Vimentin抗体 [GT812]

Name: Vimentin抗体 [GT812]
Brand: GeneTex
SKU: GTX629744
Price: 4000.00 RMB
Rating: 5 (1 reviews)

Anti-Vimentin antibody [GT812] used in IHC (Frozen sections) (IHC-Fr). GTX629744

Anti-Vimentin antibody [GT812] used in Western Blot (WB). GTX629744

Anti-Vimentin antibody [GT812] used in IHC (Paraffin sections) (IHC-P). GTX629744

Anti-Vimentin antibody [GT812] used in ELISA (ELISA). GTX629744

目录号 GTX629744

文献引用 9

宿主	Mouse
克隆	Monoclonal
克隆编号	GT812
同种型	IgG2b
实验应用	WB, ICC/IF, IHC-P, IHC-Fr, FACS, IP, ELISA, Sandwich ELISA
种属反应	Human, Mouse, Rat, Cat
产品包装	100 μl (¥4000), 25 μl (¥1700)

查看所有 Vimentin 的产品

View product citations for antibody GTX629744 on CiteAb

产品应用

应用说明

*最佳稀释倍数与浓度应由研究人员确认

实验应用	稀释倍数
WB	1:500-1:75000
ICC/IF	Assay dependent
IHC-P	1:100-1:1000
IHC-Fr	1:100-1:1000
FACS	Assay dependent
IP	Assay dependent
ELISA	Assay dependent
Sandwich ELISA	Assay dependent
Note : Sandwich ELISA Capture : GTX629744, Detection : GTX112661 / GTX100619

WB: 免疫印迹
ICC/IF: 细胞染色
IHC-P: 石蜡切片
IHC-Fr: 冰冻切片

预期分子量

54 kDa. ( Note )

阳性对照

293T , HeLa , U87-MG , SK-N-SH , IMR32 , SK-N-AS , Rat-2 , Neuro2A , C8D30 , NIH-3T3 , C2C12

产品说明

IP/MS validation was supported by references (PMID:30377427)

预测种属反应

Bovine, Dog, Chicken, Guinea pig, Xenopus laevis, Chimpanzee(>80% identity)

产品属性

形式

Liquid

存储溶液

PBS

存放说明

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

浓度

1 mg/ml (Please refer to the vial label for the specific concentration.)

免疫原种属

Human

免疫原

Recombinant protein encompassing a sequence within the center region of human Vimentin. The exact sequence is proprietary.

纯化方式

Affinity purified by Protein G.

偶联

Unconjugated

RRID

AB_2814972

注意事项

仅供实验室使用。不适用于人类或动物的任何临床，治疗或诊断用途。不适合动物或人类食用。

别名

vimentin

功能与背景

Along with the microfilaments (actins) and microtubules (tubulins), the intermediate filaments represent a third class of well-characterized cytoskeletal elements. The subunits display a tissue-specific pattern of expression. Desmin (MIM 125660) is the subunit specific for muscle and vimentin the subunit specific for mesenchymal tissue.[supplied by OMIM]

数据库

Gene ID: 7431 VIM
UniProt: P08670 VIM

研究领域

產品图片

GTX629744 IHC-Fr Image

Vimentin antibody [GT812] detects Vimentin proteins in embryonic mouse brain by immunohistochemical analysis.
Sample: Frozen section of embryonic mouse brain (mE18.5).
Red: Vimentin antibody [GT812] (GTX629744) diluted at 1:250.
Blue: DAPI.

GTX629744 WB Image

Wild-type (WT) and Vimentin knockout (KO) 293T cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with Vimentin antibody [GT812] (GTX629744) diluted at 1:5000. The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody.

GTX629744 WB Image

Various whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membranes were blotted with Vimentin antibody [GT812] (GTX629744) diluted at 1:5000 and competitor's antibody diluted at 1:5000. The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody.

*The competitor is not affiliated with GeneTex and does not endorse this product.

GTX629744 WB Image

Various whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with Vimentin antibody [GT812] (GTX629744) diluted at 1:3000.

