Vimentin抗体 [GT1180]

Name: Vimentin抗体 [GT1180]
Brand: GeneTex
SKU: GTX00942
Price: 5000.00 RMB

Anti-Vimentin antibody [GT1180] used in Western Blot (WB). GTX00942

Anti-Vimentin antibody [GT1180] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX00942

Anti-Vimentin antibody [GT1180] used in IHC (Paraffin sections) (IHC-P). GTX00942

Anti-Vimentin antibody [GT1180] used in Immunoprecipitation (IP). GTX00942

目录号 GTX00942

文献引用 1

宿主	Rabbit
克隆	Monoclonal
克隆编号	GT1180
同种型	IgG
实验应用	WB, ICC/IF, IHC-P, IP
种属反应	Human, Mouse, Rat
产品包装	100 μl (¥5000)

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View product citations for antibody GTX00942 on CiteAb

产品应用

应用说明

*最佳稀释倍数与浓度应由研究人员确认

实验应用	稀释倍数
WB	1:500 - 1:2000
ICC/IF	1:50 - 1:200
IHC-P	1:50 - 1:200
IP	1:50 - 1:200

WB: 免疫印迹
ICC/IF: 细胞染色
IHC-P: 石蜡切片
IHC-Fr: 冰冻切片

预期分子量

54 kDa. ( Note )

产品属性

形式

Liquid

存储溶液

PBS, 50% Glycerol

存放说明

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

浓度

Batch dependent (Please refer to the vial label for the specific concentration.)

免疫原种属

Human

免疫原

A synthesized peptide derived from human Vimentin.

纯化方式

Purified by affinity chromatography

偶联

Unconjugated

注意事项

仅供实验室使用。不适用于人类或动物的任何临床，治疗或诊断用途。不适合动物或人类食用。

别名

vimentin , CTRCT30 , HEL113 , VIM , Vimentin

细胞定位

Cytoplasm

功能与背景

This gene encodes a type III intermediate filament protein. Intermediate filaments, along with microtubules and actin microfilaments, make up the cytoskeleton. The encoded protein is responsible for maintaining cell shape and integrity of the cytoplasm, and stabilizing cytoskeletal interactions. This protein is involved in neuritogenesis and cholesterol transport and functions as an organizer of a number of other critical proteins involved in cell attachment, migration, and signaling. Bacterial and viral pathogens have been shown to attach to this protein on the host cell surface. Mutations in this gene are associated with congenital cataracts in human patients. [provided by RefSeq, Aug 2017]

数据库

Gene ID: 7431 VIM
UniProt: P08670 VIM

研究领域

產品图片

GTX00942 WB Image

Various whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with Vimentin antibody [GT1180] (GTX00942) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody. Corresponding RNA expression data for the same cell lines are based on Human Protein Atlas program.

GTX00942 WB Image

Various whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with Vimentin antibody [GT1180] (GTX00942) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
The observed M.W. is based on the publication: PMID: 23185433

GTX00942 ICC/IF Image

ICC/IF analysis of PFA-fixed HeLa cells using GTX00942 Vimentin antibody [GT1180].
Orange : Primary antibody
Blue : DAPI
Dilution : 1:100

GTX00942 WB Image

WB analysis of various samples using GTX00942 Vimentin antibody [GT1180].
Dilution : 1:1000
Loading : 25μg

GTX00942 IHC-P Image

IHC-P analysis of Human colon carcinoma tissue section using GTX00942 Vimentin antibody [GT1180].
Dilution : 1:100

GTX00942 ICC/IF Image

ICC/IF analysis of PFA-fixed NIH-3T3 cells using GTX00942 Vimentin antibody [GT1180].
Orange : Primary antibody
Blue : DAPI
Dilution : 1:100

GTX00942 ICC/IF Image

ICC/IF analysis of PFA-fixed C6 cells using GTX00942 Vimentin antibody [GT1180].
Orange : Primary antibody
Blue : DAPI
Dilution : 1:100

GTX00942 IP Image

IP analysis of Jurkat cell lysate using GTX00942 Vimentin antibody [GT1180].
Antibody amount : 3μg / 300μg lysate
Dilution : 1:1000

GTX00942 WB Image

WB analysis of wild-type (WT) and Vimentin knockout (KO) 293T cell extracts using GTX00942 Vimentin antibody [GT1180].
Dilution : 1:1000
Laoding : 25μg

GTX00942 IHC-P Image

IHC-P analysis of Rat spleen tissue section using GTX00942 Vimentin antibody [GT1180].
Dilution : 1:100

GTX00942 IHC-P Image

IHC-P analysis of Mouse kidney tissue section using GTX00942 Vimentin antibody [GT1180].
Dilution : 1:100

文献引用

Application Reference

Meng-Hui Zhang et al. Bioengineered 2022; 13 (4) : 10047-10060 Cleavage stimulation factor 2 promotes malignant progression of liver hepatocellular carcinoma by activating phosphatidylinositol 3'-kinase/protein kinase B/mammalian target of rapamycin pathway.

实验应用 : WB

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Application Reference

Meng-Hui Zhang et al. Bioengineered 2022; 13 (4):10047-10060 Cleavage stimulation factor 2 promotes malignant progression of liver hepatocellular carcinoma by activating phosphatidylinositol 3'-kinase/protein kinase B/mammalian target of rapamycin pathway.

实验应用 : WB

SDS
PBS.pdf
Glycerol.pdf
Sodium Azide.pdf

产品包装

报价 (¥)

100 μl

¥ 5000

Qty

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Vimentin抗体 [GT1180]

目录号 GTX00942

宿主

克隆

克隆编号

同种型

实验应用

种属反应

产品应用

应用说明

预期分子量

产品属性

形式

存储溶液

存放说明

浓度

免疫原种属

免疫原

纯化方式

偶联

注意事项

相关讯息

别名

细胞定位

功能与背景

数据库

研究领域

產品图片

GTX00942 WB Image

GTX00942 WB Image

GTX00942 ICC/IF Image

GTX00942 WB Image

GTX00942 IHC-P Image

GTX00942 ICC/IF Image

GTX00942 ICC/IF Image

GTX00942 IP Image

GTX00942 WB Image

GTX00942 IHC-P Image

GTX00942 IHC-P Image

文献引用

Application Reference

产品评论

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