GeneTex中国官方网站
  • China

ZEB1抗体 [N2C1], Internal

KO/KD Comparison Orthogonal
Anti-ZEB1 antibody [N2C1], Internal used in Immunoprecipitation (IP). GTX105278
Anti-ZEB1 antibody [N2C1], Internal used in Western Blot (WB). GTX105278
Anti-ZEB1 antibody [N2C1], Internal used in Western Blot (WB). GTX105278
Anti-ZEB1 antibody [N2C1], Internal used in ChIP assay (ChIP assay). GTX105278
Anti-ZEB1 antibody [N2C1], Internal used in Western Blot (WB). GTX105278
Anti-ZEB1 antibody [N2C1], Internal used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX105278
Anti-ZEB1 antibody [N2C1], Internal used in Western Blot (WB). GTX105278
Anti-ZEB1 antibody [N2C1], Internal used in Western Blot (WB). GTX105278

目录号 GTX105278

文献引用 29
1

宿主

 Rabbit

克隆

 Polyclonal

同种型

 IgG

实验应用

 WB, ICC/IF, IP, ChIP assay

种属反应

 Human, Mouse
产品包装
100 μl (¥4000),
25 μl (¥1700)
查看所有 ZEB1 的产品

产品应用

应用说明

*最佳稀释倍数与浓度应由研究人员确认
实验应用 稀释倍数
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IP 1:100-1:500
ChIP assay Assay dependent
WB: 免疫印迹
ICC/IF: 细胞染色
IHC-P: 石蜡切片
IHC-Fr: 冰冻切片

预期分子量

124 kDa. ( Note )

阳性对照

293T , HeLa , HepG2 , Neuro2A , NIH-3T3 , C2C12

预测种属反应

Rat, Bovine, Chicken(>80% identity)

产品属性

形式

Liquid

存储溶液

PBS, 20% Glycerol

存放说明

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

浓度

0.1 mg/ml (Please refer to the vial label for the specific concentration.)

免疫原种属

Human

免疫原

Recombinant protein encompassing a sequence within the center region of human ZEB1. The exact sequence is proprietary.

纯化方式

Purified by antigen-affinity chromatography.

偶联

Unconjugated

RRID

AB_11162905

注意事项

仅供实验室使用。不适用于人类或动物的任何临床，治疗或诊断用途。不适合动物或人类食用。

相关讯息

别名

zinc finger E-box binding homeobox 1 , AREB6 , BZP , DELTAEF1 , FECD6 , NIL2A , PPCD3 , TCF8 , ZFHEP , ZFHX1A

细胞定位

Nucleus

功能与背景

ZEB1 encodes a zinc finger transcription factor that represses T-lymphocyte-specific IL2 gene (MIM 147680) expression by binding to a negative regulatory domain 100 nucleotides 5-prime of the IL2 transcription start site (Williams et al., 1991 [PubMed 1840704]).[supplied by OMIM]

数据库

研究领域

產品图片

Anti-ZEB1 antibody [N2C1], Internal used in Immunoprecipitation (IP). GTX105278

GTX105278 IP Image

ZEB1 antibody immunoprecipitates ZEB1 protein in IP experiments. IP Sample: 293T whole cell lysate/extract A. 40 μg 293T whole cell lysate/extract B. Control with 2 μg of preimmune rabbit IgG C. Immunoprecipitation of ZEB1 protein by 2 μg of ZEB1 antibody (GTX105278) 7.5% SDS-PAGE The immunoprecipitated ZEB1 protein was detected by ZEB1 antibody (GTX105278) diluted at 1:1000. EasyBlot anti-rabbit IgG (GTX221666-01) was used as a secondary reagent.

Anti-ZEB1 antibody [N2C1], Internal used in Western Blot (WB). GTX105278

GTX105278 WB Image

KO/KD

Non-transfected (–) and transfected (+) HeLa whole cell extracts (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with ZEB1 antibody [N2C1], Internal (GTX105278) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.

Anti-ZEB1 antibody [N2C1], Internal used in Western Blot (WB). GTX105278

GTX105278 WB Image

Comparison

Various whole cell extracts (30 μg) were separated by 5% SDS-PAGE, and the membranes were blotted with ZEB1 antibody [N2C1], Internal (GTX105278) diluted at 1:1000 and competitor's antibody diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

*The competitor is not affiliated with GeneTex and does not endorse this product.

Anti-ZEB1 antibody [N2C1], Internal used in ChIP assay (ChIP assay). GTX105278

GTX105278 ChIP assay Image

Cross-linked ChIP was performed with HeLa chromatin extract and 5 μg of either control rabbit IgG or anti-ZEB1 antibody. The precipitated DNA was detected by PCR with primer set targeting to LAMC2 promotor.
ChIP experiment and primer designs are based on Proc Natl Acad Sci U S A. 2011 Nov 29;108(48):19204-9.

