产品应用
应用说明
*最佳稀释倍数与浓度应由研究人员确认
| 实验应用 |
稀释倍数 |
| 1:1000-1:10000 |
| Assay dependent |
| Assay dependent |
WB: 免疫印迹
ICC/IF: 细胞染色
IHC-P: 石蜡切片
IHC-Fr: 冰冻切片
预期分子量
产品说明
This antibody is specific for human Wnt4 protein, and it does not cross react with human Wnt1, Wnt2, Wnt2b, Wnt3, Wnt3a, Wnt5a, Wnt6, Wnt7a, Wnt7b, Wnt8a, Wnt8b, Wnt9a, Wnt9b, Wnt10a, Wnt10b, and Wnt16 protein.
产品属性
形式
Liquid
存储溶液
PBS
存放说明
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
浓度
2 mg/ml (Please refer to the vial label for the specific concentration.)
免疫原种属
Human
免疫原
Recombinant fragment of human Wnt4.
纯化方式
Affinity purified by Protein A.
偶联
Unconjugated
注意事项
仅供实验室使用。不适用于人类或动物的任何临床，治疗或诊断用途。不适合动物或人类食用。
相关讯息
别名
Wnt family member 4 , SERKAL , WNT-4
细胞定位
Secreted, extracellular space, extracellular matrix
功能与背景
The WNT gene family consists of structurally related genes which encode secreted signaling proteins. These proteins have been implicated in oncogenesis and in several developmental processes, including regulation of cell fate and patterning during embryogenesis. This gene is a member of the WNT gene family, and is the first signaling molecule shown to influence the sex-determination cascade. It encodes a protein which shows 98% amino acid identity to the Wnt4 protein of mouse and rat. This gene and a nuclear receptor known to antagonize the testis-determining factor play a concerted role in both the control of female development and the prevention of testes formation. This gene and another two family members, WNT2 and WNT7B, may be associated with abnormal proliferation in breast tissue. Mutations in this gene can result in Rokitansky-Kuster-Hauser syndrome and in SERKAL syndrome. [provided by RefSeq, Jul 2008]
数据库
研究领域
產品图片
![Anti-Wnt4 antibody [HL1889] used in Western Blot (WB). GTX637638](/templates/default/js/lightbox/images/loading.gif "Anti-Wnt4 antibody [HL1889] used in Western Blot (WB). GTX637638")
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GTX637638 WB Image
Non-transfected (–) and transfected (+) 293T whole cell extracts were separated by 10% SDS-PAGE, and the membrane was blotted with Wnt4 antibody [HL1889] (GTX637638) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.
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GTX637638 ICC/IF Image
Wnt4 antibody [HL1889] detects Wnt4 protein at cytoplasm by immunofluorescent analysis.Sample: HeLa cells were fixed in ice-cold MeOH for 5 min.Green: Wnt4 stained by Wnt4 antibody [HL1889] (GTX637638) diluted at 1:100.Blue: Fluoroshield with DAPI (GTX30920).
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GTX637638 IHC-P Image
Wnt4 antibody [HL1889] detects Wnt4 protein at cell membrane and cytoplasm by immunohistochemical analysis.Sample: Paraffin-embedded mouse kidney.Wnt4 stained by Wnt4 antibody [HL1889] (GTX637638) diluted at 1:100.Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
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GTX637638 WB Image
Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with Wnt4 antibody [HL1889] (GTX637638) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
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GTX637638 WB Image
Whole zebrafish extract (30 μg) was separated by 10% SDS-PAGE, and the membrane was blotted with Wnt4 antibody [HL1889] (GTX637638) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.
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文献引用
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产品评论
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![Anti-Wnt4 antibody [HL1889] used in Western Blot (WB). GTX637638](/upload/website/prouct_img/small/GTX637638/GTX637638_T-44837_20230421_WB_multiple_B_23042500_721.webp "Anti-Wnt4 antibody [HL1889] used in Western Blot (WB). GTX637638")
![Anti-Wnt4 antibody [HL1889] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX637638](/upload/website/prouct_img/small/GTX637638/GTX637638_45082_20230908_ICC_IF_23091901_986.webp "Anti-Wnt4 antibody [HL1889] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX637638")
![Anti-Wnt4 antibody [HL1889] used in IHC (Paraffin sections) (IHC-P). GTX637638](/upload/website/prouct_img/small/GTX637638/GTX637638_T-44837_20230811_IHC-P_M_23082201_204.webp "Anti-Wnt4 antibody [HL1889] used in IHC (Paraffin sections) (IHC-P). GTX637638")
![Anti-Wnt4 antibody [HL1889] used in Western Blot (WB). GTX637638](/upload/website/prouct_img/small/GTX637638/GTX637638_45082_20230630_WB_B_23062718_134.webp "Anti-Wnt4 antibody [HL1889] used in Western Blot (WB). GTX637638")
![Anti-Wnt4 antibody [HL1889] used in Western Blot (WB). GTX637638](/upload/website/prouct_img/small/GTX637638/GTX637638_45082_20230630_WB_Z_23062718_750.webp "Anti-Wnt4 antibody [HL1889] used in Western Blot (WB). GTX637638")
![Anti-Wnt4 antibody [HL1889] used in Western Blot (WB). GTX637638](/upload/website/prouct_img/normal/GTX637638/GTX637638_T-44837_20230421_WB_multiple_B_23042500_721.webp "Anti-Wnt4 antibody [HL1889] used in Western Blot (WB). GTX637638")
![Anti-Wnt4 antibody [HL1889] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX637638](/upload/website/prouct_img/normal/GTX637638/GTX637638_45082_20230908_ICC_IF_23091901_986.webp "Anti-Wnt4 antibody [HL1889] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX637638")
![Anti-Wnt4 antibody [HL1889] used in IHC (Paraffin sections) (IHC-P). GTX637638](/upload/website/prouct_img/normal/GTX637638/GTX637638_T-44837_20230811_IHC-P_M_23082201_204.webp "Anti-Wnt4 antibody [HL1889] used in IHC (Paraffin sections) (IHC-P). GTX637638")
![Anti-Wnt4 antibody [HL1889] used in Western Blot (WB). GTX637638](/upload/website/prouct_img/normal/GTX637638/GTX637638_45082_20230630_WB_B_23062718_134.webp "Anti-Wnt4 antibody [HL1889] used in Western Blot (WB). GTX637638")
![Anti-Wnt4 antibody [HL1889] used in Western Blot (WB). GTX637638](/upload/website/prouct_img/normal/GTX637638/GTX637638_45082_20230630_WB_Z_23062718_750.webp "Anti-Wnt4 antibody [HL1889] used in Western Blot (WB). GTX637638")
