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Von Hippel Lindau抗体

Overexpression
Anti-Von Hippel Lindau antibody used in IHC (Paraffin sections) (IHC-P). GTX101087
Anti-Von Hippel Lindau antibody used in IHC (Paraffin sections) (IHC-P). GTX101087
Anti-Von Hippel Lindau antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX101087
Anti-Von Hippel Lindau antibody used in Western Blot (WB). GTX101087
Anti-Von Hippel Lindau antibody used in Western Blot (WB). GTX101087
Anti-Von Hippel Lindau antibody used in IHC (Paraffin sections) (IHC-P). GTX101087

目录号 GTX101087

文献引用 13

宿主

 Rabbit

克隆

 Polyclonal

同种型

 IgG

实验应用

 WB, ICC/IF, IHC-P, IP

种属反应

 Human, Mouse, Rat
产品包装
100 μl (¥4000),
25 μl (¥1700)
查看所有 Von Hippel Lindau 的产品

产品应用

应用说明

*最佳稀释倍数与浓度应由研究人员确认
实验应用 稀释倍数
WB 1:500-1:10000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
IP Assay dependent
WB: 免疫印迹
ICC/IF: 细胞染色
IHC-P: 石蜡切片
IHC-Fr: 冰冻切片

预期分子量

24 kDa. ( Note )

阳性对照

Myc-DDK-tagged VHL-transfected 293T

预测种属反应

Bovine, Dog(>80% identity)

产品属性

形式

Liquid

存储溶液

PBS, 20% Glycerol

存放说明

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

浓度

1.27 mg/ml (Please refer to the vial label for the specific concentration.)

免疫原种属

Human

免疫原

Recombinant protein encompassing a sequence within the center region of human Von Hippel Lindau. The exact sequence is proprietary.

纯化方式

Purified by antigen-affinity chromatography.

偶联

Unconjugated

RRID

AB_1952549

注意事项

仅供实验室使用。不适用于人类或动物的任何临床，治疗或诊断用途。不适合动物或人类食用。

相关讯息

别名

von Hippel-Lindau tumor suppressor , HRCA1 , RCA1 , VHL1 , pVHL

细胞定位

Isoform 1: Cytoplasm , Membrane; Peripheral membrane protein , Nucleus , Isoform 3: Cytoplasm

功能与背景

Von Hippel-Lindau syndrome (VHL) is a dominantly inherited familial cancer syndrome predisposing to a variety of malignant and benign tumors. A germline mutation of this gene is the basis of familial inheritance of VHL syndrome. The protein encoded by this gene is a component of the protein complex that includes elongin B, elongin C, and cullin-2, and possesses ubiquitin ligase E3 activity. This protein is involved in the ubiquitination and degradation of hypoxia-inducible-factor (HIF), which is a transcription factor that plays a central role in the regulation of gene expression by oxygen. RNA polymerase II subunit POLR2G/RPB7 is also reported to be a target of this protein. Alternatively spliced transcript variants encoding distinct isoforms have been observed. [provided by RefSeq]

数据库

研究领域

產品图片

Anti-Von Hippel Lindau antibody used in IHC (Paraffin sections) (IHC-P). GTX101087

GTX101087 IHC-P Image

Von Hippel Lindau antibody detects Von Hippel Lindau protein at cytoplasm by immunohistochemical analysis.
Sample: Paraffin-embedded rat intestine.
Von Hippel Lindau stained by Von Hippel Lindau antibody (GTX101087) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-Von Hippel Lindau antibody used in IHC (Paraffin sections) (IHC-P). GTX101087

GTX101087 IHC-P Image

Von Hippel Lindau antibody detects Von Hippel Lindau protein at cytoplasm by immunohistochemical analysis.
Sample: Paraffin-embedded mouse kidney.
Von Hippel Lindau stained by Von Hippel Lindau antibody (GTX101087) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-Von Hippel Lindau antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX101087

GTX101087 ICC/IF Image

Von Hippel Lindau antibody detects Von Hippel Lindau protein at cytoplasm and nucleus by immunofluorescent analysis.
Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: Von Hippel Lindau stained by Von Hippel Lindau antibody (GTX101087) diluted at 1:500.
Red: alpha Tubulin, a cytoskeleton marker, stained by alpha Tubulin antibody [GT114] (GTX628802) diluted at 1:1000.
Blue: Fluoroshield with DAPI (GTX30920).
Scale bar= 10μm.