Anti-ZEB1 antibody [N2C1], Internal used in Western Blot (WB). GTX105278

GTX105278 WB Image

Various whole cell extracts (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with ZEB1 antibody [N2C1], Internal (GTX105278) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
The observed M.W. is based on the publication: PMID: 19706487

Anti-ZEB1 antibody [N2C1], Internal used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX105278

GTX105278 ICC/IF Image

ZEB1 antibody [N2C1], Internal detects ZEB1 protein at nucleus by immunofluorescent analysis.
Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: ZEB1 protein stained by ZEB1 antibody [N2C1], Internal (GTX105278) diluted at 1:500.
Red: Phalloidin, a cytoskeleton marker, diluted at 1:100.
Scale bar = 10 μm.

Anti-ZEB1 antibody [N2C1], Internal used in Western Blot (WB). GTX105278

GTX105278 WB Image

Various whole cell extracts (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with ZEB1 antibody (GTX105278) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-ZEB1 antibody [N2C1], Internal used in Western Blot (WB). GTX105278

GTX105278 WB Image

Orthogonal

The data was published in the 2019 in Cancers (Basel). PMID: 31861872

文献引用

Application Reference

Runel G et al. Cancers (Basel) 2024; 16 (4) Cancer Cell Biomechanical Properties Accompany Tspan8-Dependent Cutaneous Melanoma Invasion.
G Runel et al. Cancers 2024; 16 (4) : 694 Cancer Cell Biomechanical Properties Accompany Tspan8-Dependent Cutaneous Melanoma Invasion
实验应用 : ChIP assay
TH Chang et al. Research Square 2023; The CCL2-CCR2 axis contributes to acquired osimertinib resistance in lung cancer by upregulating Zeb1 expression
实验应用 : WB
TH Hung et al. Biomed J 2023; : 100612 High expression of embryonic stem cell marker SSEA3 confers poor prognosis and promotes epithelial mesenchymal transition in hepatocellular carcinoma
实验应用 : WB
种属反应 : Human
PL Hsu et al. Sci Adv 2023; 9 (15) : eade3422 Targeting BRD3 eradicates nuclear TYRO3-induced colorectal cancer metastasis
实验应用 : WB
显示更多 +
我要回报文献

