Vinculin抗体 [N3C1], Internal

Name: Vinculin抗体 [N3C1], Internal
Brand: GeneTex
SKU: GTX109749
Price: 4000.00 RMB
Rating: 5 (2 reviews)

Anti-Vinculin antibody [N3C1], Internal used in Western Blot (WB). GTX109749

Anti-Vinculin antibody [N3C1], Internal used in IHC (Paraffin sections) (IHC-P). GTX109749

目录号 GTX109749

文献引用 12

宿主	Rabbit
克隆	Polyclonal
同种型	IgG
实验应用	WB, IHC-P
种属反应	Human, Mouse, Rat, Pig
产品包装	100 μl (¥4000), 25 μl (¥1700)

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View product citations for antibody GTX109749 on CiteAb

产品应用

应用说明

*最佳稀释倍数与浓度应由研究人员确认

实验应用	稀释倍数
WB	1:500-1:3000
IHC-P	1:100-1:1000

WB: 免疫印迹
ICC/IF: 细胞染色
IHC-P: 石蜡切片
IHC-Fr: 冰冻切片

预期分子量

124 kDa. ( Note )

阳性对照

PC-12 , Rat2 , Mouse muscle

预测种属反应

Bovine, Chicken, Xenopus tropicalis(>80% identity)

产品属性

形式

Liquid

存储溶液

PBS, 1% BSA, 20% Glycerol

存放说明

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

浓度

0.11 mg/ml (Please refer to the vial label for the specific concentration.)

免疫原种属

Human

免疫原

Recombinant protein encompassing a sequence within the center region of human Vinculin. The exact sequence is proprietary.

纯化方式

Purified by antigen-affinity chromatography.

偶联

Unconjugated

RRID

AB_1952540

注意事项

仅供实验室使用。不适用于人类或动物的任何临床，治疗或诊断用途。不适合动物或人类食用。

别名

vinculin , CMD1W , CMH15 , HEL114 , MV , MVCL

细胞定位

Cytoplasm , cytoskeleton , Cell junction , adherens junction , Cell membrane; Peripheral membrane protein; Cytoplasmic side

功能与背景

Vinculin is a cytoskeletal protein associated with cell-cell and cell-matrix junctions, where it is thought to function as one of several interacting proteins involved in anchoring F-actin to the membrane. Defects in VCL are the cause of cardiomyopathy dilated type 1W. Dilated cardiomyopathy is a disorder characterized by ventricular dilation and impaired systolic function, resulting in congestive heart failure and arrhythmia. Multiple alternatively spliced transcript variants have been found for this gene, but the biological validity of some variants has not been determined. [provided by RefSeq]

数据库

Gene ID: 7414 VCL
UniProt: P18206 VCL

研究领域

產品图片

GTX109749 WB Image

Vinculin antibody [N3C1], Internal detects VCL protein by Western blot analysis.
A. 30 μg PC-12 whole cell lysate/extract
B. 30 μg Rat2 whole cell lysate/extract
5 % SDS-PAGE
Vinculin antibody [N3C1], Internal (GTX109749) dilution: 1:1000

GTX109749 IHC-P Image

Immunohistochemical analysis of human breast cancerncer, using Vinculin(GTX109749) antibody at 1:100 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

GTX109749 WB Image

Various whole cell extracts (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with Vinculin antibody [N3C1], Internal (GTX109749) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

GTX109749 WB Image

Mouse tissue extract (50 μg) was separated by 5% SDS-PAGE, and the membrane was blotted with Vinculin antibody [N3C1], Internal (GTX109749) diluted at 1:1000.

GTX109749 WB Image

The data was published in the journal Sci Rep in 2019.PMID: 31892709

GTX109749 WB Image

The data was published in the 2022 in Pharmaceuticals (Basel). PMID: 35215353

GTX109749 WB Image

The data was published in the 2022 in Pharmaceuticals (Basel). PMID: 35215353

GTX109749 WB Image

The data was published in the 2022 in Pharmaceuticals (Basel). PMID: 35215353

文献引用

Application Reference

Lee YZ et al. Pharmaceutics 2022; 14 (7) The Synergistic Inhibition of Coronavirus Replication and Induced Cytokine Production by Ciclesonide and the Tylophorine-Based Compound Dbq33b.

Yang CW et al. Pharmaceuticals (Basel) 2022; 15 (2) Tyrphostin AG1024 Suppresses Coronaviral Replication by Downregulating JAK1 via an IR/IGF-1R Independent Proteolysis Mediated by Ndfip1/2_NEDD4-like E3 Ligase Itch.

实验应用 : WB

种属反应 : Human

James S Novak et al. J Neuromuscul Dis 2021; 8 (s2) : S383-S402 Interrogation of Dystrophin and Dystroglycan Complex Protein Turnover After Exon Skipping Therapy.

