产品应用
应用说明
*最佳稀释倍数与浓度应由研究人员确认
| 实验应用 |
稀释倍数 |
| 1:1000-1:10000 |
| Assay dependent |
| Assay dependent |
WB: 免疫印迹
ICC/IF: 细胞染色
IHC-P: 石蜡切片
IHC-Fr: 冰冻切片
预期分子量
产品说明
This antibody is specific for human Wnt6 protein, and it does not cross react with human Wnt1, Wnt2, Wnt2b, Wnt3, Wnt3a, Wnt4, Wnt5a, Wnt7a, Wnt7b, Wnt8a, Wnt8b, Wnt9a, Wnt9b, Wnt10a, Wnt10b, and Wnt16 protein.
预测种属反应
Mouse, Bovine(>80% identity)
产品属性
形式
Liquid
存储溶液
PBS
存放说明
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
浓度
1 mg/ml (Please refer to the vial label for the specific concentration.)
免疫原种属
Human
免疫原
Carrier-protein conjugated synthetic peptide encompassing a sequence within the center region of human Wnt6. The exact sequence is proprietary.
纯化方式
Affinity purified by Protein A.
偶联
Unconjugated
注意事项
仅供实验室使用。不适用于人类或动物的任何临床，治疗或诊断用途。不适合动物或人类食用。
相关讯息
别名
Wnt family member 6
功能与背景
The WNT gene family consists of structurally related genes which encode secreted signaling proteins. These proteins have been implicated in oncogenesis and in several developmental processes, including regulation of cell fate and patterning during embryogenesis. This gene is a member of the WNT gene family. It is overexpressed in cervical cancer cell line and strongly coexpressed with another family member, WNT10A, in colorectal cancer cell line. The gene overexpression may play key roles in carcinogenesis. This gene and the WNT10A gene are clustered in the chromosome 2q35 region. The protein encoded by this gene is 97% identical to the mouse Wnt6 protein at the amino acid level. [provided by RefSeq, Jul 2008]
数据库
研究领域
產品图片
![Anti-Wnt6 antibody [HL1568] used in Western Blot (WB). GTX637043](/templates/default/js/lightbox/images/loading.gif "Anti-Wnt6 antibody [HL1568] used in Western Blot (WB). GTX637043")
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GTX637043 WB Image
Non-transfected (–) and transfected (+) 293T whole cell extracts were separated by 10% SDS-PAGE, and the membrane was blotted with Wnt6 antibody [HL1568] (GTX637043) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
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GTX637043 ICC/IF Image
Wnt6 antibody [HL1568] detects Wnt6 protein by immunofluorescent analysis.Sample: Mock and transfected 293T cells were fixed in 4% paraformaldehyde at RT for 15 min.Green: Wnt6 stained by Wnt6 antibody [HL1568] (GTX637043) diluted at 1:500.Blue: Fluoroshield with DAPI (GTX30920).
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GTX637043 IHC-P Image
Wnt6 antibody [HL1568] detects Wnt6 protein by immunohistochemical analysis.Sample: Paraffin-embedded rat adrenal gland.Wnt6 stained by Wnt6 antibody [HL1568] (GTX637043) diluted at 1:100.Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
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文献引用
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产品评论
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![Anti-Wnt6 antibody [HL1568] used in Western Blot (WB). GTX637043](/upload/website/prouct_img/small/GTX637043/GTX637043_44753_20230421_WB_multiple_B_23042500_503.webp "Anti-Wnt6 antibody [HL1568] used in Western Blot (WB). GTX637043")
![Anti-Wnt6 antibody [HL1568] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX637043](/upload/website/prouct_img/small/GTX637043/GTX637043_T-44704_20220916_ICC_IF_B_22102723_319.webp "Anti-Wnt6 antibody [HL1568] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX637043")
![Anti-Wnt6 antibody [HL1568] used in IHC (Paraffin sections) (IHC-P). GTX637043](/upload/website/prouct_img/small/GTX637043/GTX637043_44753_20221227_IHC-P_R_22122821_256.webp "Anti-Wnt6 antibody [HL1568] used in IHC (Paraffin sections) (IHC-P). GTX637043")
![Anti-Wnt6 antibody [HL1568] used in Western Blot (WB). GTX637043](/upload/website/prouct_img/normal/GTX637043/GTX637043_44753_20230421_WB_multiple_B_23042500_503.webp "Anti-Wnt6 antibody [HL1568] used in Western Blot (WB). GTX637043")
![Anti-Wnt6 antibody [HL1568] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX637043](/upload/website/prouct_img/normal/GTX637043/GTX637043_T-44704_20220916_ICC_IF_B_22102723_319.webp "Anti-Wnt6 antibody [HL1568] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX637043")
![Anti-Wnt6 antibody [HL1568] used in IHC (Paraffin sections) (IHC-P). GTX637043](/upload/website/prouct_img/normal/GTX637043/GTX637043_44753_20221227_IHC-P_R_22122821_256.webp "Anti-Wnt6 antibody [HL1568] used in IHC (Paraffin sections) (IHC-P). GTX637043")
