产品应用
应用说明
*最佳稀释倍数与浓度应由研究人员确认
| 实验应用 |
稀释倍数 |
| Assay dependent |
| Assay dependent |
| Assay dependent |
WB: 免疫印迹
ICC/IF: 细胞染色
IHC-P: 石蜡切片
IHC-Fr: 冰冻切片
预期分子量
产品属性
形式
Liquid
存储溶液
PBS
存放说明
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
浓度
2 mg/ml (Please refer to the vial label for the specific concentration.)
免疫原种属
Human
免疫原
Recombinant fragmemt of human WNK1
纯化方式
Affinity purified by Protein A.
偶联
Unconjugated
注意事项
仅供实验室使用。不适用于人类或动物的任何临床，治疗或诊断用途。不适合动物或人类食用。
相关讯息
别名
WNK lysine deficient protein kinase 1 , HSAN2 , HSN2 , KDP , PPP1R167 , PRKWNK1 , PSK , p65
细胞定位
Cytoplasm
功能与背景
This gene encodes a member of the WNK subfamily of serine/threonine protein kinases. The encoded protein may be a key regulator of blood pressure by controlling the transport of sodium and chloride ions. Mutations in this gene have been associated with pseudohypoaldosteronism type II and hereditary sensory neuropathy type II. Alternatively spliced transcript variants encoding different isoforms have been described but the full-length nature of all of them has yet to be determined.[provided by RefSeq, May 2010]
数据库
研究领域
產品图片
![Anti-WNK1 antibody [HL2184] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX638188](/templates/default/js/lightbox/images/loading.gif "Anti-WNK1 antibody [HL2184] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX638188")
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GTX638188 ICC/IF Image
WNK1 antibody [HL2184] detects WNK1 protein at cytoplasm by immunofluorescent analysis.Sample: DIV9 rat E18 primary cortical neuron cells were fixed in 4% paraformaldehyde at RT for 15 min.Green: WNK1 stained by WNK1 antibody [HL2184] (GTX638188) diluted at 1:250.Red: Tau, a axon marker, stained by Tau antibody [GT287] (GTX634809) diluted at 1:500.Blue: Fluoroshield with DAPI (GTX30920).
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GTX638188 IHC-P Image
WNK1 antibody [HL2184] detects WNK1 protein at cytoplasm by immunohistochemical analysis.Sample: Paraffin-embedded rat cerebellum.WNK1 stained by WNK1 antibody [HL2184] (GTX638188) diluted at 1:50.Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
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GTX638188 IHC-P Image
WNK1 antibody [HL2184] detects WNK1 protein at cytoplasm by immunohistochemical analysis.Sample: Paraffin-embedded mouse kidney.WNK1 stained by WNK1 antibody [HL2184] (GTX638188) diluted at 1:50.Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
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GTX638188 WB Image
Mouse tissue extract (50 μg) was separated by 5% SDS-PAGE, and the membrane was blotted with WNK1 antibody [HL2184] (GTX638188) diluted at 1:2000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
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GTX638188 ICC/IF Image
WNK1 antibody [HL2184] detects WNK1 protein at cytoplasm by immunofluorescent analysis.Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.Green: WNK1 stained by WNK1 antibody [HL2184] (GTX638188) diluted at 1:500.Blue: Fluoroshield with DAPI (GTX30920).
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GTX638188 WB Image
Various whole cell extracts (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with WNK1 antibody [HL2184] (GTX638188) diluted at 1:2000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody. Corresponding RNA expression data for the same cell lines are based on Human Protein Atlas program.
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文献引用
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产品评论
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![Anti-WNK1 antibody [HL2184] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX638188](/upload/website/prouct_img/small/GTX638188/GTX638188_T-44935_20230519_ICC_IF_R_23053001_704.webp "Anti-WNK1 antibody [HL2184] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX638188")
![Anti-WNK1 antibody [HL2184] used in IHC (Paraffin sections) (IHC-P). GTX638188](/upload/website/prouct_img/small/GTX638188/GTX638188_T-44935_20230217_IHC-P_R_23030219_792.webp "Anti-WNK1 antibody [HL2184] used in IHC (Paraffin sections) (IHC-P). GTX638188")
![Anti-WNK1 antibody [HL2184] used in IHC (Paraffin sections) (IHC-P). GTX638188](/upload/website/prouct_img/small/GTX638188/GTX638188_T-44935_20230217_IHC-P_M_23030219_803.webp "Anti-WNK1 antibody [HL2184] used in IHC (Paraffin sections) (IHC-P). GTX638188")
![Anti-WNK1 antibody [HL2184] used in Western Blot (WB). GTX638188](/upload/website/prouct_img/small/GTX638188/GTX638188_T-44935_20230303_WB_M_brain_23030717_445.webp "Anti-WNK1 antibody [HL2184] used in Western Blot (WB). GTX638188")
![Anti-WNK1 antibody [HL2184] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX638188](/upload/website/prouct_img/small/GTX638188/GTX638188_T-44935_20230317_ICC_IF_23041023_603.webp "Anti-WNK1 antibody [HL2184] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX638188")
![Anti-WNK1 antibody [HL2184] used in Western Blot (WB). GTX638188](/upload/website/prouct_img/small/GTX638188/GTX638188_45012_20230421_WB_TPM_watermark_23042500_918.webp "Anti-WNK1 antibody [HL2184] used in Western Blot (WB). GTX638188")
![Anti-WNK1 antibody [HL2184] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX638188](/upload/website/prouct_img/normal/GTX638188/GTX638188_T-44935_20230519_ICC_IF_R_23053001_704.webp "Anti-WNK1 antibody [HL2184] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX638188")
![Anti-WNK1 antibody [HL2184] used in IHC (Paraffin sections) (IHC-P). GTX638188](/upload/website/prouct_img/normal/GTX638188/GTX638188_T-44935_20230217_IHC-P_R_23030219_792.webp "Anti-WNK1 antibody [HL2184] used in IHC (Paraffin sections) (IHC-P). GTX638188")
![Anti-WNK1 antibody [HL2184] used in IHC (Paraffin sections) (IHC-P). GTX638188](/upload/website/prouct_img/normal/GTX638188/GTX638188_T-44935_20230217_IHC-P_M_23030219_803.webp "Anti-WNK1 antibody [HL2184] used in IHC (Paraffin sections) (IHC-P). GTX638188")
![Anti-WNK1 antibody [HL2184] used in Western Blot (WB). GTX638188](/upload/website/prouct_img/normal/GTX638188/GTX638188_T-44935_20230303_WB_M_brain_23030717_445.webp "Anti-WNK1 antibody [HL2184] used in Western Blot (WB). GTX638188")
![Anti-WNK1 antibody [HL2184] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX638188](/upload/website/prouct_img/normal/GTX638188/GTX638188_T-44935_20230317_ICC_IF_23041023_603.webp "Anti-WNK1 antibody [HL2184] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX638188")
![Anti-WNK1 antibody [HL2184] used in Western Blot (WB). GTX638188](/upload/website/prouct_img/normal/GTX638188/GTX638188_45012_20230421_WB_TPM_watermark_23042500_918.webp "Anti-WNK1 antibody [HL2184] used in Western Blot (WB). GTX638188")