实验应用 : WB

种属反应 : Mouse

Shannon Cunningham et al. Cancers (Basel) 2021; 13 (5) FLASH Proton Pencil Beam Scanning Irradiation Minimizes Radiation-Induced Leg Contracture and Skin Toxicity in Mice.

实验应用 : WB

种属反应 : Mouse

Cunningham S. et al. Cancers 2021; 13 (5) : 1012 FLASH Proton Pencil Beam Scanning Irradiation Minimizes Radiation-Induced Leg Contracture and Skin Toxicity in Mice.

实验应用 : WB

种属反应 : Mouse

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我要回报文献

产品评论

Vinculin antibody [N3C1], Internal 目录号 GTX109749

Rating

( Average 5 based on 2 users reviews)

实验应用

Western Blot(WB)

( Average 5 based on 2 users reviews)

Date : Anonymous submitted on 16-Jun-2020

Score :

Application Tested : WB

Sample Species : Ms

Sample : Raw264.7 cells

Amount used : 20 μg

Blocking : 3% BSA in TBST, 25°C, 1Hr

Primary Antibody : 1:1000, 4°C, 16Hr

Date : Anonymous submitted on 29-Oct-2019

Score :

Application Tested : WB

Sample Species : Hu

Sample : MO3.13 cells

Amount used : 10 μg

Blocking : Horse serum, 25°C, 1Hr

Primary Antibody : 1:10000, 4°C, 16Hr

我要评论产品

Application Reference

Lee YZ et al. Pharmaceutics 2022; 14 (7) The Synergistic Inhibition of Coronavirus Replication and Induced Cytokine Production by Ciclesonide and the Tylophorine-Based Compound Dbq33b.

实验应用 : WB

种属反应 : Human

James S Novak et al. J Neuromuscul Dis 2021; 8 (s2):S383-S402 Interrogation of Dystrophin and Dystroglycan Complex Protein Turnover After Exon Skipping Therapy.

实验应用 : WB

种属反应 : Mouse

Shannon Cunningham et al. Cancers (Basel) 2021; 13 (5) FLASH Proton Pencil Beam Scanning Irradiation Minimizes Radiation-Induced Leg Contracture and Skin Toxicity in Mice.

实验应用 : WB

种属反应 : Mouse

Cunningham S. et al. Cancers 2021; 13 (5):1012 FLASH Proton Pencil Beam Scanning Irradiation Minimizes Radiation-Induced Leg Contracture and Skin Toxicity in Mice.

实验应用 : WB

种属反应 : Mouse

Yang CW et al. Biochem Pharmacol 2020; 180:114122 Natural cardenolides suppress coronaviral replication by downregulating JAK1 via a Na⁺/K⁺-ATPase independent proteolysis.

Macchi C et al. Am J Pathol 2020; 190 (11):2226-2236 Leptin, Resistin, and Proprotein Convertase Subtilisin/Kexin Type 9: The Role of STAT3.

实验应用 : WB

种属反应 : Human

Huang HY et al. J Cell Mol Med 2020; Progesterone receptor membrane component 1 is involved in oral cancer cell metastasis.

实验应用 : WB

种属反应 : Human

Chou CW et al. Sci Rep 2019; 9 (1):20403 Therapeutic effects of statins against lung adenocarcinoma via p53 mutant-mediated apoptosis.

Lee YZ et al. Pharmacol Res 2019; 152:104581 Tylophorine-based compounds are therapeutic in rheumatoid arthritis by targeting the caprin-1 ribonucleoprotein complex and inhibiting expression of associated c-Myc and HIF-1帢.

实验应用 : WB

种属反应 : Rat

Panneerselvam L et al. Biotechnol Appl Biochem 2019; Ferulic acid attenuates arsenic induced cardiotoxicity in rats.

实验应用 : WB

Brandi J et al. J Proteomics 2016; 136:43112 Secretome protein signature of human pancreatic cancer stem-like cells.

实验应用 : WB

种属反应 : Human

SDS
PBS.pdf
Glycerol.pdf
Proclin.pdf

产品包装	报价 (¥)
100 μl	¥ 4000
25 μl	¥ 1700
Qty

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	http://www.neobioscience.com
	genetex@neobioscience.com

Vinculin抗体 [N3C1], Internal

目录号 GTX109749

宿主

克隆

同种型

实验应用

种属反应

产品应用

应用说明

预期分子量

阳性对照

预测种属反应

产品属性

形式

存储溶液

存放说明

浓度

免疫原种属

免疫原

纯化方式

偶联

RRID

注意事项

相关讯息

别名

细胞定位

功能与背景

数据库

研究领域

產品图片

GTX109749 WB Image

GTX109749 IHC-P Image

GTX109749 WB Image

GTX109749 WB Image

GTX109749 WB Image

GTX109749 WB Image

GTX109749 WB Image

GTX109749 WB Image

GTX109749 WB Image

文献引用

Application Reference

产品评论

Vinculin antibody [N3C1], Internal 目录号 GTX109749

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