产品应用
应用说明
*最佳稀释倍数与浓度应由研究人员确认
| 实验应用 |
稀释倍数 |
| 1:500-1:3000 |
| Assay dependent |
WB: 免疫印迹
ICC/IF: 细胞染色
IHC-P: 石蜡切片
IHC-Fr: 冰冻切片
预期分子量
预测种属反应
Bovine, Chicken, Pig, Xenopus tropicalis(>80% identity)
产品属性
形式
Liquid
存储溶液
PBS
存放说明
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
浓度
0.5 mg/ml (Please refer to the vial label for the specific concentration.)
免疫原种属
Human
免疫原
Recombinant protein encompassing a sequence within the N-terminus region of human Vinculin. The exact sequence is proprietary.
纯化方式
Affinity purified by Protein A.
偶联
Unconjugated
注意事项
仅供实验室使用。不适用于人类或动物的任何临床，治疗或诊断用途。不适合动物或人类食用。
相关讯息
别名
vinculin , CMD1W , CMH15 , HEL114 , MV , MVCL
细胞定位
Cell membrane,Cytoplasm, cytoskeleton
功能与背景
Vinculin is a cytoskeletal protein associated with cell-cell and cell-matrix junctions, where it is thought to function as one of several interacting proteins involved in anchoring F-actin to the membrane. Defects in VCL are the cause of cardiomyopathy dilated type 1W. Dilated cardiomyopathy is a disorder characterized by ventricular dilation and impaired systolic function, resulting in congestive heart failure and arrhythmia. Multiple alternatively spliced transcript variants have been found for this gene, but the biological validity of some variants has not been determined. [provided by RefSeq, Jul 2008]
数据库
研究领域
產品图片
![Anti-Vinculin antibody [HL1873] used in Western Blot (WB). GTX637622](/templates/default/js/lightbox/images/loading.gif "Anti-Vinculin antibody [HL1873] used in Western Blot (WB). GTX637622")
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GTX637622 WB Image
Various whole cell extracts (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with Vinculin antibody [HL1873] (GTX637622) diluted at 1:3000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody. Corresponding RNA expression data for the same cell lines are based on Human Protein Atlas program.
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GTX637622 IHC-P Image
Vinculin antibody [HL1873] detects Vinculin protein at cytoplasm by immunohistochemical analysis.Sample: Paraffin-embedded mouse stomach.Vinculin stained by Vinculin antibody [HL1873] (GTX637622) diluted at 1:100.Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
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GTX637622 WB Image
Whole zebrafish extract (30 μg) was separated by 5% SDS-PAGE, and the membrane was blotted with Vinculin antibody [HL1873] (GTX637622) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
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GTX637622 WB Image
Various whole cell extracts (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with Vinculin antibody [HL1873] (GTX637622) diluted at 1:3000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
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GTX637622 WB Image
Various whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with Vinculin antibody [HL1873] (GTX637622) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
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GTX637622 WB Image
Various whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with Vinculin antibody [HL1873] (GTX637622) diluted at 1:3000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
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文献引用
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产品评论
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![Anti-Vinculin antibody [HL1873] used in Western Blot (WB). GTX637622](/upload/website/prouct_img/small/GTX637622/GTX637622_44879_20221202_WB_TPM_watermark_22120719_521.webp "Anti-Vinculin antibody [HL1873] used in Western Blot (WB). GTX637622")
![Anti-Vinculin antibody [HL1873] used in IHC (Paraffin sections) (IHC-P). GTX637622](/upload/website/prouct_img/small/GTX637622/GTX637622_T-44830_20221028_IHC-P_M_22122018_318.webp "Anti-Vinculin antibody [HL1873] used in IHC (Paraffin sections) (IHC-P). GTX637622")
![Anti-Vinculin antibody [HL1873] used in Western Blot (WB). GTX637622](/upload/website/prouct_img/small/GTX637622/GTX637622_T-44830_20221118_WB_Z_22112219_412.webp "Anti-Vinculin antibody [HL1873] used in Western Blot (WB). GTX637622")
![Anti-Vinculin antibody [HL1873] used in Western Blot (WB). GTX637622](/upload/website/prouct_img/small/GTX637622/GTX637622_T-44830_20221118_WB_D_C_22112219_629.webp "Anti-Vinculin antibody [HL1873] used in Western Blot (WB). GTX637622")
![Anti-Vinculin antibody [HL1873] used in Western Blot (WB). GTX637622](/upload/website/prouct_img/small/GTX637622/GTX637622_T-44830_20221021_WB_M_R_22102723_944.webp "Anti-Vinculin antibody [HL1873] used in Western Blot (WB). GTX637622")
![Anti-Vinculin antibody [HL1873] used in Western Blot (WB). GTX637622](/upload/website/prouct_img/small/GTX637622/GTX637622_T-44830_20221104_WB_22110919_315.webp "Anti-Vinculin antibody [HL1873] used in Western Blot (WB). GTX637622")
![Anti-Vinculin antibody [HL1873] used in Western Blot (WB). GTX637622](/upload/website/prouct_img/normal/GTX637622/GTX637622_44879_20221202_WB_TPM_watermark_22120719_521.webp "Anti-Vinculin antibody [HL1873] used in Western Blot (WB). GTX637622")
![Anti-Vinculin antibody [HL1873] used in IHC (Paraffin sections) (IHC-P). GTX637622](/upload/website/prouct_img/normal/GTX637622/GTX637622_T-44830_20221028_IHC-P_M_22122018_318.webp "Anti-Vinculin antibody [HL1873] used in IHC (Paraffin sections) (IHC-P). GTX637622")
![Anti-Vinculin antibody [HL1873] used in Western Blot (WB). GTX637622](/upload/website/prouct_img/normal/GTX637622/GTX637622_T-44830_20221118_WB_Z_22112219_412.webp "Anti-Vinculin antibody [HL1873] used in Western Blot (WB). GTX637622")
![Anti-Vinculin antibody [HL1873] used in Western Blot (WB). GTX637622](/upload/website/prouct_img/normal/GTX637622/GTX637622_T-44830_20221118_WB_D_C_22112219_629.webp "Anti-Vinculin antibody [HL1873] used in Western Blot (WB). GTX637622")
![Anti-Vinculin antibody [HL1873] used in Western Blot (WB). GTX637622](/upload/website/prouct_img/normal/GTX637622/GTX637622_T-44830_20221021_WB_M_R_22102723_944.webp "Anti-Vinculin antibody [HL1873] used in Western Blot (WB). GTX637622")
![Anti-Vinculin antibody [HL1873] used in Western Blot (WB). GTX637622](/upload/website/prouct_img/normal/GTX637622/GTX637622_T-44830_20221104_WB_22110919_315.webp "Anti-Vinculin antibody [HL1873] used in Western Blot (WB). GTX637622")
