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ZEB2抗体

Orthogonal Overexpression
Anti-ZEB2 antibody used in Western Blot (WB). GTX129243
Anti-ZEB2 antibody used in IHC (Paraffin sections) (IHC-P). GTX129243
Anti-ZEB2 antibody used in Western Blot (WB). GTX129243
Anti-ZEB2 antibody used in Western Blot (WB). GTX129243
Anti-ZEB2 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX129243
Anti-ZEB2 antibody used in Western Blot (WB). GTX129243
Anti-ZEB2 antibody used in Western Blot (WB). GTX129243

目录号 GTX129243

文献引用 7

宿主

 Rabbit

克隆

 Polyclonal

同种型

 IgG

实验应用

 WB, ICC/IF, IHC-P

种属反应

 Human, Mouse
产品包装
100 μl (¥4000),
25 μl (¥1700)
查看所有 ZEB2 的产品

产品应用

应用说明

*最佳稀释倍数与浓度应由研究人员确认
实验应用 稀释倍数
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P Assay dependent
WB: 免疫印迹
ICC/IF: 细胞染色
IHC-P: 石蜡切片
IHC-Fr: 冰冻切片

预期分子量

136 kDa. ( Note )

阳性对照

DDDDK-tagged ZEB2-transfected 293T

预测种属反应

Rat, Bovine, Chicken(>80% identity)

产品属性

形式

Liquid

存储溶液

PBS, 1% BSA, 20% Glycerol

存放说明

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

浓度

0.42 mg/ml (Please refer to the vial label for the specific concentration.)

免疫原种属

Human

免疫原

Recombinant protein encompassing a sequence within the center region of human ZEB2. The exact sequence is proprietary.

纯化方式

Purified by antigen-affinity chromatography.

偶联

Unconjugated

RRID

AB_2885939

注意事项

仅供实验室使用。不适用于人类或动物的任何临床，治疗或诊断用途。不适合动物或人类食用。

相关讯息

别名

zinc finger E-box binding homeobox 2 , HSPC082 , SIP-1 , SIP1 , SMADIP1 , ZFHX1B

细胞定位

Nucleus

功能与背景

The SMADIP1 gene (also known as SIP1) is a member of the delta-EF1 (ZEB1; MIM 189909)/Zfh1 family of 2-handed zinc finger/homeodomain proteins. SMADIP1 interacts with receptor-mediated, activated full-length SMADs (see MIM 605568) (Verschueren et al., 1999 [PubMed 10400677]).[supplied by OMIM]

数据库

研究领域

產品图片

Anti-ZEB2 antibody used in Western Blot (WB). GTX129243

GTX129243 WB Image

Various whole cell extracts (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with ZEB2 antibody (GTX129243) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.

Anti-ZEB2 antibody used in IHC (Paraffin sections) (IHC-P). GTX129243

GTX129243 IHC-P Image

ZEB2 antibody detects ZEB2 protein at nucleus by immunohistochemical analysis.
Sample: Paraffin-embedded mouse spleen.
ZEB2 stained by ZEB2 antibody (GTX129243) diluted at 1:1000.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-ZEB2 antibody used in Western Blot (WB). GTX129243

GTX129243 WB Image

Whole cell extract (30 μg) was separated by 5% SDS-PAGE, and the membrane was blotted with ZEB2 antibody (GTX129243) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-ZEB2 antibody used in Western Blot (WB). GTX129243

GTX129243 WB Image

Mouse tissue extract (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with ZEB2 antibody (GTX129243) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.

Anti-ZEB2 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX129243

GTX129243 ICC/IF Image

ZEB2 antibody detects ZEB2 protein at nucleus by immunofluorescent analysis.
Sample: U87-MG cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: ZEB2 stained by ZEB2 antibody (GTX129243) diluted at 1:500.
Red: alpha Tubulin, a cytoskeleton marker, stained by alpha Tubulin antibody [GT114] (GTX628802) diluted at 1:1000.

Anti-ZEB2 antibody used in Western Blot (WB). GTX129243

GTX129243 WB Image

Overexpression

Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with ZEB2 antibody (GTX129243) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-ZEB2 antibody used in Western Blot (WB). GTX129243

GTX129243 WB Image

Orthogonal

The data was published in the journal Cancer Med in 2020.PMID: 32277605

文献引用

Application Reference

Ji Y et al. Cancer Med 2020; 9 (11) : 3954-3963 Long noncoding RNA MEG3 decreases the growth of head and neck squamous cell carcinoma by regulating the expression of miR-421 and E-cadherin.
Lee JY et al. PLoS One 2020; 15 (7) : e0236876 PHRF1 promotes migration and invasion by modulating ZEB1 expression.
实验应用 : WB
种属反应 : Human
Pei YF et al. Am J Pathol 2020; 190 (6) : 1343-1354 The Crosstalk between lncRNA-SNHG7/miRNA-181/cbx7 Modulates Malignant Character in Lung Adenocarcinoma.
实验应用 : WB
种属反应 : Human
Pei YF et al. Am J Pathol 2019; (Epub) Methyl-CpG Binding Domain Protein 2 Inhibits the Malignant Characteristic of Lung Adenocarcinoma through the Epigenetic Modulation of 10 to 11 Translocation 1 and miR-200s.
实验应用 : WB
种属反应 : Human
Li JM et al. Ther Adv Med Oncol 2018; 10 : 1.76E+15 Upregulation of LGALS1 is associated with oral cancer metastasis.
实验应用 : WB
种属反应 : Human
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SDS
PBS.pdf
Glycerol.pdf
Proclin.pdf
产品包装 报价 (¥)
¥ 4000
¥ 1700
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