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XPC抗体 [3.26]

Anti-XPC antibody [3.26] used in Western Blot (WB). GTX70294
Anti-XPC antibody [3.26] used in Western Blot (WB). GTX70294
Anti-XPC antibody [3.26] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX70294

目录号 GTX70294

文献引用 20

宿主

 Mouse

克隆

 Monoclonal

克隆编号

 3.26

同种型

 IgG1

实验应用

 WB, ICC/IF, IHC-P

种属反应

 Human, Mouse
产品包装
100 μl (¥4300)
查看所有 XPC 的产品

产品应用

应用说明

*最佳稀释倍数与浓度应由研究人员确认
实验应用 稀释倍数
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P Assay dependent
WB: 免疫印迹
ICC/IF: 细胞染色
IHC-P: 石蜡切片
IHC-Fr: 冰冻切片

预期分子量

106 kDa. ( Note )

阳性对照

HeLa whole cell extract

产品属性

形式

Liquid

存储溶液

PBS

存放说明

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

浓度

1.15 mg/ml (Please refer to the vial label for the specific concentration.)

免疫原种属

Human

免疫原

Recombinant XPC purified from E. coli

纯化方式

Protein G purified

偶联

Unconjugated

RRID

AB_373299

注意事项

仅供实验室使用。不适用于人类或动物的任何临床，治疗或诊断用途。不适合动物或人类食用。

相关讯息

别名

XPC complex subunit, DNA damage recognition and repair factor , RAD4 , XP3 , XPCC , p125

细胞定位

Nucleus , Cytoplasm

功能与背景

The protein encoded by this gene is a key component of the XPC complex, which plays an important role in the early steps of global genome nucleotide excision repair (NER). The encoded protein is important for damage sensing and DNA binding, and shows a preference for single-stranded DNA. Mutations in this gene or some other NER components can result in Xeroderma pigmentosum, a rare autosomal recessive disorder characterized by increased sensitivity to sunlight with the development of carcinomas at an early age. Alternatively spliced transcript variants have been found for this gene. [provided by RefSeq, Aug 2017]

数据库

研究领域

產品图片

Anti-XPC antibody [3.26] used in Western Blot (WB). GTX70294

GTX70294 WB Image

Western blot image of human XPC from HeLa whole cell lysate detected using rabbit polyclonal (lanes 1 and 2, catalog numbers GTX70308 and GTX70309, respectively) or monoclonal (lane 3, catalog number GTX70294) antibodies.

Anti-XPC antibody [3.26] used in Western Blot (WB). GTX70294

GTX70294 WB Image

XPC antibody [3.26] detects XPC protein by western blot analysis. Whole cell extracts (30 μg) was separated by 5% SDS-PAGE, and the membrane was blotted with XPC antibody [3.26] (GTX70294) diluted at 1:500. The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody.

Anti-XPC antibody [3.26] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX70294

GTX70294 ICC/IF Image

XPC antibody [3.26] detects XPC protein at nucleus by immunofluorescent analysis.
Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: XPC protein stained by XPC antibody [3.26] (GTX70294) diluted at 1:100.
Red: Phalloidin, a cytoskeleton marker, diluted at 1:200.
Scale bar = 10 μm.

