产品应用
应用说明
*最佳稀释倍数与浓度应由研究人员确认
| 实验应用 |
稀释倍数 |
| 1:1000-1:10000 |
| Assay dependent |
| Assay dependent |
WB: 免疫印迹
ICC/IF: 细胞染色
IHC-P: 石蜡切片
IHC-Fr: 冰冻切片
预期分子量
产品说明
This antibody is specific for human Wnt3a protein, and it does not cross react with human Wnt1, Wnt2, Wnt2b, Wnt3, Wnt4, Wnt5a, Wnt6, Wnt7a, Wnt7b, Wnt8a, Wnt8b, Wnt9a, Wnt9b, Wnt10a, Wnt10b, and Wnt16 protein.
产品属性
形式
Liquid
存储溶液
PBS
存放说明
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
浓度
1 mg/ml (Please refer to the vial label for the specific concentration.)
免疫原种属
Human
免疫原
Recombinant fragment of human Wnt3a.
纯化方式
Affinity purified by Protein A.
偶联
Unconjugated
注意事项
仅供实验室使用。不适用于人类或动物的任何临床，治疗或诊断用途。不适合动物或人类食用。
相关讯息
别名
Wnt family member 3A
细胞定位
Secreted, extracellular space, extracellular matrix,Secreted
功能与背景
The WNT gene family consists of structurally related genes which encode secreted signaling proteins. These proteins have been implicated in oncogenesis and in several developmental processes, including regulation of cell fate and patterning during embryogenesis. This gene is a member of the WNT gene family. It encodes a protein which shows 96% amino acid identity to mouse Wnt3A protein, and 84% to human WNT3 protein, another WNT gene product. This gene is clustered with WNT14 gene, another family member, in chromosome 1q42 region. [provided by RefSeq, Jul 2008]
数据库
研究领域
產品图片
![Anti-Wnt3a antibody [HL1911] used in Western Blot (WB). GTX637660](/templates/default/js/lightbox/images/loading.gif "Anti-Wnt3a antibody [HL1911] used in Western Blot (WB). GTX637660")
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GTX637660 WB Image
Non-transfected (–) and transfected (+) 293T whole cell extracts were separated by 10% SDS-PAGE, and the membrane was blotted with Wnt3a antibody [HL1911] (GTX637660) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
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GTX637660 IHC-P Image
Wnt3a antibody [HL1911] detects secreted Wnt3a protein by immunohistochemical analysis.Sample: Paraffin-embedded human prostate cancer.Wnt3a stained by Wnt3a antibody [HL1911] (GTX637660) diluted at 1:100.Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
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GTX637660 IHC-P Image
Wnt3a antibody [HL1911] detects Wnt3a protein by immunohistochemical analysis.Sample: Paraffin-embedded rat tissues.Wnt3a stained by Wnt3a antibody [HL1911] (GTX637660) diluted at 1:200.Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
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GTX637660 ICC/IF Image
Wnt3a antibody [HL1911] detects Wnt3a protein by immunofluorescent analysis.Sample: Mock and transfected 293T cells were fixed in ice-cold MeOH for 5 min.Green: Wnt3a stained by Wnt3a antibody [HL1911] (GTX637660) diluted at 1:500.Blue: Fluoroshield with DAPI (GTX30920).Scale bar= 10μm.
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GTX637660 WB Image
Mouse tissue extract (50 μg) was separated by 10% SDS-PAGE, and the membrane was blotted with Wnt3a antibody [HL1911] (GTX637660) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
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GTX637660 WB Image
Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with Wnt3a antibody [HL1911] (GTX637660) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.
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文献引用
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产品评论
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![Anti-Wnt3a antibody [HL1911] used in Western Blot (WB). GTX637660](/upload/website/prouct_img/small/GTX637660/GTX637660_T-44837_20230616_WB_multiple_B_23062019_164.webp "Anti-Wnt3a antibody [HL1911] used in Western Blot (WB). GTX637660")
![Anti-Wnt3a antibody [HL1911] used in IHC (Paraffin sections) (IHC-P). GTX637660](/upload/website/prouct_img/small/GTX637660/GTX637660_45138_20230928_IHC-P_23103019_227.webp "Anti-Wnt3a antibody [HL1911] used in IHC (Paraffin sections) (IHC-P). GTX637660")
![Anti-Wnt3a antibody [HL1911] used in IHC (Paraffin sections) (IHC-P). GTX637660](/upload/website/prouct_img/small/GTX637660/GTX637660_45138_20231106_IHC-P_multiple_R_23111422_465.webp "Anti-Wnt3a antibody [HL1911] used in IHC (Paraffin sections) (IHC-P). GTX637660")
![Anti-Wnt3a antibody [HL1911] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX637660](/upload/website/prouct_img/small/GTX637660/GTX637660_45138_20231110_ICC_IF_B_2_23111422_688.webp "Anti-Wnt3a antibody [HL1911] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX637660")
![Anti-Wnt3a antibody [HL1911] used in Western Blot (WB). GTX637660](/upload/website/prouct_img/small/GTX637660/GTX637660_T-44837_20230804_WB_M_skin_23080901_299.webp "Anti-Wnt3a antibody [HL1911] used in Western Blot (WB). GTX637660")
![Anti-Wnt3a antibody [HL1911] used in Western Blot (WB). GTX637660](/upload/website/prouct_img/small/GTX637660/GTX637660_45138_20230825_WB_B_23083020_856.webp "Anti-Wnt3a antibody [HL1911] used in Western Blot (WB). GTX637660")
![Anti-Wnt3a antibody [HL1911] used in Western Blot (WB). GTX637660](/upload/website/prouct_img/normal/GTX637660/GTX637660_T-44837_20230616_WB_multiple_B_23062019_164.webp "Anti-Wnt3a antibody [HL1911] used in Western Blot (WB). GTX637660")
![Anti-Wnt3a antibody [HL1911] used in IHC (Paraffin sections) (IHC-P). GTX637660](/upload/website/prouct_img/normal/GTX637660/GTX637660_45138_20230928_IHC-P_23103019_227.webp "Anti-Wnt3a antibody [HL1911] used in IHC (Paraffin sections) (IHC-P). GTX637660")
![Anti-Wnt3a antibody [HL1911] used in IHC (Paraffin sections) (IHC-P). GTX637660](/upload/website/prouct_img/normal/GTX637660/GTX637660_45138_20231106_IHC-P_multiple_R_23111422_465.webp "Anti-Wnt3a antibody [HL1911] used in IHC (Paraffin sections) (IHC-P). GTX637660")
![Anti-Wnt3a antibody [HL1911] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX637660](/upload/website/prouct_img/normal/GTX637660/GTX637660_45138_20231110_ICC_IF_B_2_23111422_688.webp "Anti-Wnt3a antibody [HL1911] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX637660")
![Anti-Wnt3a antibody [HL1911] used in Western Blot (WB). GTX637660](/upload/website/prouct_img/normal/GTX637660/GTX637660_T-44837_20230804_WB_M_skin_23080901_299.webp "Anti-Wnt3a antibody [HL1911] used in Western Blot (WB). GTX637660")
![Anti-Wnt3a antibody [HL1911] used in Western Blot (WB). GTX637660](/upload/website/prouct_img/normal/GTX637660/GTX637660_45138_20230825_WB_B_23083020_856.webp "Anti-Wnt3a antibody [HL1911] used in Western Blot (WB). GTX637660")