GTX629744 WB Image

Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with Vimentin antibody [GT812] (GTX629744) diluted at 1:5000.

GTX629744 IHC-P Image

Vimentin antibody [GT812] detects Vimentin protein at cytoplasm in rat ovary by immunohistochemical analysis.
Sample: Paraffin-embedded rat ovary.
Vimentin antibody [GT812] (GTX629744) diluted at 1:250.

Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

GTX629744 WB Image

Whole cell extract (30 μg) was separated by 10% SDS-PAGE, and the membrane was blotted with Vimentin antibody [GT812] (GTX629744) diluted at 1:75000.

GTX629744 ELISA Image

Sandwich ELISA detection of recombinant full-length Vimentin protein using GTX629744 as capture antibody at concentration of 5 μg/mL and GTX100619 as detection antibody at concentration of 1 μg/mL. Rabbit IgG antibody (HRP) (GTX213110-01) was diluted at 1:10000 and used to detect the primary antibody.

GTX629744 WB Image

Various whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with Vimentin antibody [GT812] (GTX629744) diluted at 1:5000.

GTX629744 WB Image

Non-transfected (–) and transfected (+) 293T whole cell extracts (10 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with Vimentin antibody [GT812] (GTX629744) diluted at 1:10000. The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody.

GTX629744 IHC-Fr Image

Vimentin antibody [GT812] detects Vimentin protein expression by immunohistochemical analysis.
Sample: Frozen sectioned E13.5 Rat brain.
Green: Vimentin protein stained by Vimentin antibody [GT812] (GTX629744) diluted at 1:250.
Red: beta Tubulin 3/ TUJ1, a mature neuron marker, stained by beta Tubulin 3/ TUJ1 antibody (GTX130245) diluted at 1:250.
Blue: Fluoroshield with DAPI (GTX30920).

GTX629744 ELISA Image

Sandwich ELISA detection of recombinant full-length Vimentin protein using GTX629744 as capture antibody at concentration of 5 μg/mL and GTX112661 as detection antibody at concentration of 1 μg/mL. Rabbit IgG antibody (HRP) (GTX213110-01) was diluted at 1:10000 and used to detect the primary antibody.

GTX629744 WB Image

Vimentin antibody [GT812] detects Vimentin protein by Western blot analysis.
A. 30 μg 293T whole cell lysate/extract
B. 30 μg HeLa whole cell lysate/extract
10 % SDS-PAGE
Vimentin antibody [GT812] (GTX629744) dilution: 1:1000

GTX629744 WB Image

Whole cell extract (30 μg) was separated by 10% SDS-PAGE, and the membrane was blotted with Vimentin antibody (GTX629744) diluted at 1:500000. The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.

文献引用

Application Reference

G Hurtado-Alvarado et al. Curr Biol 2023; S0960-9822 (23) : 01151-X Suprachiasmatic nucleus promotes hyperglycemia induced by sleep delay

Yang KS et al. Sci Rep 2023; 13 (1) : 10969 Extracellular vesicle analysis of plasma allows differential diagnosis of atypical pancreatic serous cystadenoma.

Wanandi SI et al. J Pak Med Assoc 2021; 71(Suppl 2) (2) : S18-S24 Cancer-associated fibroblast (CAF) secretomes-induced epithelial-mesenchymal transition on HT-29 colorectal carcinoma cells associated with hepatocyte growth factor (HGF) signalling.

实验应用 : ICC/IF

种属反应 : Human

Liu L et al. Mol Cancer Ther 2019; (Epub)

Lee SW et al. Biosens Bioelectron 2019; 142 : 111509 Dual-function nanostructured platform for isolation of nasopharyngeal carcinoma circulating tumor cells and EBV DNA detection.