文献引用

Application Reference

de Semir D et al. Cancer Res 2021; (Epub) Nuclear receptor coactivator NCOA3 regulates ultraviolet radiation-induced DNA damage and melanoma susceptibility.
Wang YC et al. Cancers (Basel) 2020; 12 (8) Downregulation of the DNA Repair Gene DDB2 by Arecoline Is through p53's DNA-Binding Domain and Is Correlated with Poor Outcome of Head and Neck Cancer Patients with Betel Quid Consumption.
实验应用 : WB
种属反应 : Human
Pavey S et al. Mol Oncol 2020; 14 (1) : 22-41 Multiple interaction nodes define the postreplication repair response to UV-induced DNA damage that is defective in melanomas and correlated with UV signature mutation load.
实验应用 : WB
Sears CR et al. Am J Respir Cell Mol Biol 2017; (Epub) XPC Deficiency Alters Cigarette Smoke DNA Damage Cell Fate and Accelerates Emphysema Development.
实验应用 : WB
种属反应 : Mouse
Jia L et al. J Biol Chem 2016; (Epub) Dysregulation of CUL4A and CUL4B Ubiquitin Ligases in Lung Cancer.
实验应用 : IHC-P
种属反应 : Human
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Application Reference
de Semir D et al. Cancer Res 2021; (Epub) Nuclear receptor coactivator NCOA3 regulates ultraviolet radiation-induced DNA damage and melanoma susceptibility.
Wang YC et al. Cancers (Basel) 2020; 12 (8) Downregulation of the DNA Repair Gene DDB2 by Arecoline Is through p53's DNA-Binding Domain and Is Correlated with Poor Outcome of Head and Neck Cancer Patients with Betel Quid Consumption.
实验应用 : WB
种属反应 : Human
Pavey S et al. Mol Oncol 2020; 14 (1):22-41 Multiple interaction nodes define the postreplication repair response to UV-induced DNA damage that is defective in melanomas and correlated with UV signature mutation load.
实验应用 : WB
Sears CR et al. Am J Respir Cell Mol Biol 2017; (Epub) XPC Deficiency Alters Cigarette Smoke DNA Damage Cell Fate and Accelerates Emphysema Development.
实验应用 : WB
种属反应 : Mouse
Jia L et al. J Biol Chem 2016; (Epub) Dysregulation of CUL4A and CUL4B Ubiquitin Ligases in Lung Cancer.
实验应用 : IHC-P
种属反应 : Human
Dong TK et al. Photochem Photobiol 2016; 92 (5):760-6 Deficient Nucleotide Excision Repair in Squamous Cell Carcinoma Cells.
实验应用 : WB
种属反应 : Human
Kim H et al. Mol Cell 2014; 54 (1):107-18 Transcriptional Repressor ZBTB1 Promotes Chromatin Remodeling and Translesion DNA Synthesis.
实验应用 : ICC/IF
种属反应 : Human
Ramamoorthy M et al. Carcinogenesis 2013; 34 (10):2218-30 The RecQ helicase RECQL5 participates in psoralen-induced interstrand cross-link repair.
实验应用 : WB, ICC/IF
种属反应 : Human
Demetriou SK et al. Int J Cancer 2012; 131 (8):1818-27 Defective DNA repair and cell cycle arrest in cells expressing Merkel cell polyomavirus T antigen.
实验应用 : WB
种属反应 : Human
Acu ID et al. Cancer Res 2010; 70 (8):518859 Coordination of centrosome homeostasis and DNA repair is intact in MCF-7 and disrupted in MDA-MB 231 breast cancer cells.
实验应用 : ICC/IF
种属反应 : Human
Takedachi A et al. Mol Cell Biol 2010; 30 (11):2708-23 DDB2 complex-mediated ubiquitylation around DNA damage is oppositely regulated by XPC and Ku and contributes to the recruitment of XPA.
实验应用 : ICC/IF
种属反应 : Human
Rezvani HR et al. Nucleic Acids Res 2010; 38 (3):797-809 Hypoxia-inducible factor-1alpha regulates the expression of nucleotide excision repair proteins in keratinocytes.
实验应用 : WB
种属反应 : Human
de Feraudy S et al. Am J Pathol 2010; 177 (2):555-62 The DNA damage-binding protein XPC is a frequent target for inactivation in squamous cell carcinomas.
实验应用 : IHC-P
种属反应 : Human
Yan T et al. Clin Cancer Res 2009; 15 (22):6912-20 Differential cellular responses to prolonged LDR-IR in MLH1-proficient and MLH1-deficient colorectal cancer HCT116 cells.
实验应用 : WB
种属反应 : Human
Klein UR et al. J Cell Sci 2009; 122 (Pt 18):3312-21 SUMO-dependent regulation of centrin-2.
实验应用 : ICC/IF
种属反应 : Human
Ferguson-Yates BE et al. Carcinogenesis 2008; 29 (1):25689 Impaired repair of cyclobutane pyrimidine dimers in human keratinocytes deficient in p53 and p63.
实验应用 : WB
种属反应 : Human
Wang Y et al. Cancer Res 2008; 68 (10):723791 Repair of 2'-C-cyano-2'-deoxy-1-beta-D-arabino-pentofuranosylcytosine-induced DNA single-strand breaks by transcription-coupled nucleotide excision repair.
实验应用 : WB
种属反应 : Human
Laposa RR et al. Proc Natl Acad Sci U S A 2007; 104 (4):1389-94 Increased apoptosis, p53 up-regulation, and cerebellar neuronal degeneration in repair-deficient Cockayne syndrome mice.
实验应用 : IHC-P
种属反应 : Mouse
Ferguson BE et al. Cancer Res 2005; 65 (19):2492294 Proficient global nucleotide excision repair in human keratinocytes but not in fibroblasts deficient in p53.
实验应用 : WB
种属反应 : Human
Clone Reference
Adimoolam S et al. Proc Natl Acad Sci U S A 2002; 99 (20):12985-90 p53 and DNA damage-inducible expression of the xeroderma pigmentosum group C gene.
SDS
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产品包装 报价 (¥)
¥ 4300
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