产品评论

ZEB1 antibody [N2C1], Internal 目录号 GTX105278

Rating
( Average 4 based on 1 users reviews)
实验应用
Western Blot(WB)
( Average 4 based on 1 users reviews)
Anti-ZEB1 antibody [N2C1], Internal used in Western Blot (WB). GTX105278
Date : Anonymous submitted on 07-Feb-2018
Score :
Application Tested : WB
Sample Species : Hu
Sample : H1975 cells
Amount used : 40 μg
Blocking : 5% milk, 25°C, 1Hr
Primary Antibody : 1:1000, 4°C, 16Hr
条带说明 : Lane 1: shLuc, Lane 2-4: shZEB1
我要评论产品
Application Reference
Runel G et al. Cancers (Basel) 2024; 16 (4) Cancer Cell Biomechanical Properties Accompany Tspan8-Dependent Cutaneous Melanoma Invasion.
G Runel et al. Cancers 2024; 16 (4):694 Cancer Cell Biomechanical Properties Accompany Tspan8-Dependent Cutaneous Melanoma Invasion
实验应用 : ChIP assay
TH Chang et al. Research Square 2023; The CCL2-CCR2 axis contributes to acquired osimertinib resistance in lung cancer by upregulating Zeb1 expression
实验应用 : WB
TH Hung et al. Biomed J 2023;:100612 High expression of embryonic stem cell marker SSEA3 confers poor prognosis and promotes epithelial mesenchymal transition in hepatocellular carcinoma
实验应用 : WB
种属反应 : Human
PL Hsu et al. Sci Adv 2023; 9 (15):eade3422 Targeting BRD3 eradicates nuclear TYRO3-induced colorectal cancer metastasis
实验应用 : WB
Chiou WC et al. Biomed Pharmacother 2023; 161:114481 Ovatodiolide inhibits SARS-CoV-2 replication and ameliorates pulmonary fibrosis through suppression of the TGF-β/TβRs signaling pathway.
实验应用 : WB
种属反应 : Human
Wuputra K et al. Int J Mol Sci 2022; 23 (14) FOXM1-CD44 Signaling Is Critical for the Acquisition of Regorafenib Resistance in Human Liver Cancer Cells.
Plaschka M et al. J Immunother Cancer 2022; 10 (3) ZEB1 transcription factor promotes immune escape in melanoma.
实验应用 : IP
Jie-Ning Li et al. Front Cell Dev Biol 2021; 9:671244 E-cadherin Interacts With Posttranslationally-Modified AGO2 to Enhance miRISC Activity.
实验应用 : WB
种属反应 : Human
Tsai SC et al. Onco Targets Ther 2021; 14:4523-4536 Tetrandrine Inhibits Epithelial-Mesenchymal Transition in IL-6-Induced HCT116 Human Colorectal Cancer Cells.
Wang YY et al. Cancers (Basel) 2020; 12 (12) Visfatin Enhances Breast Cancer Progression through CXCL1 Induction in Tumor-Associated Macrophages.
实验应用 : WB
种属反应 : Human
Li CL et al. Anticancer Res 2020; 40 (10):5529-5538 Curcumol Suppresses Triple-negative Breast Cancer Metastasis by Attenuating Anoikis Resistance via Inhibition of Skp2-mediated Transcriptional Addiction.
实验应用 : WB
种属反应 : Human
Lee JY et al. PLoS One 2020; 15 (7):e0236876 PHRF1 promotes migration and invasion by modulating ZEB1 expression.
实验应用 : WB, IP
种属反应 : Human
Fang CH et al. J Biomed Sci 2020; 27 (1):42 A novel c-Kit/phospho-prohibitin axis enhances ovarian cancer stemness and chemoresistance via Notch3-PBX1 and 帣-catenin-ABCG2 signaling.
实验应用 : WB
种属反应 : Human
Pei YF et al. Am J Pathol 2020; 190 (6):1343-1354 The Crosstalk between lncRNA-SNHG7/miRNA-181/cbx7 Modulates Malignant Character in Lung Adenocarcinoma.
实验应用 : WB
种属反应 : Human
Huang JY et al. Cancers (Basel) 2019; 12 (1) Visfatin Mediates Malignant Behaviors through Adipose-Derived Stem Cells Intermediary in Breast Cancer.
实验应用 : WB
种属反应 : Human
Chen Q et al. Mol Cancer Res 2019; (Epub)
实验应用 : IP
Liu G et al. Cell Cycle 2019; 18 (9):1033-1044 Transcription elements AREB6 and miR-34a affect apoptosis of PAMs by regulating the expression of SS2-related gene PPP1R11.
实验应用 : ChIP assay
Yoriki K et al. Sci Rep 2019; 9 (1):6697 Estrogen-related receptor alpha induces epithelial-mesenchymal transition through cancer-stromal interactions in endometrial cancer.
实验应用 : WB
种属反应 : Human
Pei YF et al. Am J Pathol 2019; (Epub) Methyl-CpG Binding Domain Protein 2 Inhibits the Malignant Characteristic of Lung Adenocarcinoma through the Epigenetic Modulation of 10 to 11 Translocation 1 and miR-200s.
实验应用 : WB
种属反应 : Human
Chang CY et al. Cell Death Discov 2018; 4:100 PEGylated liposome-encapsulated rhenium-188 radiopharmaceutical inhibits proliferation and epithelial-mesenchymal transition of human head and neck cancer cells in vivo with repeated therapy.
实验应用 : WB
种属反应 : Mouse
Ryu D et al. Biomol Ther (Seoul) 2018; 26 (5):487-493 Overexpression of CD44 Standard Isoform Upregulates HIF-1α Signaling in Hypoxic Breast Cancer Cells.
实验应用 : WB
种属反应 : Human
Wang PS et al. Oncogene 2018; (Epub) A novel long non-coding RNA linc-ZNF469-3 promotes lung metastasis through miR-574-5p-ZEB1 axis in triple negative breast cancer.
实验应用 : WB
种属反应 : Human
Pei YF et al. Biochim Biophys Acta 2017; (Epub) TOP2A induces malignant character of pancreatic cancer through activating β-catenin signaling pathway.
实验应用 : WB
种属反应 : Human
Zou Q et al. J Cell Biochem 2017; (Epub) A TP73-AS1/miR-200a/ZEB1 regulating loop promotes breast cancer cell invasion and migration.
实验应用 : WB, ChIP assay
种属反应 : Human
Morel AP et al. Nat Med 2017; (Epub) A stemness-related ZEB1-MSRB3 axis governs cellular pliancy and breast cancer genome stability.
实验应用 : IP
种属反应 : Human
Wang LT et al. Oncogene 2017; (Epub) Transcription factor SPZ1 promotes TWIST-mediated epithelial-mesenchymal transition and oncogenesis in human liver cancer.
实验应用 : WB
种属反应 : Human
Yin K et al. Oncotarget 2016; 7 (17):24537-48 MiR-206 suppresses epithelial mesenchymal transition by targeting TGF-β signaling in estrogen receptor positive breast cancer cells.
实验应用 : WB
种属反应 : Human
Chiu WC et al. PLoS One 2016; 11 (11):e0166453 The Synthetic β-Nitrostyrene Derivative CYT-Rx20 Inhibits Esophageal Tumor Growth and Metastasis via PI3K/AKT and STAT3 Pathways.
实验应用 : WB
种属反应 : Human
SDS
PBS.pdf
Glycerol.pdf
Proclin.pdf
产品包装 报价 (¥)
¥ 4000
¥ 1700
联系经销商
欣博盛生物科技有限公司
欣博盛生物科技有限公司
(全国热线) 4006-800-892 / (深圳) 0755-26755892 / (北京) 010-88594029 / (上海) 021-34613729 / (广州) 18024516375 / (香港) +852-69410778
http://www.neobioscience.com
genetex@neobioscience.com