GeneTex中国官方网站
  • China

Vinculin抗体 [HL1964]

Recombinant antibody Orthogonal
Anti-Vinculin antibody [HL1964] used in Western Blot (WB). GTX637857
Anti-Vinculin antibody [HL1964] used in Western Blot (WB). GTX637857
Anti-Vinculin antibody [HL1964] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX637857
Anti-Vinculin antibody [HL1964] used in IHC (Paraffin sections) (IHC-P). GTX637857
Anti-Vinculin antibody [HL1964] used in Western Blot (WB). GTX637857
Anti-Vinculin antibody [HL1964] used in Western Blot (WB). GTX637857
Anti-Vinculin antibody [HL1964] used in Western Blot (WB). GTX637857

目录号 GTX637857

文献引用 1

宿主

 Rabbit

克隆

 Monoclonal

克隆编号

 HL1964

同种型

 IgG

实验应用

 WB, ICC/IF, IHC-P

种属反应

 Human, Mouse, Rat, Cat, Dog
产品包装
100 μl (¥4000),
25 μl (¥1700)
查看所有 Vinculin 的产品

产品应用

应用说明

*最佳稀释倍数与浓度应由研究人员确认
实验应用 稀释倍数
WB 1:500-1:3000
ICC/IF Assay dependent
IHC-P Assay dependent
WB: 免疫印迹
ICC/IF: 细胞染色
IHC-P: 石蜡切片
IHC-Fr: 冰冻切片

预期分子量

124 kDa. ( Note )

预测种属反应

Bovine, Chicken, Pig, Xenopus tropicalis(>80% identity)

产品属性

形式

Liquid

存储溶液

PBS

存放说明

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

浓度

1 mg/ml (Please refer to the vial label for the specific concentration.)

免疫原种属

Human

免疫原

Recombinant fragment of human Vinculin

纯化方式

Affinity purified by Protein A.

偶联

Unconjugated

注意事项

仅供实验室使用。不适用于人类或动物的任何临床，治疗或诊断用途。不适合动物或人类食用。

相关讯息

别名

vinculin , CMD1W , CMH15 , HEL114 , MV , MVCL

细胞定位

Cell membrane,Cytoplasm, cytoskeleton

功能与背景

Vinculin is a cytoskeletal protein associated with cell-cell and cell-matrix junctions, where it is thought to function as one of several interacting proteins involved in anchoring F-actin to the membrane. Defects in VCL are the cause of cardiomyopathy dilated type 1W. Dilated cardiomyopathy is a disorder characterized by ventricular dilation and impaired systolic function, resulting in congestive heart failure and arrhythmia. Multiple alternatively spliced transcript variants have been found for this gene, but the biological validity of some variants has not been determined. [provided by RefSeq, Jul 2008]

数据库

研究领域

產品图片

Anti-Vinculin antibody [HL1964] used in Western Blot (WB). GTX637857

GTX637857 WB Image

Various whole cell extracts (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with Vinculin antibody [HL1964] (GTX637857) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-Vinculin antibody [HL1964] used in Western Blot (WB). GTX637857

GTX637857 WB Image

Orthogonal

Various whole cell extracts (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with Vinculin antibody [HL1964] (GTX637857) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody. Corresponding RNA expression data for the same cell lines are based on Human Protein Atlas program.

Anti-Vinculin antibody [HL1964] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX637857

GTX637857 ICC/IF Image

Vinculin antibody [HL1964] detects Vinculin protein at cell junction by immunofluorescent analysis.
Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: Vinculin stained by Vinculin antibody [HL1964] (GTX637857) diluted at 1:500.
Blue: Fluoroshield with DAPI (GTX30920).

Anti-Vinculin antibody [HL1964] used in IHC (Paraffin sections) (IHC-P). GTX637857

GTX637857 IHC-P Image

Vinculin antibody [HL1964] detects Vinculin protein at cell membrane and cytoplasm by immunohistochemical analysis.
Sample: Paraffin-embedded rat duodenum.
Vinculin stained by Vinculin antibody [HL1964] (GTX637857) diluted at 1:100.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-Vinculin antibody [HL1964] used in Western Blot (WB). GTX637857

GTX637857 WB Image

Whole cell extract (30 μg) was separated by 5% SDS-PAGE, and the membrane was blotted with Vinculin antibody [HL1964] (GTX637857) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-Vinculin antibody [HL1964] used in Western Blot (WB). GTX637857

GTX637857 WB Image

Various whole cell extracts (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with Vinculin antibody [HL1964] (GTX637857) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-Vinculin antibody [HL1964] used in Western Blot (WB). GTX637857

GTX637857 WB Image

Whole cell extract (30 μg) was separated by 5% SDS-PAGE, and the membrane was blotted with Vinculin antibody [HL1964] (GTX637857) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

文献引用

Application Reference

Chinellato M et al. Pharmaceuticals (Basel) 2023; 16 (10) 1-Piperidine Propionic Acid as an Allosteric Inhibitor of Protease Activated Receptor-2.
我要回报文献

产品评论

目前没有使用 Vinculin antibody [HL1964] (GTX637857) 的产品评论被收录。快发表第一篇产品评论吧！
我要评论产品
产品包装 报价 (¥)
¥ 4000
¥ 1700
客制化偶联服务
联系经销商
欣博盛生物科技有限公司
欣博盛生物科技有限公司
(全国热线) 4006-800-892 / (深圳) 0755-26755892 / (北京) 010-88594029 / (上海) 021-34613729 / (广州) 18024516375 / (香港) +852-69410778
http://www.neobioscience.com
genetex@neobioscience.com