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YTHDC2抗体 [HL1862]

Recombinant antibody Orthogonal
Anti-YTHDC2 antibody [HL1862] used in Western Blot (WB). GTX637587
Anti-YTHDC2 antibody [HL1862] used in IHC (Paraffin sections) (IHC-P). GTX637587
Anti-YTHDC2 antibody [HL1862] used in Western Blot (WB). GTX637587
Anti-YTHDC2 antibody [HL1862] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX637587

目录号 GTX637587

宿主

 Rabbit

克隆

 Monoclonal

克隆编号

 HL1862

同种型

 IgG

实验应用

 WB, ICC/IF, IHC-P

种属反应

 Human, Mouse
产品包装
100 μl (¥4000),
25 μl (¥1700)
查看所有 YTHDC2 的产品

产品应用

应用说明

*最佳稀释倍数与浓度应由研究人员确认
实验应用 稀释倍数
WB 1:500-1:3000
ICC/IF Assay dependent
IHC-P Assay dependent
WB: 免疫印迹
ICC/IF: 细胞染色
IHC-P: 石蜡切片
IHC-Fr: 冰冻切片

预期分子量

160 kDa. ( Note )

产品属性

形式

Liquid

存储溶液

PBS

存放说明

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

浓度

1 mg/ml (Please refer to the vial label for the specific concentration.)

免疫原种属

Human

免疫原

Recombinant protein encompassing a sequence within the C-terminus region of human YTHDC2. The exact sequence is proprietary.

纯化方式

Affinity purified by Protein A.

偶联

Unconjugated

注意事项

仅供实验室使用。不适用于人类或动物的任何临床，治疗或诊断用途。不适合动物或人类食用。

相关讯息

别名

YTH domain containing 2 , CAHL , hYTHDC2

细胞定位

Cytoplasm

功能与背景

This gene encodes a member of the DEAH (Asp-Glu-Ala-His) subfamily of proteins, part of the DEAD (Asp-Glu-Ala-Asp) box family of RNA helicases. The encoded protein binds to N6-methyladenosine, a common modified RNA nucleotide that is enriched in the stop codons and 3' UTRs of eukaryotic messenger RNAs. Binding of proteins to this modified nucleotide may regulate mRNA translation and stability. This gene may be associated with susceptibility to pancreatic cancer in human patients, and knockdown of this gene resulted in reduced proliferation in a human liver cancer cell line. [provided by RefSeq, Sep 2016]

数据库

研究领域

產品图片

Anti-YTHDC2 antibody [HL1862] used in Western Blot (WB). GTX637587

GTX637587 WB Image

Various whole cell extracts (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with YTHDC2 antibody [HL1862] (GTX637587) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-YTHDC2 antibody [HL1862] used in IHC (Paraffin sections) (IHC-P). GTX637587

GTX637587 IHC-P Image

YTHDC2 antibody [HL1862] detects YTHDC2 protein at cytoplasm by immunohistochemical analysis.
Sample: Paraffin-embedded mouse testis.
YTHDC2 stained by YTHDC2 antibody [HL1862] (GTX637587) diluted at 1:100.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-YTHDC2 antibody [HL1862] used in Western Blot (WB). GTX637587

GTX637587 WB Image

Orthogonal

Various whole cell extracts (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with YTHDC2 antibody [HL1862] (GTX637587) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody. Corresponding RNA expression data for the same cell lines are based on Human Protein Atlas program.

Anti-YTHDC2 antibody [HL1862] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX637587

GTX637587 ICC/IF Image

YTHDC2 antibody [HL1862] detects YTHDC2 protein at cytoplasm by immunofluorescent analysis.
Sample: HeLa cells were fixed in ice-cold MeOH for 5 min.
Green: YTHDC2 stained by YTHDC2 antibody [HL1862] (GTX637587) diluted at 1:200.
Blue: Fluoroshield with DAPI (GTX30920).

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产品包装 报价 (¥)
¥ 4000
¥ 1700
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