Anti-Von Hippel Lindau antibody used in Western Blot (WB). GTX101087

GTX101087 WB Image

Overexpression

Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with Von Hippel Lindau antibody (GTX101087) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-Von Hippel Lindau antibody used in Western Blot (WB). GTX101087

GTX101087 WB Image

Various whole cell extracts (30 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with Von Hippel Lindau antibody (GTX101087) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-Von Hippel Lindau antibody used in IHC (Paraffin sections) (IHC-P). GTX101087

GTX101087 IHC-P Image

Von Hippel Lindau antibody detects Von Hippel Lindau protein at nucleus by immunohistochemical analysis.
Sample: Paraffin-embedded mouse lung.
Von Hippel Lindau stained by Von Hippel Lindau antibody (GTX101087) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

文献引用

Application Reference

Tang Y et al. Mol Brain 2023; 16 (1) : 74 MLKL regulates Cx43 ubiquitinational degradation and mediates neuronal necroptosis in ipsilateral thalamus after focal cortical infarction.
Yang KS et al. Sci Rep 2023; 13 (1) : 10969 Extracellular vesicle analysis of plasma allows differential diagnosis of atypical pancreatic serous cystadenoma.
Chittiboina P et al. Clin Cancer Res 2023; 29 (12) : 2199-2209 Proteostasis Modulation in Germline Missense von Hippel Lindau Disease.
Li G et al. Exp Mol Med 2022; 54 (7) : 988-998 Homocysteine-targeting compounds as a new treatment strategy for diabetic wounds via inhibition of the histone methyltransferase SET7/9.
Tianli Chen et al. Mol Ther 2021; 29 (6) : 2134-2150 WDR5 facilitates EMT and metastasis of CCA by increasing HIF-1α accumulation in Myc-dependent and independent pathways.
种属反应 : Human
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Application Reference
Tang Y et al. Mol Brain 2023; 16 (1):74 MLKL regulates Cx43 ubiquitinational degradation and mediates neuronal necroptosis in ipsilateral thalamus after focal cortical infarction.
Yang KS et al. Sci Rep 2023; 13 (1):10969 Extracellular vesicle analysis of plasma allows differential diagnosis of atypical pancreatic serous cystadenoma.
Chittiboina P et al. Clin Cancer Res 2023; 29 (12):2199-2209 Proteostasis Modulation in Germline Missense von Hippel Lindau Disease.
Li G et al. Exp Mol Med 2022; 54 (7):988-998 Homocysteine-targeting compounds as a new treatment strategy for diabetic wounds via inhibition of the histone methyltransferase SET7/9.
Tianli Chen et al. Mol Ther 2021; 29 (6):2134-2150 WDR5 facilitates EMT and metastasis of CCA by increasing HIF-1α accumulation in Myc-dependent and independent pathways.
种属反应 : Human
Shih HJ et al. Sci Rep 2021; 11 (1):22925 Differential expression of hypoxia-inducible factors related to the invasiveness of epithelial ovarian cancer.
实验应用 : WB, IHC-P
种属反应 : Human
Li G et al. Nat Commun 2021; 12 (1):3363 A small molecule HIF-1α stabilizer that accelerates diabetic wound healing.
实验应用 : WB
种属反应 : Human
Xu S et al. Cell Death Dis 2019; 10 (3):191 CXCR7 promotes melanoma tumorigenesis via Src kinase signaling.
实验应用 : WB
种属反应 : Human, Mouse
Lee SB et al. Nature 2016; 529 (7585):172-7 An ID2-dependent mechanism for VHL inactivation in cancer.
实验应用 : WB
种属反应 : Human
Dukel M et al. J Biol Chem 2016; The Breast Cancer Tumor Suppressor TRIM29 is Expressed via ATM-Dependent Signaling in Response to Hypoxia.
实验应用 : WB
种属反应 : Human
Li CH et al. Arch Toxicol 2014; 88 (3):659-71 Minocycline accelerates hypoxia-inducible factor-1 alpha degradation and inhibits hypoxia-induced neovasculogenesis through prolyl hydroxylase, von Hippel-Lindau-dependent pathway.
实验应用 : WB
种属反应 : Human
Yao P et al. PLoS Biol 2013; 11 (8):e1001635 The HILDA complex coordinates a conditional switch in the 3'-untranslated region of the VEGFA mRNA.
实验应用 : WB
种属反应 : Human
Lin CH et al. Br J Pharmacol 2013; 168 (4):920-31 Silibinin inhibits VEGF secretion and age-related macular degeneration in a hypoxia-dependent manner through the PI-3 kinase/Akt/mTOR pathway.
实验应用 : IP
种属反应 : Human
SDS
PBS.pdf
Glycerol.pdf
Proclin.pdf
产品包装 报价 (¥)
¥ 4000
¥ 1700
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