实验应用 : ICC/IF

种属反应 : Human

显示更多 +

我要回报文献

产品评论

Vimentin antibody [GT812] 目录号 GTX629744

Rating

( Average 5 based on 1 users reviews)

实验应用

Flow cytometry(FACS)

( Average 5 based on 1 users reviews)

Date : Anonymous submitted on 26-Sep-2016

Score :

Application Tested : FACS

Sample Species : Hu

Sample : HT29 cells

Blocking : 1% skim milk, 4°C, 1Hr

Fixation : 70% EtOH, -20°C, 1Hr

Permeabilization /Antigen retrieval : 70% EtOH, -20°C, 1hrHr

Primary Antibody : 1:200, 4°C, 1Hr

我要评论产品

Application Reference

G Hurtado-Alvarado et al. Curr Biol 2023; S0960-9822 (23):01151-X Suprachiasmatic nucleus promotes hyperglycemia induced by sleep delay

Yang KS et al. Sci Rep 2023; 13 (1):10969 Extracellular vesicle analysis of plasma allows differential diagnosis of atypical pancreatic serous cystadenoma.

Wanandi SI et al. J Pak Med Assoc 2021; 71(Suppl 2) (2):S18-S24 Cancer-associated fibroblast (CAF) secretomes-induced epithelial-mesenchymal transition on HT-29 colorectal carcinoma cells associated with hepatocyte growth factor (HGF) signalling.

实验应用 : ICC/IF

种属反应 : Human

Liu L et al. Mol Cancer Ther 2019; (Epub)

Lee SW et al. Biosens Bioelectron 2019; 142:111509 Dual-function nanostructured platform for isolation of nasopharyngeal carcinoma circulating tumor cells and EBV DNA detection.

实验应用 : ICC/IF

种属反应 : Human

Sikorski K et al. Nat Methods 2018; 15 (11):909-912 A high-throughput pipeline for validation of antibodies.

实验应用 : IP

种属反应 : Human

Liu MC et al. PLoS One 2017; 12 (5):e0178152 Inhibition of chronic prostate inflammation by hyaluronic acid through an immortalized human prostate stromal cell line model.

实验应用 : ICC/IF

种属反应 : Human

Jung H et al. Mol Cell Biochem 2016; 423 (43102):21-28 Cytokeratin 18 is necessary for initiation of TGF-β1-induced epithelial-mesenchymal transition in breast epithelial cells.

实验应用 : WB

种属反应 : Human

Rakhila H et al. J Clin Endocrinol Metab 2016;:jc20161569 Augmented angiogenic factors expression via FP signaling pathways in peritoneal endometriosis.

实验应用 : ICC/IF

种属反应 : Human

SDS
PBS.pdf

产品包装	报价 (¥)
100 μl	¥ 4000
25 μl	¥ 1700
Qty 客制化偶联服务

	(全国热线) 4006-800-892 / (深圳) 0755-26755892 / (北京) 010-88594029 / (上海) 021-34613729 / (广州) 18024516375 / (香港) +852-69410778
	http://www.neobioscience.com
	genetex@neobioscience.com

Vimentin抗体 [GT812]

目录号 GTX629744

宿主

克隆

克隆编号

同种型

实验应用

种属反应

产品应用

应用说明

预期分子量

阳性对照

产品说明

预测种属反应

产品属性

形式

存储溶液

存放说明

浓度

免疫原种属

免疫原

纯化方式

偶联

RRID

注意事项

相关讯息

别名

功能与背景

数据库

研究领域

產品图片

GTX629744 IHC-Fr Image

GTX629744 WB Image

GTX629744 WB Image

GTX629744 WB Image

GTX629744 WB Image

GTX629744 IHC-P Image

GTX629744 WB Image

GTX629744 ELISA Image

GTX629744 WB Image

GTX629744 WB Image

GTX629744 IHC-Fr Image

GTX629744 ELISA Image

GTX629744 WB Image

GTX629744 WB Image

文献引用

Application Reference

产品评论

Vimentin antibody [GT812] 目录号 GTX629744

产品

研究

支持

公司

Follow Us

粤ICP备08007346号-9

© 1998-2022 GeneTex, Inc. 保留所有权利。

所有产品仅供研究使用 - 不应用于诊断或治疗。