (GTX637555) diluted at 1:4000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody. Corresponding RNA expression data for the same cell lines are based on Human Protein Atlas program.
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文献引用
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产品评论
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![Anti-Villin antibody [HL1830] used in Western Blot (WB). GTX637555](/upload/website/prouct_img/small/GTX637555/GTX637555_44970_20230707_WB_competitor_watermark_23071300_644.webp "Anti-Villin antibody [HL1830] used in Western Blot (WB). GTX637555")
![Anti-Villin antibody [HL1830] used in Western Blot (WB). GTX637555](/upload/website/prouct_img/small/GTX637555/GTX637555_T-44823_20230210_WB_multiple_B_23021401_696.webp "Anti-Villin antibody [HL1830] used in Western Blot (WB). GTX637555")
![Anti-Villin antibody [HL1830] used in IHC (Paraffin sections) (IHC-P). GTX637555](/upload/website/prouct_img/small/GTX637555/GTX637555_T-44823_20230714_IHC-P_M_23080901_576.webp "Anti-Villin antibody [HL1830] used in IHC (Paraffin sections) (IHC-P). GTX637555")
![Anti-Villin antibody [HL1830] used in Western Blot (WB). GTX637555](/upload/website/prouct_img/small/GTX637555/GTX637555_44970_20230317_WB_TPM_watermark_23032022_712.webp "Anti-Villin antibody [HL1830] used in Western Blot (WB). GTX637555")
![Anti-Villin antibody [HL1830] used in Western Blot (WB). GTX637555](/upload/website/prouct_img/normal/GTX637555/GTX637555_44970_20230707_WB_competitor_watermark_23071300_644.webp "Anti-Villin antibody [HL1830] used in Western Blot (WB). GTX637555")
![Anti-Villin antibody [HL1830] used in Western Blot (WB). GTX637555](/upload/website/prouct_img/normal/GTX637555/GTX637555_T-44823_20230210_WB_multiple_B_23021401_696.webp "Anti-Villin antibody [HL1830] used in Western Blot (WB). GTX637555")
![Anti-Villin antibody [HL1830] used in IHC (Paraffin sections) (IHC-P). GTX637555](/upload/website/prouct_img/normal/GTX637555/GTX637555_T-44823_20230714_IHC-P_M_23080901_576.webp "Anti-Villin antibody [HL1830] used in IHC (Paraffin sections) (IHC-P). GTX637555")
![Anti-Villin antibody [HL1830] used in Western Blot (WB). GTX637555](/upload/website/prouct_img/normal/GTX637555/GTX637555_44970_20230317_WB_TPM_watermark_23032022_712.webp "Anti-Villin antibody [HL1830] used in Western Blot (WB). GTX637555")
