Wild-type (WT) and Vimentin knockout (KO) HeLa cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with Vimentin antibody [HL1506] (GTX636980) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Various whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membranes were blotted with Vimentin antibody [HL1506] (GTX636980) diluted at 1:5000 and competitor's antibody (# Highly Cited Antibody) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody. *The competitor is not affiliated with GeneTex and does not endorse this product.

Vimentin antibody [HL1506] detects Vimentin protein at cytoplasm by immunohistochemical analysis.
Sample: Paraffin-embedded cat lymph node.
Vimentin stained by Vimentin antibody [HL1506] (GTX636980) diluted at 1:100.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Vimentin antibody [HL1506] detects Vimentin protein by immunohistochemical analysis.
Sample: Paraffin-embedded mouse tissue.
Vimentin stained by Vimentin antibody [HL1506] (GTX636980) diluted at 1:100.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Vimentin antibody [HL1506] detects Vimentin protein by immunohistochemical analysis.
Sample: Paraffin-embedded rat tissue.
Vimentin stained by Vimentin antibody [HL1506] (GTX636980) diluted at 1:100.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Vimentin antibody [HL1506] detects Vimentin protein at cytoskeleton and cell junction by immunofluorescent analysis.
Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: Vimentin stained by Vimentin antibody [HL1506] (GTX636980) diluted at 1:500.
Red: alpha Tubulin, a cytoskeleton marker, stained by alpha Tubulin antibody [GT114] (GTX628802) diluted at 1:1000.
Blue: Fluoroshield with DAPI (GTX30920).

Non-transfected (–) and transfected (+) 293T whole cell extracts (10 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with Vimentin antibody [HL1506] (GTX636980) diluted at 1:25000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Vimentin antibody [HL1506] detects Vimentin protein at cell membrane and cytoplasm by immunohistochemical analysis.
Sample: Paraffin-embedded human lung cancer.
Vimentin stained by Vimentin antibody [HL1506] (GTX636980) diluted at 1:100.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Vimentin antibody [HL1506] detects Vimentin protein by immunohistochemical analysis.
Sample: Paraffin-embedded mouse eye.
Green: Vimentin stained by Vimentin antibody [HL1506] (GTX636980) diluted at 1:100.
Red: beta Tubulin 3/ Tuj1, a Cytoskeleton marker, stained by beta Tubulin 3/ Tuj1 antibody [GT11710] (GTX631836) diluted at 1:500.
Blue: Fluoroshield with DAPI (GTX30920).
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Various whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with Vimentin antibody [HL1506] (GTX636980) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.

Various whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with Vimentin antibody [HL1506] (GTX636980) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Vimentin antibody [HL1506] detects Vimentin protein at cytoplasm by immunohistochemical analysis.
Sample: Paraffin-embedded human breast carcinoma.
Vimentin stained by Vimentin antibody [HL1506] (GTX636980) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min