Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with Wnt16 antibody [HL1498] (GTX636972) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Wnt16 antibody [HL1498] detects secreted Wnt16 protein by immunohistochemical analysis.
Sample: Paraffin-embedded mouse kidney.
Wnt16 stained by Wnt16 antibody [HL1498] (GTX636972) diluted at 1:100.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Wnt16 antibody [HL1498] detects Wnt16 protein by immunofluorescent analysis.
Sample: Mock and transfected 293T cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: Wnt16 stained by Wnt16 antibody [HL1498] (GTX636972) diluted at 1:500.
Blue: Fluoroshield with DAPI (GTX30920).